Unveiling of active diazotrophs in a flooded rice soil by combination of NanoSIMS and 15N2-DNA-stable isotope probing

• Published in: Biology and fertility of soils Vol. 56; no. 8; pp. 1189 - 1199
• Main Authors: Wang, Xiaojie, Bei, Qicheng, Yang, Wei, Zhang, Heng, Hao, Jialong, Qian, Li, Feng, Yucheng, Xie, Zubin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2020
• Subjects: Agriculture; Biomedical and Life Sciences; community structure; DNA; Life Sciences; mass spectrometry; nitrogen fixation; nitrogen-fixing bacteria; Nostocales; Original Paper; paddies; rice; rice soils; Soil Science & Conservation; Stigonematales; ultracentrifugation; NanoSIMS; Rice soil; labeling; N; Cyanobacteria; DNA-SIP

The biological nitrogen fixation in planted and nonplanted paddy soils was quantified using a chamber-based 15 N 2 -labeling technique, and the active diazotrophs of soil were assessed by 15 N 2 -DNA-stable isotope probing (SIP). In addition, the nanometer scale secondary ion mass spectrometry (Nano...