Development of a PCR Method to Detect a Mysid Species Causing False Positives in Tests for Crustacean Allergens

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 65; no. 3; pp. 48 - 52
• Main Authors: Hosokawa, Aoi, Sugano, Yohei, Aotuka, Keiji, Suzuki, Tomohiro
• Format: Journal Article
• Language: English; Japanese
• Published: Japan Japanese Society for Food Hygiene and Safety 2024; Japan Science and Technology Agency
• Subjects: Allergens; Allergens - analysis; Animals; Anomura - genetics; Aquatic crustaceans; Body organs; Crabs; Crustacea; Crustaceans; Decapoda; Deoxyribonucleic acid; DNA; DNA - analysis; DNA sequencing; Enzyme-Linked Immunosorbent Assay - methods; false positive; False Positive Reactions; Food; Food allergies; Food Analysis - methods; Food consumption; Food contamination; Food Contamination - analysis; Food Hypersensitivity; Food processing; Foods; Freshwater crustaceans; Gene sequencing; Japanese smelts; Krill; Marine crustaceans; mysid species; Nucleotide sequence; Organs; PCR; PCR polymerase chain reaction; Polymerase chain reaction; Polymerase Chain Reaction - methods; Prawns; Processed foods; Raw materials; shrimp/prawn and crab allergy; Shrimps; Japanese smelts; mysid species; PCR polymerase chain reaction; false positive; shrimp/prawn and crab allergy

Mysids are small crustaceans that are closely related to shrimp/prawns and crabs but not subject to food allergen labeling requirements for raw materials. In the past, a processed food that contained Japanese smelt (wakasagi) was suspected of producing a false-positive result in shrimp/prawn and cra...