Human chymase stimulates Ca2+ signaling in human polymorphonuclear cells

• Published in: Immunology letters Vol. 89; no. 2-3; pp. 161 - 165
• Main Authors: Saito, Kayo, Muto, Tsuyoshi, Tomimori, Yoshiaki, Maruoka, Hiroshi, Tanaka, Taisaku, Fukuda, Yoshiaki
• Format: Journal Article
• Language: English
• Published: Netherlands 31.10.2003
• Subjects: Calcium - metabolism; Calcium Signaling - drug effects; Calcium Signaling - physiology; Chymases; Enzyme Inhibitors - pharmacology; Humans; Neutrophils - drug effects; Neutrophils - metabolism; Pertussis Toxin - pharmacology; Serine Endopeptidases - drug effects; Serine Endopeptidases - metabolism

Human chymase is known to function as a chemoattractant for human leukocytes. To investigate the mechanism of the chymase-induced cell migration, change in intracellular calcium concentration ([Ca(2+)]i) was examined in human polymorphonuclear (PMN) cells using Fluo-3 as a fluorescent Ca(2+) indicat...