Positional scanning substrate combinatorial library (PS-SCL) approach to define caspase substrate specificity

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1133; p. 41
• Main Authors: Poręba, Marcin, Szalek, Aleksandra, Kasperkiewicz, Paulina, Drąg, Marcin
• Format: Journal Article
• Language: English
• Published: United States 2014
• Subjects: Amino Acid Sequence; Caspases - chemistry; Caspases - genetics; Caspases - metabolism; Combinatorial Chemistry Techniques; Humans; Kinetics; Molecular Biology - methods; Peptide Library; Substrate Specificity

Positional scanning substrate combinatorial library (PS-SCL) is a powerful tool for studying substrate specificity of proteolytic enzymes. Here, we describe the protocol for analyzing S4-S2 pockets preferences of caspases using PS-SCL. Additionally, we describe procedures for the identification of o...