Effect of 21-Day Dry Immersion on Expression of Inborn Immunity Genes Associated with the Toll-Like Receptors of Signaling Pathways

• Published in: Human physiology Vol. 49; no. 7; pp. 807 - 814
• Main Authors: Vlasova, D. D., Sadova, A. A., Galina, V. S., Guermanov, N. S., Rykova, M. P., Antropova, E. N., Kutko, O. V., Shulguina, S. M., Orlova, K. D., Shmarov, V. A., Lysenko, E. A., Ponomarev, S. A.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.12.2023; Springer Nature B.V
• Subjects: AKT protein; Apoptosis; Biomedical and Life Sciences; Biomedicine; CD14 antigen; Chloroform; Experimental and General Theoretical Studies; Human Physiology; Humoral immunity; Immersion; Immune response; Immunity; Life Sciences; Monocytes; NF-κB protein; Peripheral blood; Receptor mechanisms; TLR4 protein; Toll-like receptors; Transcription factors; Toll-like receptors; dry immersion; image-recognizing receptors; inborn immunity; gene expression
• ISSN: 0362-1197; 1608-3164
• DOI: 10.1134/S0362119723070174

Expression of inborn immunity genes was studied in 10 essentially healthy male subjects aged 24 to 32 years in a 21-day dry immersion (DI) experiment without countermeasures. To evaluate the humoral immunity function, we selected genes encoding Toll-like receptors; proteins mediating NF-kB, AP-1, Akt, and TLR4 pathways; apoptosis proteins; CLEC4E , HSPD1 , and PTGS2 genes. Phenol-chloroform extraction was used to obtain total RNA from peripheral blood monocytes CD14+. Subsequently, total RNA was reverse transcribed to generate cDNA for real-time PCR. The results showed substantial changes in expression of these genes suggesting that changes in the TLR function on the transcriptional level are observed from the earliest stages of the immune response to 21-d DI.