Digestibility of Water-soluble Fibroin Prepared from Silk Yarn
Digestibility of water-soluble fibroin has long remained unknown. The present study thus aimed to clarify the digestibility of water-soluble fibroin extracted from silk yarn, and to determine the proximate composition and amino acid composition. In vitro digestibility of water-soluble fibroin was 58...
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| Published in | Nihon Eiyō, Shokuryō Gakkai shi Vol. 57; no. 5; pp. 215 - 220 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
Japan Society of Nutrition and Food Science
2004
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0287-3516 1883-2849 |
| DOI | 10.4327/jsnfs.57.215 |
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| Abstract | Digestibility of water-soluble fibroin has long remained unknown. The present study thus aimed to clarify the digestibility of water-soluble fibroin extracted from silk yarn, and to determine the proximate composition and amino acid composition. In vitro digestibility of water-soluble fibroin was 58% after treatment with pepsin-trypsin-chymotrypsin. In contrast, in vivo true digestibility (TD) of water-soluble fibroin was 65.7% in rats. Moreover, water-soluble fibroin exhibited lower food efficiency, TD, protein efficiency ratio, biological value, and net protein utilization as a protein source than the casein group. The present study has thus clarified the digestibility of water-soluble fibroin. |
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| AbstractList | Digestibility of water-soluble fibroin has long remained unknown. The present study thus aimed to clarify the digestibility of water-soluble fibroin extracted from silk yarn, and to determine the proximate composition and amino acid composition. In vitro digestibility of water-soluble fibroin was 58% after treatment with pepsin-trypsin-chymotrypsin. In contrast, in vivo true digestibility (TD) of water-soluble fibroin was 65.7% in rats. Moreover, water-soluble fibroin exhibited lower food efficiency, TD, protein efficiency ratio, biological value, and net protein utilization as a protein source than the casein group. The present study has thus clarified the digestibility of water-soluble fibroin. |
| Author | Yamamoto, Yuji Minagawa, Hirohisa Maekawa, Akio Tadokoro, Tadahiro |
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| References | 15) Okuyama K, Somashekar R, Noguchi K, Ichimura S (2001) Refined molecular and crystal structure of silk I based on Ala-Gly and (Ala-Gly) 2-Ser-Gly peptide sequence. Biopolymers 59 310-9. 18) Sugiyama K, Ohishi A, Ohnuma Y, Muramatsu K (1989) Composition between the plasma cholesterol lowering effects of glycine and taurine in rats fed on high cholesterol diet. Agric Biol Chem 53: 1647-52. 21) 谷口宏吉, 冨田次男, 山止康弘 (1997) 絹蛋白質由来アンギオテンシンI変換酵素阻害ペプチドに関する研究. 明治大学農学部研究報告112, 1-19. 9) 後藤逸男, 村本穣司, 蜷木翠 (1992) テフロン加圧分解容器-ICP発光分光分析法による植物の無機成分分析. 日本土壌肥料学雑誌 63, 345-8. 19) 陳開利, 井浦克弘, 高野亮, 平林潔 (1993) ラット血中コレステロール濃度に対するフィブロイン投与の効果. 日本蚕糸学雑誌 62, 56-60. 20) 平林潔, 陳開利, 勢籏毅 (1991) 絹フィブロインの食品素材としての活用. New Food Ind. 33: 1-4. 11) 弘田玲, 白鳥恭子, 中川滋木 (1998) 投与ペプチドの濃度変化による小腸内オルニチントランスカルバミラーゼ (OTC) への影響. 消化と吸収 21, 16-9. 22) Kato N, Iwami K (2002) Resistant protein; Its existence and function beneficial to health. J Nutr Sci Vitaminol 48: 1-5. 6) 安田勝利 (1996) 絹蛋白質の新利用技術に関する試験 (第1報). 岐阜蚕糸研要報 6, 46-56. 10) Terahara M, Nishide S, Kaneko T (2000) Effect of Streptococcus thermophilus on the Trp-P-1 level in the blood. Biosci Biotechnol Biochem 64: 1531-3. 7) AOAC (1990) Official Methods of Analysis of the AOAC 15th Edition, p. 777-82. AOAC, Arlington. 8) 後藤逸男, 蜷木翠 (1991) メタホウ酸リチウム溶融-ICP発光分光分析法による植物の無機成分分析. 日本土壌肥料学雑誌 62, 628-32. 1) シルクサイエンス研究会編 (1994) シルクの科学, p. 7-9, p. 87-96, p. 113-23. 朝倉書店, 東京 5) 平林潔 (1989) 絹の可溶化とその応用. Bio Ind. 6: 749-54. 2) 陳開利, 高野亮, 平林潔 (1991) 塩酸加水分解による水溶性絹粉末の作製とその物性. 日本蚕糸学雑誌 60, 358-62. 16) Hsu HW, Vavak DL, Satterlee LD, Miller GA (1977) A multienzyme technique for estimating protein digestibility. J Food Sci 42: 1269-77. 14) McLaughlan JM, Anderson GH, Hackler LR, Hill DC, Jansen GR, Keith MO, Sarwar G, Sosulski FW (1980) Assessment of rat growth methods for estimating protein quality: Interlaboratory study. J Assoc Off Anal Chem 63: 462-7. 13) Reeves PG, Nielsen FH, Fahey GC Jr (1993) AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 123: 1939-51. 3) 陳開利, 井浦克弘, 相沢竜司, 平林潔 (1991) 加水分解した絹蛋白質の消化. 日本蚕糸学雑誌 60, 402-3. 4) 平尾和子, 塚越幸子, 五十嵐喜治 (1999) 絹フィブロイン起泡粉末の給与がラットの血清コレステロール濃度に及ぼす影響. 日本栄養・食糧学会誌 52, 219-23. 17) Sugiyama K, Kushima Y, Muramatsu K (1985) Effect of sulfur-containing amino acid and glycine on plasma cholesterol level in rats fed on a high cholesterol diet. Agric Biol Chem 49: 3455-61. 12) Odani S, Awatuhara H, Kato Y (1997) Antigenic change of native and heat-denatured ovalbumin digested with pepsin, trypsin, chymotrypsin. J Home Econ Jpn 48: 717-22. |
| References_xml | – reference: 6) 安田勝利 (1996) 絹蛋白質の新利用技術に関する試験 (第1報). 岐阜蚕糸研要報 6, 46-56. – reference: 19) 陳開利, 井浦克弘, 高野亮, 平林潔 (1993) ラット血中コレステロール濃度に対するフィブロイン投与の効果. 日本蚕糸学雑誌 62, 56-60. – reference: 2) 陳開利, 高野亮, 平林潔 (1991) 塩酸加水分解による水溶性絹粉末の作製とその物性. 日本蚕糸学雑誌 60, 358-62. – reference: 10) Terahara M, Nishide S, Kaneko T (2000) Effect of Streptococcus thermophilus on the Trp-P-1 level in the blood. Biosci Biotechnol Biochem 64: 1531-3. – reference: 13) Reeves PG, Nielsen FH, Fahey GC Jr (1993) AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 123: 1939-51. – reference: 12) Odani S, Awatuhara H, Kato Y (1997) Antigenic change of native and heat-denatured ovalbumin digested with pepsin, trypsin, chymotrypsin. J Home Econ Jpn 48: 717-22. – reference: 15) Okuyama K, Somashekar R, Noguchi K, Ichimura S (2001) Refined molecular and crystal structure of silk I based on Ala-Gly and (Ala-Gly) 2-Ser-Gly peptide sequence. Biopolymers 59 310-9. – reference: 18) Sugiyama K, Ohishi A, Ohnuma Y, Muramatsu K (1989) Composition between the plasma cholesterol lowering effects of glycine and taurine in rats fed on high cholesterol diet. Agric Biol Chem 53: 1647-52. – reference: 22) Kato N, Iwami K (2002) Resistant protein; Its existence and function beneficial to health. J Nutr Sci Vitaminol 48: 1-5. – reference: 3) 陳開利, 井浦克弘, 相沢竜司, 平林潔 (1991) 加水分解した絹蛋白質の消化. 日本蚕糸学雑誌 60, 402-3. – reference: 14) McLaughlan JM, Anderson GH, Hackler LR, Hill DC, Jansen GR, Keith MO, Sarwar G, Sosulski FW (1980) Assessment of rat growth methods for estimating protein quality: Interlaboratory study. J Assoc Off Anal Chem 63: 462-7. – reference: 1) シルクサイエンス研究会編 (1994) シルクの科学, p. 7-9, p. 87-96, p. 113-23. 朝倉書店, 東京 – reference: 11) 弘田玲, 白鳥恭子, 中川滋木 (1998) 投与ペプチドの濃度変化による小腸内オルニチントランスカルバミラーゼ (OTC) への影響. 消化と吸収 21, 16-9. – reference: 4) 平尾和子, 塚越幸子, 五十嵐喜治 (1999) 絹フィブロイン起泡粉末の給与がラットの血清コレステロール濃度に及ぼす影響. 日本栄養・食糧学会誌 52, 219-23. – reference: 20) 平林潔, 陳開利, 勢籏毅 (1991) 絹フィブロインの食品素材としての活用. New Food Ind. 33: 1-4. – reference: 5) 平林潔 (1989) 絹の可溶化とその応用. Bio Ind. 6: 749-54. – reference: 17) Sugiyama K, Kushima Y, Muramatsu K (1985) Effect of sulfur-containing amino acid and glycine on plasma cholesterol level in rats fed on a high cholesterol diet. Agric Biol Chem 49: 3455-61. – reference: 21) 谷口宏吉, 冨田次男, 山止康弘 (1997) 絹蛋白質由来アンギオテンシンI変換酵素阻害ペプチドに関する研究. 明治大学農学部研究報告112, 1-19. – reference: 7) AOAC (1990) Official Methods of Analysis of the AOAC 15th Edition, p. 777-82. AOAC, Arlington. – reference: 9) 後藤逸男, 村本穣司, 蜷木翠 (1992) テフロン加圧分解容器-ICP発光分光分析法による植物の無機成分分析. 日本土壌肥料学雑誌 63, 345-8. – reference: 8) 後藤逸男, 蜷木翠 (1991) メタホウ酸リチウム溶融-ICP発光分光分析法による植物の無機成分分析. 日本土壌肥料学雑誌 62, 628-32. – reference: 16) Hsu HW, Vavak DL, Satterlee LD, Miller GA (1977) A multienzyme technique for estimating protein digestibility. J Food Sci 42: 1269-77. |
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| Snippet | Digestibility of water-soluble fibroin has long remained unknown. The present study thus aimed to clarify the digestibility of water-soluble fibroin extracted... |
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| Title | Digestibility of Water-soluble Fibroin Prepared from Silk Yarn |
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