Probing the interaction of solvents with the stationary phase of C18 high-performance liquid chromatographic column material by variable-temperature dependent 129Xe nuclear magnetic resonance spectroscopy

• Published in: Journal of Chromatography A Vol. 1121; no. 1; pp. 23 - 31
• Main Authors: Chagolla, Danny, Mathias, Errol V., Ba, Yong
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 14.07.2006; Elsevier
• Subjects: 129Xe NMR; Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - instrumentation; Exact sciences and technology; HPLC column material; Interaction of stationary phase and solvents; Magnetic Resonance Spectroscopy - methods; Other chromatographic methods; Silicon Dioxide - chemistry; Temperature; Interaction of stationary phase and solvents; 129Xe NMR; HPLC column material; Solvent effect; Reversed phase chromatography; HPLC chromatography; Xenon 129; NMR spectrometry; Mobile phase; Bonded stationary phase

VT 129Xe NMR was applied to probe the interactions of solvents having different polarity indices with the stationary phase of a RP-C18 HPLC column material. It was observed that the highly polar ethylene glycol molecules do not mix with the alkyl chains of the RP-C18 stationary phase and the solvent...