Metabolic Profiling of Human Colorectal Cancer Using High Resolution 1H Nuclear Magnetic Resonance Spectroscopy

Colorectal cancer （CRC） is the third commonest malignancy cancer worldwide. Clear understandings of global metabolic profiling of the normal mucosa and cancer tissues are vitally important to aid optimizing the clinical management strategy and understanding CRC biology. We studied metabolic characte...

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Published inChinese journal of chemistry Vol. 29; no. 11; pp. 2511 - 2519
Main Author 陈文学 周晓燕 黄丹 陈芬儿 杜祥
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 01.11.2011
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
Subjects
Colorectal cancer
Colorectal carcinoma
metabolic profiling
Metabolism
Metabolites
multivariate analyses
NMR
NMR spectroscopy
Nuclear magnetic resonance
orthogonal partial least squares-discriminant analysis (OPLS-DA)
Polyamines
principal component analysis (PCA)
Principal components analysis
Spectroscopy
Spectrum analysis
Tissues
人类
代谢特点
大肠癌
恶性肿瘤
核磁共振光谱
生物标志物
轮廓
高分辨率
Online AccessGet full text
ISSN1001-604X
1614-7065
DOI10.1002/cjoc.201180423

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Summary:Colorectal cancer （CRC） is the third commonest malignancy cancer worldwide. Clear understandings of global metabolic profiling of the normal mucosa and cancer tissues are vitally important to aid optimizing the clinical management strategy and understanding CRC biology. We studied metabolic characteristics of 20 CRC and 20 distant normal mucosa tissues extracts from 20 patients using high resolution ^1H NMR spectroscopy in conjunction with multivariate analyses, such as principal component analysis （PCA）. Compared with distant normal mucosa tissues, lactate, taurine, ornithine and polyamine were present at significantly higher levels in CRC tissue extracts whereas myo-inositol was present at significantly lower level. Two metabolites ratios such as myo-inositolltaurine and myo-inositol/（ornithine＋polyamine） appear to be the most valuable biomarkers for the differentiation CRC from normal mucosa tissues. Our data suggested that HR ~H NMR spectroscopy combined with multivariate analy- ses is a potentially useful technology for detecting malignant changes in the normal mucosa tissues, the technique may be further exploited for future CRC biomarker research or identification of targets for therapeutic manipulations.
Bibliography:31-1547/O6
colorectal cancer, NMR spectroscopy, metabolic profiling, multivariate analyses, principal component analysis （PCA）, orthogonal partial least squares-discriminant analysis （OPLS-DA）
Colorectal cancer （CRC） is the third commonest malignancy cancer worldwide. Clear understandings of global metabolic profiling of the normal mucosa and cancer tissues are vitally important to aid optimizing the clinical management strategy and understanding CRC biology. We studied metabolic characteristics of 20 CRC and 20 distant normal mucosa tissues extracts from 20 patients using high resolution ^1H NMR spectroscopy in conjunction with multivariate analyses, such as principal component analysis （PCA）. Compared with distant normal mucosa tissues, lactate, taurine, ornithine and polyamine were present at significantly higher levels in CRC tissue extracts whereas myo-inositol was present at significantly lower level. Two metabolites ratios such as myo-inositolltaurine and myo-inositol/（ornithine＋polyamine） appear to be the most valuable biomarkers for the differentiation CRC from normal mucosa tissues. Our data suggested that HR ~H NMR spectroscopy combined with multivariate analy- ses is a potentially useful technology for detecting malignant changes in the normal mucosa tissues, the technique may be further exploited for future CRC biomarker research or identification of targets for therapeutic manipulations.
Chen, Wenxue Zhou, Xiaoyan Huang, Dan Chen, Fener Du, Xiang（a Department of Chemistry, Fudan University, Shanghai 200433, China b Department of Pathology and Oncology, Cancer Hospital, Fudan University, Shanghai 200032, China）
ArticleID:CJOC201180423
the National Natural Science Foundation of China - No. 20872018
istex:07373739884F53BFA54146AF3A1FDE6736EB8D4A
the State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics - No. T152805
ark:/67375/WNG-HF2PN4R0-J
the China Postdoctoral Science Foundation - No. 20090450065
the Science & Technology Commission of Shanghai Municipality - No. 08411961800
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ISSN:1001-604X
1614-7065
DOI:10.1002/cjoc.201180423