Use of mini-antibodies for detection of bacteriophages by the electroaucoustic analysis method

• Published in: Biophysics (Oxford) Vol. 62; no. 3; pp. 373 - 384
• Main Authors: Guliy, O. I., Zaitsev, B. D., Borodina, I. A., Fomin, A. S., Staroverov, S. A., Dykman, L. A., Shikhabudinov, A. M.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.05.2017; Springer Nature B.V
• Subjects: Antibodies; Biological and Medical Physics; Biophysics; Electrical impedance; Frequency dependence; Immunoglobulins; Impedance; Mini; Molecular Biophysics; Phage; Phages; Physics; Physics and Astronomy; Sensors; Viruses; electroacoustic analysis method; piezoelectric lateral electric field-excited resonator; phage mini-antibodies; bacteriophages FAl-Sp59b; Azospirillum lipoferum Sp59b; bacteriophages
• ISSN: 0006-3509; 1555-6654
• DOI: 10.1134/S000635091703006X

The possibility of detecting bacteriophages using phage mini-antibodies by the electroacoustic analysis method using bacteriophages FA1-59b was shown. It was found that the frequency dependence of the real and imaginary parts of the electrical impedance of a resonator with a suspension of phages and the appropriate antibodies significantly differs from that of the resonator with a control virus suspension without addition of mini-antibodies. The amount of FAl-Sp59b bacteriophage in the analyzed suspension varied from ~10 10 to 10 6 phage/mL; the analysis did not take longer than 5 min. The change in the real or imaginary parts of the electrical impedance at the fixed frequency near the resonance after addition of specific mini-antibodies in the suspension appeared to be an optimal information parameter to obtain reliable information. These results may allow the development of a biological sensor to identify and quantify viruses in the liquid phase.