Detection of Phytoplasma Associated with Periwinkle Virescence in Egypt

Little leaves, shortened internodes, virescence and witches' broom symptoms were observed affecting periwinkle plants growing in Kafr-el-sheikh governorate. These plants were examined for phytoplasma infections by electron microscopy. Phytoplasma appearing as spherical to ovoid structures of va...

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Published inPlant pathology journal Vol. 7; no. 1; pp. 92 - 97
Main Authors Omar, Ayman F., Emeran, Amero A., Abass, Jehan M.
Format Journal Article
LanguageEnglish
Published 15.12.2008
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Abstract Little leaves, shortened internodes, virescence and witches' broom symptoms were observed affecting periwinkle plants growing in Kafr-el-sheikh governorate. These plants were examined for phytoplasma infections by electron microscopy. Phytoplasma appearing as spherical to ovoid structures of variable sizes were observed in phloem sieve tubes in diseased plants samples but not in symptomless plants samples by TEM. PCR-amplifled phytoplasma of 16S rDNA using primers P1/P7 and PF2/PR2 was employed for the detection and identification of the phytoplasma associated with periwinkle plant. The Egyptian Phytoplasma Virescence (EPV) detected in diseased periwinkle (GenBank accession No. EF546439) was identified as members of aster yellow phytoplasma group (16SrI group) (Candidatus phytoplasma asteris). This is the first report on the occurrence of phytoplasma diseases in Egypt.
AbstractList Little leaves, shortened internodes, virescence and witches' broom symptoms were observed affecting periwinkle plants growing in Kafr-el-sheikh governorate. These plants were examined for phytoplasma infections by electron microscopy. Phytoplasma appearing as spherical to ovoid structures of variable sizes were observed in phloem sieve tubes in diseased plants samples but not in symptomless plants samples by TEM. PCR-amplifled phytoplasma of 16S rDNA using primers P1/P7 and PF2/PR2 was employed for the detection and identification of the phytoplasma associated with periwinkle plant. The Egyptian Phytoplasma Virescence (EPV) detected in diseased periwinkle (GenBank accession No. EF546439) was identified as members of aster yellow phytoplasma group (16SrI group) (Candidatus phytoplasma asteris). This is the first report on the occurrence of phytoplasma diseases in Egypt.
Author Abass, Jehan M.
Omar, Ayman F.
Emeran, Amero A.
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