The effects of Initial Preparation on the Composition of the Subgingival Microbial Flora in Adult Periodontitis
The purpose of this study was to examine the relative proportions of subgingival microbial flora in periodontally diseased patients before and after initial preparation. Plaque control, subgingival scaling and root planing were carried out in nine subjects with adult periodontitis ranged from 4 to 8...
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| Published in | Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Vol. 26; no. 2; pp. 329 - 338 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
JAPANESE SOCIETY OF PERIODONTOLOGY
1984
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0385-0110 1880-408X |
| DOI | 10.2329/perio.26.329 |
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| Abstract | The purpose of this study was to examine the relative proportions of subgingival microbial flora in periodontally diseased patients before and after initial preparation. Plaque control, subgingival scaling and root planing were carried out in nine subjects with adult periodontitis ranged from 4 to 8mm pockets. The bacterial samples were obtained and subjected to Gram stain, dark field microscopy and an anaerobic incubation prior to treatment, after plaque control, and after scaling and root planing. The relative number of Gram positive cocci increased while Gram negative rods and filaments decreased after initial treatment. The mean percentage of Fusobacterium was 6.8%, 4.8% and 1.4%. Bacteroides was 11.0%, 3.1% and 0.1%, Spirochetes was 11.0%, 9.5% and 0.5%, respectively, black-pigmented Bacteroides and Fusobacterium necrophorum could be characterized and identified by the analyses of the gas chromatography and “Anaerobic Laboratory Manual”. A positive correlation was observed between Bacteroides and probing depth. Spirochetes closely correlated with probing depth, gingival index and plaque score as clinical parameters. |
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| AbstractList | The purpose of this study was to examine the relative proportions of subgingival microbial flora in periodontally diseased patients before and after initial preparation. Plaque control, subgingival scaling and root planing were carried out in nine subjects with adult periodontitis ranged from 4 to 8mm pockets. The bacterial samples were obtained and subjected to Gram stain, dark field microscopy and an anaerobic incubation prior to treatment, after plaque control, and after scaling and root planing. The relative number of Gram positive cocci increased while Gram negative rods and filaments decreased after initial treatment. The mean percentage of Fusobacterium was 6.8%, 4.8% and 1.4%. Bacteroides was 11.0%, 3.1% and 0.1%, Spirochetes was 11.0%, 9.5% and 0.5%, respectively, black-pigmented Bacteroides and Fusobacterium necrophorum could be characterized and identified by the analyses of the gas chromatography and “Anaerobic Laboratory Manual”. A positive correlation was observed between Bacteroides and probing depth. Spirochetes closely correlated with probing depth, gingival index and plaque score as clinical parameters. |
| Author | BOKU, Hideki KAMIYA, Hiroyuki HARA, Kohji EGUCHI, Taiko YOSHIE, Hiromasa OKUDA, Kazuhiro HASEGAWA, Eiko |
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| References | 1) Tanner, A. C. R., Haffer, C., Bratthall, G. T., Visconti, R. A. and Socransky, S. S.: A study of the bacteria associated with advancing periodontitis in man. J. Clin. Periodontol., 6: 278-307, 1979. 13) Löe, H.: The gingival index, the plaque index and the retention index systems. J. Periodontol., 38: 610-616, 1967. 22) Zambon, J. J., Reynolds, H. S. and Slots, J.: Black-pigmented Bacteroides spp. in the human oral cavity. Infect. Immun., 32: 198-203, 1981. 20) Slots, J., Mashimo, P., Levine, M. J. and Genco, R. J.: Periodontal therapy in humans. I. Microbiological and clinical effects of a single course of periodontal scaling and root planing, and of adjunctive tetracycline therapy. J. Periodontol., 50: 495-509, 1979. 25) Listgarten, M. A. and Schifter, C.: Differential dark field microscopy of subgingival bacteria as an aid in selecting recall intervals: results after 18 months. J. Clin. Periodontol., 9: 305-316, 1982. 15) 医科学研究所学友会編: 細菌学実習提要. 5版, 丸善株式会社, 東京, 1977, 123. 4) Rosenberg, E. S., Evian, C. I. and Listgarten, M. A.: The composition of the subgingival microbiota after periodontal therapy. J. Periodontol., 52: 435-441, 1981. 21) Slots, J.: The predominant cultivable microflora of advanced periodontitis. Scand. J. Dent. Res., 85: 114-121, 1977. 6) Siegrist, B. and Kornman, K. S.: The effect of supragingival plaque control on the composition of the subgingival microbial flora in ligature-induced periodontitis in the monkey. J. Dent. Res., 61: 936-941, 1982. 16) Listgarten, M. A. and Hellden, L.: Relative distribution of bacteria at clinically healthy and periodontally disease sites in humans. J. Clin. Periodontol., 5: 115-132, 1978. 24) Kornman, K. S., Siegrist, B., Soskolne, W. A. and Nuki, K.: The predominant cultivable subgingival flora of beagle dogs following ligature placement and metronidazole therapy. J. Periodont. Res., 16: 251-258, 1981. 7) Singletary, M. M., Crawford, J. J. and Simpson, D. M.: Dark-field microscopic monitoring of subgingival bacteria during periodontal therapy. J. Periodontol., 53: 671-681, 1982. 11) Listgarten, M. A., Lindhe, J. and Hellden, L.: Effect of tetracycline and/or scaling on human periodontal disease. J. Clin. Periodontol., 5: 246-271, 1978. 10) 井上純一, 本城範典, 山岡昭, 福島久典, 梅本俊夫, 佐川寛典: Advancing periodontitis 患者の歯周ポケット内細菌叢の検索-歯周外科処置後の細菌叢-. 日歯周誌, 25: 605, 1983. 23) Kornman, K. S., Holt, S. C. and Robertson, P. B.: The microbiology of ligature-induced periodontitis in the cynomolgus monkey. J. Periodont. Res., 16: 363-371, 1981. 17) Holdman, L. V. and Moore, W. E. C: Anaerobic laboratory manual. Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 1973. 14) O'Leary, T. J., Drake, R. B. and Naylor, J. E.: The plaque control record. J. Periodontol., 43: 38-40, 1972. 8) 本城範典, 山岡昭, 福島久典, 梅本俊夫, 佐川寛典: Advancing Periodontitis 患者の歯周ポケット内細菌叢の検索-歯周初期治療後の細菌叢-. 日歯周誌, 25: 432, 1983. 18) 鈴木祥一郎, 上野一恵監修: 臨床嫌気性菌マニュアル. 日水製薬株式会社, 東京, 1979, 37-38. 19) Mousques, T., Listgarten, M. A. and Phillips, R. W.: Effect of scaling and root planing on the composition of the human subgingival microbial flora. J. Periodont. Res., 15: 144-151, 1980. 12) Tsuchida, K. and Hara, K.: Clinical significance of gingival fluid measurement by “Periotron”. J. Periodontol., 52: 697-700, 1981. 9) 佃宣和, 紺野純一, 川浪雅光, 内山純一, 塚本晃也, 木葉篤, 白石ゆかり, 石川純: ブラッシングに伴う歯周ポケット内細菌叢の変化, 日歯周誌, 25: 545-553, 1983. 5) Syed, S. A., Morrison, E. C. and Lang, N. P.: Effects of repeated scaling and root planing and/or controlled oral hygiene on the periodontal attachment level and pocket depths in beagle dogs. II. Bacteriological findings. J. Periodont. Res., 17: 219-225, 1982. 2) Lindhe, J., Liljenberg, B. and Listgarten, M. A.: Some microbiological and histopathological features of periodontal disease in man. J. Periodontol., 51: 264-269, 1980. 3) Evian, C. I. Rosenberg, E. S. and Listgarten, M. A.: Bacterial variability within diseased periodontal sites. J. Periodontol., 53: 595-598, 1982. |
| References_xml | – reference: 17) Holdman, L. V. and Moore, W. E. C: Anaerobic laboratory manual. Virginia Polytechnic Institute and State University, Blacksburg, Virginia, 1973. – reference: 12) Tsuchida, K. and Hara, K.: Clinical significance of gingival fluid measurement by “Periotron”. J. Periodontol., 52: 697-700, 1981. – reference: 21) Slots, J.: The predominant cultivable microflora of advanced periodontitis. Scand. J. Dent. Res., 85: 114-121, 1977. – reference: 15) 医科学研究所学友会編: 細菌学実習提要. 5版, 丸善株式会社, 東京, 1977, 123. – reference: 22) Zambon, J. J., Reynolds, H. S. and Slots, J.: Black-pigmented Bacteroides spp. in the human oral cavity. Infect. Immun., 32: 198-203, 1981. – reference: 16) Listgarten, M. A. and Hellden, L.: Relative distribution of bacteria at clinically healthy and periodontally disease sites in humans. J. Clin. Periodontol., 5: 115-132, 1978. – reference: 20) Slots, J., Mashimo, P., Levine, M. J. and Genco, R. J.: Periodontal therapy in humans. I. Microbiological and clinical effects of a single course of periodontal scaling and root planing, and of adjunctive tetracycline therapy. J. Periodontol., 50: 495-509, 1979. – reference: 5) Syed, S. A., Morrison, E. C. and Lang, N. P.: Effects of repeated scaling and root planing and/or controlled oral hygiene on the periodontal attachment level and pocket depths in beagle dogs. II. Bacteriological findings. J. Periodont. Res., 17: 219-225, 1982. – reference: 25) Listgarten, M. A. and Schifter, C.: Differential dark field microscopy of subgingival bacteria as an aid in selecting recall intervals: results after 18 months. J. Clin. Periodontol., 9: 305-316, 1982. – reference: 18) 鈴木祥一郎, 上野一恵監修: 臨床嫌気性菌マニュアル. 日水製薬株式会社, 東京, 1979, 37-38. – reference: 23) Kornman, K. S., Holt, S. C. and Robertson, P. B.: The microbiology of ligature-induced periodontitis in the cynomolgus monkey. J. Periodont. Res., 16: 363-371, 1981. – reference: 19) Mousques, T., Listgarten, M. A. and Phillips, R. W.: Effect of scaling and root planing on the composition of the human subgingival microbial flora. J. Periodont. Res., 15: 144-151, 1980. – reference: 8) 本城範典, 山岡昭, 福島久典, 梅本俊夫, 佐川寛典: Advancing Periodontitis 患者の歯周ポケット内細菌叢の検索-歯周初期治療後の細菌叢-. 日歯周誌, 25: 432, 1983. – reference: 2) Lindhe, J., Liljenberg, B. and Listgarten, M. A.: Some microbiological and histopathological features of periodontal disease in man. J. Periodontol., 51: 264-269, 1980. – reference: 6) Siegrist, B. and Kornman, K. S.: The effect of supragingival plaque control on the composition of the subgingival microbial flora in ligature-induced periodontitis in the monkey. J. Dent. Res., 61: 936-941, 1982. – reference: 1) Tanner, A. C. R., Haffer, C., Bratthall, G. T., Visconti, R. A. and Socransky, S. S.: A study of the bacteria associated with advancing periodontitis in man. J. Clin. Periodontol., 6: 278-307, 1979. – reference: 13) Löe, H.: The gingival index, the plaque index and the retention index systems. J. Periodontol., 38: 610-616, 1967. – reference: 14) O'Leary, T. J., Drake, R. B. and Naylor, J. E.: The plaque control record. J. Periodontol., 43: 38-40, 1972. – reference: 24) Kornman, K. S., Siegrist, B., Soskolne, W. A. and Nuki, K.: The predominant cultivable subgingival flora of beagle dogs following ligature placement and metronidazole therapy. J. Periodont. Res., 16: 251-258, 1981. – reference: 10) 井上純一, 本城範典, 山岡昭, 福島久典, 梅本俊夫, 佐川寛典: Advancing periodontitis 患者の歯周ポケット内細菌叢の検索-歯周外科処置後の細菌叢-. 日歯周誌, 25: 605, 1983. – reference: 3) Evian, C. I. Rosenberg, E. S. and Listgarten, M. A.: Bacterial variability within diseased periodontal sites. J. Periodontol., 53: 595-598, 1982. – reference: 9) 佃宣和, 紺野純一, 川浪雅光, 内山純一, 塚本晃也, 木葉篤, 白石ゆかり, 石川純: ブラッシングに伴う歯周ポケット内細菌叢の変化, 日歯周誌, 25: 545-553, 1983. – reference: 7) Singletary, M. M., Crawford, J. J. and Simpson, D. M.: Dark-field microscopic monitoring of subgingival bacteria during periodontal therapy. J. Periodontol., 53: 671-681, 1982. – reference: 11) Listgarten, M. A., Lindhe, J. and Hellden, L.: Effect of tetracycline and/or scaling on human periodontal disease. J. Clin. Periodontol., 5: 246-271, 1978. – reference: 4) Rosenberg, E. S., Evian, C. I. and Listgarten, M. A.: The composition of the subgingival microbiota after periodontal therapy. J. Periodontol., 52: 435-441, 1981. |
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| SubjectTerms | Initial preparation Microbial flora Periodontal pocket |
| Title | The effects of Initial Preparation on the Composition of the Subgingival Microbial Flora in Adult Periodontitis |
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