Application of post-discharge region of atmospheric pressure argon and air plasma jet in the contamination control of Candida albicans biofilms
Introduction:Candida species are responsible for about 80% of hospital fungal infections. Non-thermal plasmas operated at atmospheric pressure are increasingly used as an alternative to existing antimicrobial strategy. This work investigates the action of post-discharge region of a non-thermal atmos...
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Published in | Research on biomedical engineering Vol. 31; no. 4; pp. 358 - 362 |
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Main Authors | , , , , , , , |
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Language | English Portuguese |
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Sociedade Brasileira de Engenharia Biomédica
01.12.2015
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Abstract | Introduction:Candida species are responsible for about 80% of hospital fungal infections. Non-thermal plasmas operated at atmospheric pressure are increasingly used as an alternative to existing antimicrobial strategy. This work investigates the action of post-discharge region of a non-thermal atmospheric plasma jet, generated by a gliding arc reactor, on biofilms of standard strain of Candida albicans grown on polyurethane substrate. Methods Samples were divided into three groups: (i) non-treated; (ii) treated with argon plasma, and (iii) treated with argon plus air plasma. Subsequently to plasma treatment, counting of colony-forming units (CFU/ml) and cell viability tests were performed. In addition, the surface morphology of the samples was evaluated by scanning electron microscopy (SEM) and optical profilometry (OP). Results Reduction in CFU/ml of 85% and 88.1% were observed in groups ii and iii, respectively. Cell viability after treatment also showed reduction of 33% in group ii and 8% in group iii, in comparison with group i (100%). The SEM images allow observation of the effect of plasma chemistry on biofilm structure, and OP images showed a reduction of its surface roughness, which suggests a possible loss of biofilm mass. Conclusion The treatment in post-discharge region and the chemistries of plasma jet tested in this work were effective in controlling Candida albicans biofilm contamination. Finally, it was evidenced that argon plus air plasma was the most efficient to reduce cell viability. |
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AbstractList | Introduction:Candida species are responsible for about 80% of hospital fungal infections. Non-thermal plasmas operated at atmospheric pressure are increasingly used as an alternative to existing antimicrobial strategy. This work investigates the action of post-discharge region of a non-thermal atmospheric plasma jet, generated by a gliding arc reactor, on biofilms of standard strain of Candida albicans grown on polyurethane substrate. Methods Samples were divided into three groups: (i) non-treated; (ii) treated with argon plasma, and (iii) treated with argon plus air plasma. Subsequently to plasma treatment, counting of colony-forming units (CFU/ml) and cell viability tests were performed. In addition, the surface morphology of the samples was evaluated by scanning electron microscopy (SEM) and optical profilometry (OP). Results Reduction in CFU/ml of 85% and 88.1% were observed in groups ii and iii, respectively. Cell viability after treatment also showed reduction of 33% in group ii and 8% in group iii, in comparison with group i (100%). The SEM images allow observation of the effect of plasma chemistry on biofilm structure, and OP images showed a reduction of its surface roughness, which suggests a possible loss of biofilm mass. Conclusion The treatment in post-discharge region and the chemistries of plasma jet tested in this work were effective in controlling Candida albicans biofilm contamination. Finally, it was evidenced that argon plus air plasma was the most efficient to reduce cell viability. |
Author | Sorge, Camila Di Paula Costa Santos, Thaisa Baesso Maciel, Homero Santiago Gonçalves, Polyana Alves Radi Pessoa, Rodrigo Sávio Brandão, Jhonatan Khouri, Sônia Doria, Anelise Cristina Osório Cesar |
AuthorAffiliation | Universidade do Vale do Paraíba Instituto Tecnológico de Aeronáutica |
AuthorAffiliation_xml | – name: Instituto Tecnológico de Aeronáutica – name: Universidade do Vale do Paraíba |
Author_xml | – sequence: 1 givenname: Anelise Cristina Osório Cesar surname: Doria fullname: Doria, Anelise Cristina Osório Cesar organization: Universidade do Vale do Paraíba, Brazil – sequence: 2 givenname: Camila Di Paula Costa surname: Sorge fullname: Sorge, Camila Di Paula Costa organization: Universidade do Vale do Paraíba, Brazil – sequence: 3 givenname: Thaisa Baesso surname: Santos fullname: Santos, Thaisa Baesso organization: Universidade do Vale do Paraíba, Brazil – sequence: 4 givenname: Jhonatan surname: Brandão fullname: Brandão, Jhonatan organization: Universidade do Vale do Paraíba, Brasil – sequence: 5 givenname: Polyana Alves Radi surname: Gonçalves fullname: Gonçalves, Polyana Alves Radi organization: Universidade do Vale do Paraíba, Brasil; Instituto Tecnológico de Aeronáutica, Brazil – sequence: 6 givenname: Homero Santiago surname: Maciel fullname: Maciel, Homero Santiago organization: Universidade do Vale do Paraíba, Brazil; Universidade do Vale do Paraíba, Brasil; Instituto Tecnológico de Aeronáutica, Brazil – sequence: 7 givenname: Sônia surname: Khouri fullname: Khouri, Sônia organization: Universidade do Vale do Paraíba, Brazil; Universidade do Vale do Paraíba, Brazil – sequence: 8 givenname: Rodrigo Sávio surname: Pessoa fullname: Pessoa, Rodrigo Sávio organization: Universidade do Vale do Paraíba, Brazil; Universidade do Vale do Paraíba, Brasil; Instituto Tecnológico de Aeronáutica, Brazil |
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CitedBy_id | crossref_primary_10_1007_s42600_021_00179_8 crossref_primary_10_1007_s11090_023_10380_5 crossref_primary_10_1021_acsomega_8b03640 crossref_primary_10_1111_jam_13404 crossref_primary_10_1016_j_apsusc_2017_05_254 crossref_primary_10_3390_app11051975 crossref_primary_10_1088_2516_1067_aae7e1 crossref_primary_10_3390_biomedicines11051401 crossref_primary_10_3390_ijms24021606 |
Cites_doi | 10.1371/journal.pone.0044289 10.1007/s11046-013-9616-5 10.1016/j.ijantimicag.2014.01.025 10.1080/08927014.2014.995643 10.1109/TPS.2008.917165 10.1590/0103-6440201300052 10.1007/s11090-014-9545-1 10.1016/j.ijms.2003.11.016 10.1590/S0102-86502004000100009 10.1002/ppap.201400074 10.1109/TPS.2006.878381 10.1109/TPS.2002.804220 |
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Keywords | Candida albicans Biofilm Cell viability Gliding arc plasma |
Language | English Portuguese |
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References | Ruiz LS (ref9) 2013; 175 Ma Y (ref7) 2008; 36 Laroussi M (ref6) 2004; 233 Alkawareek MY (ref1) 2012; 7 Chen C (ref2) 2014; 34 Laroussi M (ref5) 2002; 30 Saad-Hossne R (ref10) 2004; 19 Kong MG (ref4) 2009; 11 Taghizadeh L (ref11) 2015; 12 Traba C (ref12) 2015; 31 Gaunt LF (ref3) 2006; 34 Vasconcelos LC (ref13) 2014; 25 Mai-Prochnow A (ref8) 2014; 43 2004; 233 2009; 11 2015; 12 2006; 34 2002; 30 2004; 19 2015; 31 2008; 36 2014; 25 2013; 175 2012; 7 2014; 34 2014; 43 |
References_xml | – volume: 7 start-page: e44289 issue: 8 year: 2012 ident: ref1 article-title: Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma publication-title: PloS One. doi: 10.1371/journal.pone.0044289 contributor: fullname: Alkawareek MY – volume: 175 start-page: 231 issue: 3-4 year: 2013 ident: ref9 article-title: Candidemia by species of the Candida parapsilosis complex in children’s hospital: prevalence, biofilm production and antifungal susceptibility publication-title: Mycopathologia doi: 10.1007/s11046-013-9616-5 contributor: fullname: Ruiz LS – volume: 11 start-page: 1 issue: 11 year: 2009 ident: ref4 article-title: Plasma medicine: an introductory review publication-title: New Journal of Physics contributor: fullname: Kong MG – volume: 43 start-page: 508 issue: 6 year: 2014 ident: ref8 article-title: Atmospheric pressure plasmas: infection control and bacterial responses publication-title: International Journal of Antimicrobial Agents doi: 10.1016/j.ijantimicag.2014.01.025 contributor: fullname: Mai-Prochnow A – volume: 31 start-page: 39 issue: 1 year: 2015 ident: ref12 article-title: The inactivation of Staphylococcus aureus biofilms using low-power argon plasma in a layer-by-layer approach publication-title: Biofouling doi: 10.1080/08927014.2014.995643 contributor: fullname: Traba C – volume: 36 start-page: 1615 issue: 4 year: 2008 ident: ref7 article-title: Chemical mechanisms of bacterial inactivation using dielectric barrier discharge plasma in atmospheric air publication-title: IEEE Transactions on Plasma Science doi: 10.1109/TPS.2008.917165 contributor: fullname: Ma Y – volume: 25 start-page: 277 issue: 4 year: 2014 ident: ref13 article-title: Cell viability of Candida albicans against the antifungal activity of thymol publication-title: Brazilian Dental Journal doi: 10.1590/0103-6440201300052 contributor: fullname: Vasconcelos LC – volume: 34 start-page: 403 issue: 3 year: 2014 ident: ref2 article-title: A model of plasma-biofilm and plasma-tissue interactions at ambient pressure publication-title: Plasma Chemistry and Plasma Processing doi: 10.1007/s11090-014-9545-1 contributor: fullname: Chen C – volume: 233 start-page: 81 issue: 1-3 year: 2004 ident: ref6 article-title: Evaluation of the roles of reactive species, heat, and uv radiation in the inactivation of bacterial cells by air plasmas at atmospheric pressure publication-title: International Journal of Mass Spectrometry doi: 10.1016/j.ijms.2003.11.016 contributor: fullname: Laroussi M – volume: 19 start-page: 54 issue: 1 year: 2004 ident: ref10 article-title: Efeito da solução aquosa de fenol, ácido acético e glicerina sobre o tumor ascítico de Ehrlich. Estudo experimental in vitro publication-title: Acta Cirurgica Brasileira doi: 10.1590/S0102-86502004000100009 contributor: fullname: Saad-Hossne R – volume: 12 start-page: 75 issue: 1 year: 2015 ident: ref11 article-title: Inactivation of biofilms using a low power atmospheric pressure argon plasma jet; the role of entrained nitrogen publication-title: Plasma Processes and Polymers doi: 10.1002/ppap.201400074 contributor: fullname: Taghizadeh L – volume: 34 start-page: 1257 issue: 4 year: 2006 ident: ref3 article-title: Bactericidal action of the reactive species produced by gas-discharge nonthermal plasma at atmospheric pressure: a review publication-title: IEEE Transactions on Plasma Science doi: 10.1109/TPS.2006.878381 contributor: fullname: Gaunt LF – volume: 30 start-page: 1409 issue: 4 year: 2002 ident: ref5 article-title: Nonthermal decontamination of biological media by atmospheric-pressure plasmas: review, analysis, and prospects publication-title: IEEE Transactions on Plasma Science doi: 10.1109/TPS.2002.804220 contributor: fullname: Laroussi M – volume: 12 start-page: 75 issue: 1 year: 2015 end-page: 81 article-title: Inactivation of biofilms using a low power atmospheric pressure argon plasma jet; the role of entrained nitrogen publication-title: Plasma Processes and Polymers – volume: 34 start-page: 403 issue: 3 year: 2014 end-page: 441 article-title: A model of plasma-biofilm and plasma-tissue interactions at ambient pressure publication-title: Plasma Chemistry and Plasma Processing – volume: 175 start-page: 231 issue: 3-4 year: 2013 end-page: 239 article-title: Candidemia by species of the Candida parapsilosis complex in children’s hospital: prevalence, biofilm production and antifungal susceptibility publication-title: Mycopathologia – volume: 31 start-page: 39 issue: 1 year: 2015 end-page: 48 article-title: The inactivation of Staphylococcus aureus biofilms using low-power argon plasma in a layer-by-layer approach publication-title: Biofouling – volume: 34 start-page: 1257 issue: 4 year: 2006 end-page: 1269 article-title: Bactericidal action of the reactive species produced by gas-discharge nonthermal plasma at atmospheric pressure: a review publication-title: IEEE Transactions on Plasma Science – volume: 7 start-page: e44289 issue: 8 year: 2012 article-title: Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma publication-title: PloS One. – volume: 36 start-page: 1615 issue: 4 year: 2008 end-page: 1620 article-title: Chemical mechanisms of bacterial inactivation using dielectric barrier discharge plasma in atmospheric air publication-title: IEEE Transactions on Plasma Science – volume: 233 start-page: 81 issue: 1-3 year: 2004 end-page: 86 article-title: Evaluation of the roles of reactive species, heat, and uv radiation in the inactivation of bacterial cells by air plasmas at atmospheric pressure publication-title: International Journal of Mass Spectrometry – volume: 19 start-page: 54 issue: 1 year: 2004 end-page: 58 article-title: Efeito da solução aquosa de fenol, ácido acético e glicerina sobre o tumor ascítico de Ehrlich. Estudo experimental in vitro publication-title: Acta Cirurgica Brasileira – volume: 43 start-page: 508 issue: 6 year: 2014 end-page: 517 article-title: Atmospheric pressure plasmas: infection control and bacterial responses publication-title: International Journal of Antimicrobial Agents – volume: 30 start-page: 1409 issue: 4 year: 2002 end-page: 1415 article-title: Nonthermal decontamination of biological media by atmospheric-pressure plasmas: review, analysis, and prospects publication-title: IEEE Transactions on Plasma Science – volume: 11 start-page: 1 issue: 11 year: 2009 end-page: 35 article-title: Plasma medicine: an introductory review publication-title: New Journal of Physics – volume: 25 start-page: 277 issue: 4 year: 2014 end-page: 281 article-title: Cell viability of Candida albicans against the antifungal activity of thymol publication-title: Brazilian Dental Journal |
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Title | Application of post-discharge region of atmospheric pressure argon and air plasma jet in the contamination control of Candida albicans biofilms |
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