The crystal structure of phenylpyruvate decarboxylase from Azospirillum brasilense at 1.5 Å resolution Implications for its catalytic and regulatory mechanism

• Published in: The FEBS journal Vol. 274; no. 9; pp. 2363 - 2375
• Main Authors: Versées, Wim, Spaepen, Stijn, Vanderleyden, Jos, Steyaert, Jan
• Format: Journal Article
• Language: English
• Published: England 01.05.2007
• Subjects: Allosteric Site - genetics; Amino Acid Substitution - genetics; Azospirillum brasilense - enzymology; Azospirillum brasilense - genetics; Carboxy-Lyases - chemistry; Carboxy-Lyases - genetics; Carboxy-Lyases - metabolism; Catalysis; Cloning, Molecular; Crystallization; Crystallography, X-Ray; Dimerization; Enzyme Activation - genetics; Glutamic Acid - genetics; Leucine - genetics; Models, Chemical; Models, Molecular; Substrate Specificity - genetics
• ISSN: 1742-464X; 1742-4658
• DOI: 10.1111/j.1742-4658.2007.05771.x

Phenylpyruvate decarboxylase (PPDC) of Azospirillum brasilense , involved in the biosynthesis of the plant hormone indole‐3‐acetic acid and the antimicrobial compound phenylacetic acid, is a thiamine diphosphate‐dependent enzyme that catalyses the nonoxidative decarboxylation of indole‐ and phenylpy...