Study of the Anticarcinogenic Activity of Lactobacillus Bulgaricus in Diethylnitrosamine Induced Cancerogenesis in White Rats
The anticarcinogenic activity of living standardized culture of Lactobacillus delbruescii sub. bulgaricus 51-L (LB) was studied on male rats "Whistar", treated with diethylnitrosamine (DENA). The animals were divided into the following groups: I group-controls; II group-daily LB intake dos...
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Published in | Biotechnology, biotechnological equipment Vol. 11; no. 1-2; pp. 66 - 70 |
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Format | Journal Article |
Language | English |
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Taylor & Francis
01.01.1997
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Abstract | The anticarcinogenic activity of living standardized culture of Lactobacillus delbruescii sub. bulgaricus 51-L (LB) was studied on male rats "Whistar", treated with diethylnitrosamine (DENA). The animals were divided into the following groups: I group-controls; II group-daily LB intake dose of 2 ml/animal with the drinking water; III group-treated with DENA at a dose 130 mg/kg body weight injected intraperitoneally once per week for 8 consecutive weeks; IV group-treated simultaneously with LB and DENA. The animals were monitored during a period of 16 weeks. Their general condition, weight increase, mortality rate and manifestation of tumours were observed. After the observation period the animals were killed and the following parameters were studied: glutathione-S-transferase (G-S-T) activity in liver, macroscopic examination of the animals, histomorphological and histochemical investigation of the suspected ones for tumours and precancerogenic formations as well as liver, kidneys, spleen, lymph nodes, lungs and other (if necessary) target organs. An electronic-microscopic study of liver and morphological-immunologic investigation of spleens were carried out. A high mortality rate was established in DENA treated animals as well as cirrhotic changes of their liver which in the IV group showed a tendency for restriction. Specific G-S-T activity (calculated on the basis of soluble proteins) of liver showed a general tendency for increase in all groups at statistical significance in the group with the combined effect of DENA and LB. The induced tumour genesis mainly hepatic one affected 98% of the animals belonging to the III group and 83% of the animals of the IV group. A diagnostic of the induced benign and malignant tumours was performed. In the IV group statistically significant decrease (p < 0.01) of the number of tumours in a single animal was established versus that of the III group (1.6 versus 3.26), which is an evidence for the inhibiting effect of LB in relation to the DENA-induced cancerogenesis. Immuno-morphometric study on the spleens showed that in the IV group there was certain stimulation of the zones of B-lymphocytes at a preserved suppressive effect of DENA on the zones of T-lymphocytes. The possible mechanisms of the inhibiting effect of LB, which probably include activation of the detoxification mechanisms of G-S-T were also discussed. |
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AbstractList | The anticarcinogenic activity of living standardized culture of Lactobacillus delbruescii sub. bulgaricus 51-L (LB) was studied on male rats "Whistar", treated with diethylnitrosamine (DENA). The animals were divided into the following groups: I group-controls; II group-daily LB intake dose of 2 ml/animal with the drinking water; III group-treated with DENA at a dose 130 mg/kg body weight injected intraperitoneally once per week for 8 consecutive weeks; IV group-treated simultaneously with LB and DENA. The animals were monitored during a period of 16 weeks. Their general condition, weight increase, mortality rate and manifestation of tumours were observed. After the observation period the animals were killed and the following parameters were studied: glutathione-S-transferase (G-S-T) activity in liver, macroscopic examination of the animals, histomorphological and histochemical investigation of the suspected ones for tumours and precancerogenic formations as well as liver, kidneys, spleen, lymph nodes, lungs and other (if necessary) target organs. An electronic-microscopic study of liver and morphological-immunologic investigation of spleens were carried out. A high mortality rate was established in DENA treated animals as well as cirrhotic changes of their liver which in the IV group showed a tendency for restriction. Specific G-S-T activity (calculated on the basis of soluble proteins) of liver showed a general tendency for increase in all groups at statistical significance in the group with the combined effect of DENA and LB. The induced tumour genesis mainly hepatic one affected 98% of the animals belonging to the III group and 83% of the animals of the IV group. A diagnostic of the induced benign and malignant tumours was performed. In the IV group statistically significant decrease (p < 0.01) of the number of tumours in a single animal was established versus that of the III group (1.6 versus 3.26), which is an evidence for the inhibiting effect of LB in relation to the DENA-induced cancerogenesis. Immuno-morphometric study on the spleens showed that in the IV group there was certain stimulation of the zones of B-lymphocytes at a preserved suppressive effect of DENA on the zones of T-lymphocytes. The possible mechanisms of the inhibiting effect of LB, which probably include activation of the detoxification mechanisms of G-S-T were also discussed. |
Author | Vachkova-Petrova, R. Vassileva, L. Dinoeva, S. Donchev, N. Borov, B. |
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Cites_doi | 10.1093/jnci/50.3.815 10.1093/oxfordjournals.jbchem.a134464 10.1016/0027-5107(88)90195-9 10.1016/0192-0561(93)90028-W 10.4315/0362-028X-46.1.8 |
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References | Popova P. (CIT0014) 1993; 15 Bogdanov G. (CIT0008) 1973; 3 Reddy G.V. (CIT0016) 1983; 46 Barnes J.M. (CIT0005); 11 Turusov V. (CIT0018) 1976; 6 Donchev N. (CIT0003) 1993 Galkiiia O. (CIT0001) 1986; 1 Dinoeva S. (CIT0002) 1974; 3 Gichner T. (CIT0012) 1988; 202 Reddy G.V. (CIT0015) 1973; 50 Friend B.A. (CIT0010) 1982; 37 CIT0013 Davidkova G. (CIT0009) 1992; 14 Friend B. (CIT0011) 1984; 36 CIT0007 Shahani K.M. (CIT0017) 1969 Asaoka K. (CIT0004) 1983; 94 |
References_xml | – volume-title: Study on the biology and pathology of dimethylhydrazine tumor genesis of the large intestine under the influence of different nutritional regimens year: 1993 ident: CIT0003 contributor: fullname: Donchev N. – volume: 6 start-page: 1 year: 1976 ident: CIT0018 publication-title: Tumors of the liver. IARC Sci. Publ. contributor: fullname: Turusov V. – volume: 3 start-page: 85 year: 1974 ident: CIT0002 publication-title: Dynamics in the changes of immunolgical structures of spleen lymph follicles in pesticide intoxication. Gigiena i sanitaria contributor: fullname: Dinoeva S. – volume: 36 start-page: 125 year: 1984 ident: CIT0011 publication-title: J. Appl. Nutrition, Vol. contributor: fullname: Friend B. – ident: CIT0013 – volume: 50 start-page: 815 year: 1973 ident: CIT0015 publication-title: J. Natl. Cancer Inst. doi: 10.1093/jnci/50.3.815 contributor: fullname: Reddy G.V. – year: 1969 ident: CIT0017 publication-title: Isolation and study of anticarcinogenic agents from lactobacillus fermented food systems. Final report. Damon Runyan Memorial Fund for Cancer Research, Inc. contributor: fullname: Shahani K.M. – volume: 1 start-page: 101 year: 1986 ident: CIT0001 publication-title: Composition and biological action of the Lactobacillus bulgaricus-51 cell wall preparation, Biotechnology contributor: fullname: Galkiiia O. – volume: 37 start-page: 708 year: 1982 ident: CIT0010 publication-title: Michwissennschaft contributor: fullname: Friend B.A. – volume: 94 start-page: 1685 year: 1983 ident: CIT0004 publication-title: J. Biochemistry doi: 10.1093/oxfordjournals.jbchem.a134464 contributor: fullname: Asaoka K. – volume: 14 start-page: 1355 year: 1992 ident: CIT0009 publication-title: Endogenous production of tumor necrosis factor in normal mice orally treated with Deodan—a preparation from Lactobacillus bulgaricus “LB51”. Int.J.Immunopharm., Vol. contributor: fullname: Davidkova G. – volume: 202 start-page: 325 year: 1988 ident: CIT0012 publication-title: Mutation Res., Vol. doi: 10.1016/0027-5107(88)90195-9 contributor: fullname: Gichner T. – volume: 15 start-page: 25 year: 1993 ident: CIT0014 publication-title: Int. J. Immunopharm., Vol. doi: 10.1016/0192-0561(93)90028-W contributor: fullname: Popova P. – volume: 46 start-page: 8 year: 1983 ident: CIT0016 publication-title: J. Food Protection doi: 10.4315/0362-028X-46.1.8 contributor: fullname: Reddy G.V. – ident: CIT0007 – volume: 3 year: 1973 ident: CIT0008 publication-title: Cultivation of Lactobacillus bulgaricus to produce a ribonucleoproteid US Patent contributor: fullname: Bogdanov G. – volume: 11 start-page: 167 ident: CIT0005 publication-title: Some toxic properties of dimethylnitrosamine, Br.J.Ind.Med. contributor: fullname: Barnes J.M. |
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