MicroCommentary: Smarter than the average phage
Summary The seventh cholera pandemic emerged in the poorer nations of the world towards the end of the 20th century and continues to kill thousands of people per year. The causative agent of cholera, the Gram‐negative bacterium Vibrio cholera, is only pathogenic when it contains a lysogenic bacterio...
Saved in:
Published in | Molecular microbiology Vol. 54; no. 4; pp. 851 - 854 |
---|---|
Main Author | |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Science Ltd
01.11.2004
Blackwell Publishing Ltd |
Online Access | Get full text |
Cover
Loading…
Abstract | Summary
The seventh cholera pandemic emerged in the poorer nations of the world towards the end of the 20th century and continues to kill thousands of people per year. The causative agent of cholera, the Gram‐negative bacterium Vibrio cholera, is only pathogenic when it contains a lysogenic bacteriophage, CTXφ, that encodes the toxin responsible for inducing massive fluid loss from the human host. Site‐specific integration of CTXφ into chromosome I of V. cholera occurs at a site, dif, that is normally required for resolution of chromosome dimers generated by homologous recombination. An article in this issue of Molecular Microbiology reports the analysis of interactions between two host encoded recombinases, XerC and XerD, and the recombination sites involved in lysogeny. Surprisingly, recombination between the CTXφattP site and the chromosomal dif site requires additional recombinase binding sites, downstream from the positions of strand exchange, which might play an architectural role. The positions of strand cleavage also differ significantly between the two sites, suggesting a novel recombination mechanism that implicates additional host factors in resolution of the Holliday junction intermediate. |
---|---|
AbstractList | Summary
The seventh cholera pandemic emerged in the poorer nations of the world towards the end of the 20th century and continues to kill thousands of people per year. The causative agent of cholera, the Gram‐negative bacterium Vibrio cholera, is only pathogenic when it contains a lysogenic bacteriophage, CTXφ, that encodes the toxin responsible for inducing massive fluid loss from the human host. Site‐specific integration of CTXφ into chromosome I of V. cholera occurs at a site, dif, that is normally required for resolution of chromosome dimers generated by homologous recombination. An article in this issue of Molecular Microbiology reports the analysis of interactions between two host encoded recombinases, XerC and XerD, and the recombination sites involved in lysogeny. Surprisingly, recombination between the CTXφattP site and the chromosomal dif site requires additional recombinase binding sites, downstream from the positions of strand exchange, which might play an architectural role. The positions of strand cleavage also differ significantly between the two sites, suggesting a novel recombination mechanism that implicates additional host factors in resolution of the Holliday junction intermediate. The seventh cholera pandemic emerged in the poorer nations of the world towards the end of the 20th century and continues to kill thousands of people per year. The causative agent of cholera, the Gram‐negative bacterium Vibrio cholera , is only pathogenic when it contains a lysogenic bacteriophage, CTXφ, that encodes the toxin responsible for inducing massive fluid loss from the human host. Site‐specific integration of CTXφ into chromosome I of V. cholera occurs at a site, dif , that is normally required for resolution of chromosome dimers generated by homologous recombination. An article in this issue of Molecular Microbiology reports the analysis of interactions between two host encoded recombinases, XerC and XerD, and the recombination sites involved in lysogeny. Surprisingly, recombination between the CTXφ attP site and the chromosomal dif site requires additional recombinase binding sites, downstream from the positions of strand exchange, which might play an architectural role. The positions of strand cleavage also differ significantly between the two sites, suggesting a novel recombination mechanism that implicates additional host factors in resolution of the Holliday junction intermediate. |
Author | Blakely, Garry W. |
Author_xml | – sequence: 1 givenname: Garry W. surname: Blakely fullname: Blakely, Garry W. |
BookMark | eNqNkE1PwzAMhiM0JLrBf6i4t0viJG2QOKCJj0mbOAAStygrDlu1tiPtYP33pAxxxgfbkv364xmTUd3USEjMaMqCTcuUgZIJ1zJPOaUipQKApocTEv0VRiSiWtIEcv56RsZtW1LKgCqIyHS5KXwza6oK6876_ip-qqzv0Mfd2tbBYWw_0dt3jHfr4M_JqbPbFi9-44S83N0-zx6SxeP9fHazSAoOYZMF6rTO8hVH0CxzwKXgkqsiY8Wbk1iwIhcKALnSmoHOnLDMMZ6tcglWSZiQy-PcnW8-9th2pmz2vg4rDdNKcgFShab82BReaFuPzuz8JpzfG0bNQMeUZoBgBghmoGN-6JhDkF4fpV-bLfb_1pnlcj5k8A0fpmoq |
CitedBy_id | crossref_primary_10_1128_MMBR_00011_11 crossref_primary_10_1186_1471_2164_12_19 crossref_primary_10_1016_j_molcel_2005_07_002 |
Cites_doi | 10.1016/0092-8674(93)80076-Q 10.1074/jbc.270.8.4042 10.1038/nature00782 10.1016/S0092-8674(02)00624-4 10.1098/rstb.1995.0006 |
ContentType | Journal Article |
Copyright | Copyright Blackwell Scientific Publications Ltd. Nov 2004 |
Copyright_xml | – notice: Copyright Blackwell Scientific Publications Ltd. Nov 2004 |
DBID | AAYXX CITATION 7QL 7QP 7QR 7TK 7TM 7U9 8FD C1K FR3 H94 M7N P64 RC3 |
DOI | 10.1111/j.1365-2958.2004.04330.x |
DatabaseName | CrossRef Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database AIDS and Cancer Research Abstracts Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts Genetics Abstracts |
DatabaseTitle | CrossRef Virology and AIDS Abstracts Technology Research Database Nucleic Acids Abstracts Neurosciences Abstracts Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management Genetics Abstracts Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts |
DatabaseTitleList | CrossRef |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 1365-2958 |
EndPage | 854 |
ExternalDocumentID | 742186321 10_1111_j_1365_2958_2004_04330_x MMI4330 |
Genre | commentary |
GroupedDBID | --- -DZ .3N .55 .GA .GJ .HR .Y3 05W 0R~ 10A 123 1OB 1OC 24P 29M 2WC 31~ 33P 36B 3SF 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52U 52W 52X 53G 5HH 5LA 5RE 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A03 AAESR AAEVG AAHHS AAKAS AANLZ AAONW AASGY AAXRX AAZKR ABCQN ABCUV ABEML ABJNI ABPVW ABTAH ACAHQ ACBWZ ACCFJ ACCZN ACFBH ACGFO ACGFS ACIWK ACNCT ACPOU ACPRK ACSCC ACXBN ACXQS ADBBV ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZCM ADZMN AEEZP AEGXH AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFEBI AFFPM AFGKR AFPWT AFRAH AFZJQ AHBTC AHEFC AIAGR AITYG AIURR AIWBW AJBDE AJXKR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ASPBG ATUGU AUFTA AVWKF AZBYB AZFZN AZVAB BAFTC BAWUL BDRZF BFHJK BHBCM BMNLL BMXJE BNHUX BROTX BRXPI BY8 C45 CAG COF D-E D-F DC6 DCZOG DIK DPXWK DR2 DRFUL DRSTM E3Z EBS EJD EMOBN ESX EX3 F00 F01 F04 F5P FEDTE FIJ FSRTE FZ0 G-S G.N GODZA GX1 H.T H.X HF~ HGLYW HH5 HVGLF HZI HZ~ IH2 IHE IPNFZ IX1 J0M K48 LATKE LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRSTM MSFUL MSSTM MVM MXFUL MXSTM N04 N05 N9A NF~ O66 O9- OBC OBS OEB OIG OK1 OVD P2P P2W P2X P4D PALCI PQQKQ Q.N Q11 QB0 R.K RIWAO RJQFR ROL RX1 SAMSI SUPJJ TEORI TR2 UB1 V8K W8V W99 WBKPD WH7 WIH WIK WIN WNSPC WOHZO WOW WQJ WRC WUP WXSBR WYISQ X7M XG1 Y6R YFH YUY ZGI ZXP ZY4 ZZTAW ~IA ~KM ~WT AAMNL AAYXX ACRPL CITATION 7QL 7QP 7QR 7TK 7TM 7U9 8FD C1K FR3 H94 M7N P64 RC3 |
ID | FETCH-LOGICAL-c2350-a30f9978b2e3917f32542526c71cdf5ec1c84633e26991397f4a1f127b853a653 |
IEDL.DBID | DR2 |
ISSN | 0950-382X |
IngestDate | Thu Oct 10 16:52:15 EDT 2024 Fri Dec 06 03:58:34 EST 2024 Sat Aug 24 01:01:46 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Language | English |
License | http://onlinelibrary.wiley.com/termsAndConditions#vor |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c2350-a30f9978b2e3917f32542526c71cdf5ec1c84633e26991397f4a1f127b853a653 |
OpenAccessLink | https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/j.1365-2958.2004.04330.x |
PQID | 196524356 |
PQPubID | 35968 |
PageCount | 4 |
ParticipantIDs | proquest_journals_196524356 crossref_primary_10_1111_j_1365_2958_2004_04330_x wiley_primary_10_1111_j_1365_2958_2004_04330_x_MMI4330 |
PublicationCentury | 2000 |
PublicationDate | November 2004 2004-11-00 20041101 |
PublicationDateYYYYMMDD | 2004-11-01 |
PublicationDate_xml | – month: 11 year: 2004 text: November 2004 |
PublicationDecade | 2000 |
PublicationPlace | Oxford, UK |
PublicationPlace_xml | – name: Oxford, UK – name: Oxford |
PublicationTitle | Molecular microbiology |
PublicationYear | 2004 |
Publisher | Blackwell Science Ltd Blackwell Publishing Ltd |
Publisher_xml | – name: Blackwell Science Ltd – name: Blackwell Publishing Ltd |
References | 1995; 347 1995; 270 2002; 108 1993; 75 2002; 417 e_1_2_2_4_1 e_1_2_2_5_1 e_1_2_2_6_1 e_1_2_2_2_1 e_1_2_2_3_1 |
References_xml | – volume: 75 start-page: 351 year: 1993 end-page: 361 article-title: Two related recombinases are required for site‐specific recombination at and in K12 publication-title: Cell – volume: 270 start-page: 4042 year: 1995 end-page: 4052 article-title: Role of partner homology in DNA recombination. Complementary base pairing orients the 5′‐hydroxyl for strand joining during Flp site‐specific recombination publication-title: J Biol Chem – volume: 108 start-page: 195 year: 2002 end-page: 205 article-title: FtsK is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases publication-title: Cell – volume: 347 start-page: 37 year: 1995 end-page: 42 article-title: Site‐specific recombination and circular chromosome segregation publication-title: Philos Trans R Soc Lond B Biol Sci – volume: 417 start-page: 656 year: 2002 end-page: 659 article-title: Filamentous phage integration requires the host recombinases XerC and XerD publication-title: Nature – ident: e_1_2_2_3_1 doi: 10.1016/0092-8674(93)80076-Q – ident: e_1_2_2_5_1 doi: 10.1074/jbc.270.8.4042 – ident: e_1_2_2_4_1 doi: 10.1038/nature00782 – ident: e_1_2_2_2_1 doi: 10.1016/S0092-8674(02)00624-4 – ident: e_1_2_2_6_1 doi: 10.1098/rstb.1995.0006 |
SSID | ssj0013063 |
Score | 1.7926669 |
Snippet | Summary
The seventh cholera pandemic emerged in the poorer nations of the world towards the end of the 20th century and continues to kill thousands of people... The seventh cholera pandemic emerged in the poorer nations of the world towards the end of the 20th century and continues to kill thousands of people per year.... |
SourceID | proquest crossref wiley |
SourceType | Aggregation Database Publisher |
StartPage | 851 |
Title | MicroCommentary: Smarter than the average phage |
URI | https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1365-2958.2004.04330.x https://www.proquest.com/docview/196524356 |
Volume | 54 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3PS8MwFA4yELz4W5xT6cFra5O0aeNN1DmFelAHvYU0SxGGc-wHOP9630s7t4kHES-PXhra17zke69fvkfImej1TGK08E0hIz-SOvYl08aH3V1ogK_alnjAOXsQnW50n8d5zX_CszCVPsRXwQ0jw63XGOC6GK8GuWNoyTh1aV6AUlxhgHiS8gTZfdePbPFDoW6qJmOUk2X5Kqnnx4FWd6oF_FwGsW4Xam-R_vz5K_JJP5hOisB8fJN2_J8X3CabNVj1LqvZtUPW7GCXrFftK2d75DxDNh8eMUEC-mh24T29Ooqoh_V4MNbTECmwYnnDF7D7pNu-eb7q-HUDBt8wDo7SPCwlpJkFsxzSupJDNsliJkxCTa-MraEG4AvnlgmJ8qJJGWlaUpYUAAK0iPkBaQzeBvaQeIaG0hpU-0sh4xEm5TqNUgRHrOxFoW0SOne2GlY6G2opPwFHKHQEds2MlHOEem-S1vyrqDryxgoVEhlgQNEkwnn318OpLLvDq6O_3tgiG5UMJBZojkljMpraE4Ask-LUTUawtzn9BHlU2pg |
link.rule.ids | 314,780,784,1375,27924,27925,46294,46718 |
linkProvider | Wiley-Blackwell |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV09T8MwELUQCMHCN6KUjwysCYmdODEbAqoWmg7QSt0sx3WEBJSqtBLl13PnpLRFDAixWFliyRef793l-R0hZ7zX07FW3NWZCN1QqMgVVGkXojtXAF-VyfGCc9ri9U542426ZTsgvAtT6EN8FdzQM-x5jQ6OBelFL7cULRElNs_zUIvL9wBQroD3B8jvur6ns18KZVs1EaGgLO0u0np-nGkxVs0A6DyMtXGotkmepyso6CdP3niUefrjm7jjPy1xi2yUeNW5LDbYNlky_R2yWnSwnOyS8xQJfXjLBDnow8mF8_BiWaIOluRhMI4CZ4FDyxk8wrhHOrWb9lXdLXswuJoysJRifi4g08yoYZDZ5QwSShpRruNA9_LI6EADgmHMUC5QYTTOQxXkAY0zwAGKR2yfLPdf--aAODrwhdEo-JdA0sN1wlQSJoiPaN4LfVMhwdTaclBIbci5FAUMIdEQ2DgzlNYQ8r1CqtPPIkvne5MokkgBBvIK4da8v55OpmkDnw7_-uIpWau306ZsNlp3VbJeqEJiveaILI-GY3MMCGaUndid-Qk3gd3I |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3PS8MwFA4yUbz4W5zzRw9eW9ekTRtv4hyb2iHqYLeQpinCcI65gfOv97200008iHgJvTSQ17y876Xf-x4hpzzLdKQVd3UqAjcQKnQFVdqF6M4VwFdlcixwTjq81Q2ue2Gv5D9hLUyhD_F54YaeYc9rdPBhli86uWVoiTC2aZ6HUlx1D_DkcsCpQB39xj39-qNQdlUTIerJ0t4iq-fHmRZD1Rf-nEexNgw1N0h_toCCfdL3JuPU0-_ftB3_Z4WbZL1Eq85Fsb22yJIZbJOVon_ldIecJUjnwxoTZKCPpufOw7PliDp4IQ-DcRS4ChxZzvAJxl3SbV49XrbcsgODqykDQylWzwXkmSk1DPK6nEE6SUPKdeTrLA-N9jXgF8YM5QL1RaM8UH7u0ygFFKB4yPZIZfAyMPvE0X5dGI1yfzGkPFzHTMVBjOiI5llQN1Xiz4wth4XQhpxLUMAQEg2BbTMDaQ0h36qkNvsqsnS9V4kSiRRAIK8Sbq376-lkkrTx6eCvL56Q1btGU962Ozc1slZIQuJlzSGpjEcTcwTwZZwe2335AaCB3Hc |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=MicroCommentary%3A+Smarter+than+the+average+phage&rft.jtitle=Molecular+microbiology&rft.au=Blakely%2C+Garry+W.&rft.date=2004-11-01&rft.pub=Blackwell+Science+Ltd&rft.issn=0950-382X&rft.eissn=1365-2958&rft.volume=54&rft.issue=4&rft.spage=851&rft.epage=854&rft_id=info:doi/10.1111%2Fj.1365-2958.2004.04330.x&rft.externalDBID=10.1111%252Fj.1365-2958.2004.04330.x&rft.externalDocID=MMI4330 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0950-382X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0950-382X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0950-382X&client=summon |