Substrate specificity, physicochemical and kinetic properties of a trypsin from the giant Amazonian fish pirarucu (Arapaima gigas)

• Published in: Biocatalysis and agricultural biotechnology Vol. 35; p. 102073
• Main Authors: de Freitas-Júnior, Augusto Cézar V., da Costa, Helane Maria S., Marcuschi, Marina, Icimoto, Marcelo Y., Machado, Marcelo F.M., Machado, Maurício F.M., Ferreira, Juliana C., de Oliveira, Vitor M.S.B.B., Buarque, Diego S., Bezerra, Ranilson S.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.08.2021
• Subjects: Calcium activation; Fish enzymes; Serine proteases; Substrate specificity; Fish enzymes; Serine proteases; Calcium activation; Substrate specificity

Specificity studies can contribute to the understanding of how substrate binding and catalysis change among similar enzymes. This study evaluated through the substrate specificity, physicochemical and kinetic properties of a trypsin from the pyloric caeca of pirarucu (Arapaima gigas). For such purpo...