CCoAOMT positively regulates the biosynthesis of secondary metabolites in Zanthoxylum armatum
IntroductionThe rust disease caused by the fungus Coleosporium zanthoxyli in Zanthoxylum armatum leads to significant leaf damage. However, the genetic regulatory mechanisms underlying disease resistance in this plant remain unclear.MethodsThrough transcriptome analysis and bioinformatics screening,...
Saved in:
| Published in | Frontiers in agronomy Vol. 7 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Frontiers Media S.A
28.07.2025
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 2673-3218 2673-3218 |
| DOI | 10.3389/fagro.2025.1604811 |
Cover
Loading…
| Abstract | IntroductionThe rust disease caused by the fungus Coleosporium zanthoxyli in Zanthoxylum armatum leads to significant leaf damage. However, the genetic regulatory mechanisms underlying disease resistance in this plant remain unclear.MethodsThrough transcriptome analysis and bioinformatics screening, the candidate gene CCoAOMT was identified as potentially involved in rust resistance. Genetic engineering techniques were employed to construct recombinant plasmids for both overexpression and RNAi mediated of CCoAOMT. Transient transformation and stable transformation methods successfully generated CCoAOMT-RNAi and CCoAOMT-overexpression transgenic plants and calli, respectively.ResultsqRT-PCR analysis revealed that the expression levels of CCoAOMT and six related genes were downregulated in RNAi-silenced plants but upregulated in overexpression lines. Following inoculation with fungal spore suspensions, CCoAOMT-RNAi plants exhibited significantly higher disease incidence and severity indices compared to wild-type controls, whereas overexpression plants showed the opposite trend with reduced susceptibility. Furthermore, total lignin and flavonoid contents were markedly decreased in RNAi lines and increased in overexpression lines relative to wild-type plants. Subcellular localization assays using GFP fusion constructs confirmed dual localization of CCoAOMT protein in both the cytoplasm and nucleus.ConclusionThese findings collectively demonstrate that CCoAOMT enhances rust resistance in Z. armatum by regulating lignin and flavonoid biosynthesis pathways. |
|---|---|
| AbstractList | IntroductionThe rust disease caused by the fungus Coleosporium zanthoxyli in Zanthoxylum armatum leads to significant leaf damage. However, the genetic regulatory mechanisms underlying disease resistance in this plant remain unclear.MethodsThrough transcriptome analysis and bioinformatics screening, the candidate gene CCoAOMT was identified as potentially involved in rust resistance. Genetic engineering techniques were employed to construct recombinant plasmids for both overexpression and RNAi mediated of CCoAOMT. Transient transformation and stable transformation methods successfully generated CCoAOMT-RNAi and CCoAOMT-overexpression transgenic plants and calli, respectively.ResultsqRT-PCR analysis revealed that the expression levels of CCoAOMT and six related genes were downregulated in RNAi-silenced plants but upregulated in overexpression lines. Following inoculation with fungal spore suspensions, CCoAOMT-RNAi plants exhibited significantly higher disease incidence and severity indices compared to wild-type controls, whereas overexpression plants showed the opposite trend with reduced susceptibility. Furthermore, total lignin and flavonoid contents were markedly decreased in RNAi lines and increased in overexpression lines relative to wild-type plants. Subcellular localization assays using GFP fusion constructs confirmed dual localization of CCoAOMT protein in both the cytoplasm and nucleus.ConclusionThese findings collectively demonstrate that CCoAOMT enhances rust resistance in Z. armatum by regulating lignin and flavonoid biosynthesis pathways. |
| Author | Li, Shujiang Xu, Xiu Han, Shan Hu, Yingxia Zhu, Tianhui Liu, Yinggao Yang, Chunlin Li, Shuying Lai, Yingming |
| Author_xml | – sequence: 1 givenname: Xiu surname: Xu fullname: Xu, Xiu – sequence: 2 givenname: Yingxia surname: Hu fullname: Hu, Yingxia – sequence: 3 givenname: Yingming surname: Lai fullname: Lai, Yingming – sequence: 4 givenname: Tianhui surname: Zhu fullname: Zhu, Tianhui – sequence: 5 givenname: Shujiang surname: Li fullname: Li, Shujiang – sequence: 6 givenname: Yinggao surname: Liu fullname: Liu, Yinggao – sequence: 7 givenname: Shuying surname: Li fullname: Li, Shuying – sequence: 8 givenname: Chunlin surname: Yang fullname: Yang, Chunlin – sequence: 9 givenname: Shan surname: Han fullname: Han, Shan |
| BookMark | eNpNkMtqwzAQRUVJoWmaH-hKP-BUD8eWl8H0EUjJJt0UihjJUqpgW0FySvP3VZpQupph5nK4nFs06n1vELqnZMa5qB4sbIOfMcLmM1qQXFB6hcasKHnGGRWjf_sNmsa4IyRFKWMkH6OPuvaL9esG7310g_sy7REHsz20MJiIh0-DlfPx2Kctuoi9xdFo3zcQjrgzAyjfulPS9fgdUsp_H9tDhyF0MBy6O3RtoY1mepkT9Pb0uKlfstX6eVkvVplmqUnGNDcCgEAJVW6FaOa6scxyUqSW6VMVlBa5KsXcVCUYWyjBVJM3ShteEEv5BC3P3MbDTu6D61I_6cHJ34MPWwlhcLo1cl5qpnJtNckTUpSgKVFlpUCZghLdJBY7s3TwMQZj_3iUyJNv-etbnnzLi2_-A54leHc |
| Cites_doi | 10.1111/tpj.15883 10.19303/j.issn.1008-0384.2023.05.013 10.1111/jipb.13213 10.1111/tpj.12140 10.3390/ijms24054879 10.1093/jxb/eraa204 10.1007/s00344-022-10872-2 10.1038/s41438-020-00376-z 10.11929/j.issn.2095-1914.2018.02.033 10.3390/ijms241813766 10.1016/S0021-9258(19)47389-4 10.1111/j.1469-8137.2005.01454.x 10.1111/j.1365-313X.2001.00956.x 10.13550/j.jxhg.20230866 10.16036/j.issn.1000-2650.202308300 10.13475/j.fzxb.2013.09.011 10.1016/j.scienta.2021.110282 10.3969/j.issn.1001-7461 10.1104/pp.16.00845 10.1016/j.ijbiomac 10.1016/j.plantsci.2021.111102 10.1186/s40168-022-01420-x 10.1590/S1415-47572013005000039 10.1016/j.cub.2012.04.064 10.1111/j.1469-8137.2011.03830.x 10.3969/j.issn.1005-9768 10.19415/j.cnki.1673-890x.2022.05.018 10.1128/EC.05109-11 10.1080/11263504.2019.1610107 10.3390/ijms23126760 10.1111/j.1469-8137.2008.02502.x 10.3969/j.issn.1003-5508 10.1007/s13258-019-00879-7 10.13482/j.issn1001-7011.2018.05.113 10.1038/s41438-021-00665-1 10.1007/s12298-023-01329-1 10.13271/j.mpb.017.001804 10.1094/PDIS-09-23-1880-RE 10.1016/j.molcel.2018.01.007 10.1016/j.phytochem.2019.03.004 10.1038/srep26458 10.3390/molecules24132452 10.11686/cyxb2020429 10.13348/j.cnki.sjlyyj.2020.0112.y 10.1007/s11103-011-9772-2 10.3969/j.issn.1004-1028 10.1016/j.plantsci.2023.111890 10.1080/13102818.2017.1304180 10.3390/ijms241914761 10.1146/annurev-arplant-010720-022215 10.4268/cjcmm20142312 10.1016/j.xplc.2024.101238 10.16779/j.cnki.1003-5508.2014.06.001 10.1093/pcp/pcp175 10.1016/j.foodres.2022.110948 10.3969/j.issn.1007-7731 10.3390/molecules28083599 10.1016/j.postharvbio.2015.03.008 |
| ContentType | Journal Article |
| DBID | AAYXX CITATION DOA |
| DOI | 10.3389/fagro.2025.1604811 |
| DatabaseName | CrossRef DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Agriculture |
| EISSN | 2673-3218 |
| ExternalDocumentID | oai_doaj_org_article_57c2b4cfc0464b87ac10b79babe610cd 10_3389_fagro_2025_1604811 |
| GroupedDBID | 9T4 AAFWJ AAHBH AAYXX ACXDI AFPKN ALMA_UNASSIGNED_HOLDINGS CITATION GROUPED_DOAJ M~E OK1 |
| ID | FETCH-LOGICAL-c2251-2c3e8aa0a7a94f88d5cdf2f306122e8a961164b785e97aef6b82bd4dbce360f13 |
| IEDL.DBID | DOA |
| ISSN | 2673-3218 |
| IngestDate | Wed Aug 27 01:30:41 EDT 2025 Thu Jul 31 00:04:16 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c2251-2c3e8aa0a7a94f88d5cdf2f306122e8a961164b785e97aef6b82bd4dbce360f13 |
| OpenAccessLink | https://doaj.org/article/57c2b4cfc0464b87ac10b79babe610cd |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_57c2b4cfc0464b87ac10b79babe610cd crossref_primary_10_3389_fagro_2025_1604811 |
| PublicationCentury | 2000 |
| PublicationDate | 2025-7-28 |
| PublicationDateYYYYMMDD | 2025-07-28 |
| PublicationDate_xml | – month: 07 year: 2025 text: 2025-7-28 day: 28 |
| PublicationDecade | 2020 |
| PublicationTitle | Frontiers in agronomy |
| PublicationYear | 2025 |
| Publisher | Frontiers Media S.A |
| Publisher_xml | – name: Frontiers Media S.A |
| References | Lin (B21) 2019; 17 Walker (B36) 2016; 172 Xu (B50) 2022; 314 Li (B19) 2020; 71 Luo (B23) 2022; 23 Yuan (B55) 2024 Guo (B14) 2023; 38 Lv (B24) 2018; 35 Gho (B13) 2020; 42 Wang (B41) 2005; 167 Ye (B53) 2020; 2020 Cao (B4) 2023; 29 Chen (B6) 2022; 16 Feng (B11) 2020; 2020 Cao (B5) 2025; 6 Ren (B31) 2024; 108 Mao (B26) 2014; 35 Zhu (B64) 2023; 2023 Dang (B8) 2011; 10 Wang (B38) 2013; 34 Wang (B40) 2020; 71 Zhuang (B65) 2023; 28 He (B16) 2023; 24 Yang (B52) 2023 Wu (B44) 2023; 29 Tang (B35) 2015; 30 Li (B18) 2023; 42 Ma (B25) 2021; 30 Zheng (B61) 2012; 33 Feng (B10) 2021; 8 Yang (B51) 2023 Wang (B37) 2021; 288 Yue (B57) 2010 Li (B20) 2013; 36 Purohit (B30) 2020; 154 Kumar (B17) 2016; 6 Sharma (B33) 2019; 24 You (B54) 2012 Zhou (B62) 2022; 111 (B28) 2020 Nakashima (B27) 2008; 179 Yuan (B56) 2024; 42 Alejandro (B2) 2012; 22 Parvathi (B29) 2001; 25 Zhao (B59) 2022; 64 Aktar-uz-zaman (B1) 2017; 31 Xu (B49) 2011; 192 Liu (B22) 2015; 105 Widiez (B43) 2011; 76 Xie (B48) 2014; 39 Cheng (B7) 2024; 2024 Wu (B45) 2022; 153 Wei (B42) 2023; 2023 Han (B15) 2023; 24 Xi (B46) 2018; 38 Bologna (B3) 2018; 69 Dare (B9) 2013; 74 Zhao (B60) 2021; 34 Gharibi (B12) 2019; 162 Zhou (B63) 2023; 24 Schmitt (B32) 1991; 266 Shi (B34) 2010; 51 Xia (B47) 2023; 242 Wang (B39) 2022; 10 Zhang (B58) 2010 |
| References_xml | – volume: 111 start-page: 1152 year: 2022 ident: B62 article-title: JrWRKY21 interacts with JrPTI5L to activate the expression of JrPR5L for resistance to Colletotrichum gloeosporioides in walnut publication-title: Plant J. doi: 10.1111/tpj.15883 – volume: 38 start-page: 616 year: 2023 ident: B14 article-title: Cloning and structural analysis of soybean caffeoyl coenzyme A-O-methyltransferase (CCoAOMT) gene publication-title: Fujian J. Agric. doi: 10.19303/j.issn.1008-0384.2023.05.013 – volume: 64 start-page: 476 year: 2022 ident: B59 article-title: RNA silencing: From discovery and elucidation to application and perspectives publication-title: J. Integr. Plant Biol. doi: 10.1111/jipb.13213 – volume-title: Mechanism of lignin synthesis regulation by VcMYB4a transcription factor in lingonberry and screening of interacting proteins year: 2023 ident: B52 – volume-title: Identification and control of major disease pathogens of pepper in Guizhou year: 2023 ident: B51 – volume: 74 start-page: 398 year: 2013 ident: B9 article-title: Phenotypic changes associated with RNA interference silencing of chalcone synthase in apple (Malus× domestica) publication-title: Plant J. doi: 10.1111/tpj.12140 – volume: 24 year: 2023 ident: B63 article-title: Changes in the Histology of Walnut (Juglans regia L.) Infected with Phomopsis capsici and Transcriptome and Metabolome Analysis publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms24054879 – volume: 71 start-page: 4715 year: 2020 ident: B40 article-title: Flavonoids are indispensable for complete male fertility in rice publication-title: J. Exp. Bot. doi: 10.1093/jxb/eraa204 – volume: 42 start-page: 4095 year: 2023 ident: B18 article-title: Increasing lignin accumulation in arabidopsis and poplar by overexpressing a CCoAOMT gene from the dove tree (Davidia involucrata baill.) publication-title: J. Plant Growth Regul. doi: 10.1007/s00344-022-10872-2 – volume: 2020 year: 2020 ident: B11 article-title: Genomic analysis reveals the genetic diversity, population structure, evolutionary history and relationships of Chinese pepper publication-title: Horticulture Res. doi: 10.1038/s41438-020-00376-z – volume: 38 start-page: 202 year: 2018 ident: B46 article-title: Analysis on the identification of a new disease of Dendrobium rosenbergii pepper sheath rust publication-title: J. Southwest Forestry Univ. (Natural Science) doi: 10.11929/j.issn.2095-1914.2018.02.033 – volume: 24 year: 2023 ident: B16 article-title: Cloning of PmMYB6 in Pinus massoniana and an analysis of its function publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms241813766 – volume: 266 start-page: 17416 year: 1991 ident: B32 article-title: Molecular cloning, induction and taxonomic distribution of caffeoyl-CoA 3-O-methyltransferase, an enzyme involved in disease resistance publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)47389-4 – volume: 167 start-page: 751 year: 2005 ident: B41 article-title: Assessment of penetrance and expressivity of RNAi-mediated silencing of the Arabidopsis phytoene desaturase gene publication-title: New Phytol. doi: 10.1111/j.1469-8137.2005.01454.x – volume: 25 start-page: 193 year: 2001 ident: B29 article-title: Substrate preferences of O-methyltransferases in alfalfa suggest new pathways for 3-O-methylation of monolignols publication-title: Plant J. doi: 10.1111/j.1365-313X.2001.00956.x – volume: 2024 start-page: 1 year: 2024 ident: B7 article-title: Preparation of liposomes of Vaccinium myrtillus leaf flavonoids and their effects on HepG2 cells publication-title: Fine Chem. Industry doi: 10.13550/j.jxhg.20230866 – volume: 42 start-page: 85 year: 2024 ident: B56 article-title: Isolation, identification and optimisation of spore-producing conditions of the pathogenic heavy parasitic bacteria of bamboo leaf pepper rust publication-title: J. Sichuan Agric. Univ. doi: 10.16036/j.issn.1000-2650.202308300 – volume-title: Pharmacopoeia of the People’s Republic of China - A Part year: 2020 ident: B28 – volume: 34 start-page: 12 year: 2013 ident: B38 article-title: Determination of lignin in cottonseed hulls by acetyl bromide method publication-title: Textile J. doi: 10.13475/j.fzxb.2013.09.011 – volume: 288 year: 2021 ident: B37 article-title: Transcriptomic analysis reveals a role of phenylpropanoid pathway in the enhancement of chilling tolerance by pre-storage cold acclimation in cucumber fruit publication-title: Scientia Hortic. doi: 10.1016/j.scienta.2021.110282 – volume: 30 start-page: 150 year: 2015 ident: B35 article-title: Research on the occurrence pattern and chemical control of pepper rust publication-title: J. Northwest Forestry Coll. doi: 10.3969/j.issn.1001-7461 – volume: 172 start-page: 78 year: 2016 ident: B36 article-title: The structure and catalytic mechanism of Sorghum bicolor caffeoyl-CoA O-methyltransferase publication-title: Plant Physiol. doi: 10.1104/pp.16.00845 – volume: 242 start-page: 124928 year: 2023 ident: B47 article-title: AcMADS32 positively regulates carotenoid biosynthesis in kiwifruit by activating AcBCH1/2 expression publication-title: Int. J. Biol. Macromolecules doi: 10.1016/j.ijbiomac – volume: 314 year: 2022 ident: B50 article-title: Molecular cloning and characterization of two distinct caffeoyl CoA O-methyltransferases (CCoAOMTs) from the liverwort Marchantia paleacea publication-title: Plant Sci. doi: 10.1016/j.plantsci.2021.111102 – volume: 10 start-page: 233 year: 2022 ident: B39 article-title: Multifaceted roles of flavonoids mediating plant-microbe interactions publication-title: Microbiome doi: 10.1186/s40168-022-01420-x – volume: 36 start-page: 540 year: 2013 ident: B20 article-title: Downregulation of caffeoyl-CoA O-methyltransferase (CCoAOMT) by RNA interference leads to reduced lignin production in maize straw publication-title: Genet. Mol. Biol. doi: 10.1590/S1415-47572013005000039 – volume: 22 start-page: 1207 year: 2012 ident: B2 article-title: AtABCG29 is a monolignol transporter involved in lignin biosynthesis publication-title: Curr. Biol. doi: 10.1016/j.cub.2012.04.064 – volume: 192 start-page: 611 year: 2011 ident: B49 article-title: Silencing of 4-coumarate: coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production publication-title: New Phytol. doi: 10.1111/j.1469-8137.2011.03830.x – volume: 2020 start-page: 70 year: 2020 ident: B53 article-title: Development status and prospect of Sichuan pepper industry publication-title: China Rural Sci. Technol. doi: 10.3969/j.issn.1005-9768 – volume: 16 start-page: 71 year: 2022 ident: B6 article-title: Optimisation of prevention and control technologies for pepper rust in Chongqing publication-title: South. Agric. doi: 10.19415/j.cnki.1673-890x.2022.05.018 – volume: 10 start-page: 1148 year: 2011 ident: B8 article-title: RNA interference in fungi: pathways, functions, and applications publication-title: Eukaryotic Cell doi: 10.1128/EC.05109-11 – start-page: 199 year: 2010 ident: B58 article-title: Taxonomic status and current status of compositional studies on green peppercorns publication-title: Northern Horticulture. – volume: 154 start-page: 288 year: 2020 ident: B30 article-title: Efficient plant regeneration through callus in Zanthoxylum armatum DC: an endangered medicinal plant of the Indian Himalayan region publication-title: Plant Biosystems-An Int. J. Dealing all Aspects Plant Biol. doi: 10.1080/11263504.2019.1610107 – volume-title: Establishment of a Molecular Detection System for CHINA Pepper Rust and Development Application of Biocontrol Agents year: 2024 ident: B55 – volume: 23 year: 2022 ident: B23 article-title: Molecular Mechanisms of Phenylpropane-Synthesis-Related Genes Regulating the Shoot Blight Resistance of Bambusa pervariabilis × Dendrocalamopsis grandis publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms23126760 – volume: 179 start-page: 738 year: 2008 ident: B27 article-title: Multi-site genetic modification of monolignol biosynthesis in alfalfa (Medicago sativa): effects on lignin composition in specific cell types publication-title: New Phytol. doi: 10.1111/j.1469-8137.2008.02502.x – volume: 33 start-page: 28 year: 2012 ident: B61 article-title: Research on the pathogenic bacteria of vine pepper rust publication-title: Sichuan Forestry Sci. Technol. doi: 10.3969/j.issn.1003-5508 – volume: 42 start-page: 67 year: 2020 ident: B13 article-title: Phenylalanine ammonia-lyase family is closely associated with response to phosphate deficiency in rice publication-title: Genes Genomics doi: 10.1007/s13258-019-00879-7 – volume-title: Taxonomy and molecular phylogeny of the Chinese sheath rust fungus year: 2012 ident: B54 – volume: 35 start-page: 317 year: 2018 ident: B24 article-title: Cloning, expression and bioinformatics analysis of BvM14-CCoAOMT gene in sugar beet publication-title: J. Natural Sci. Heilongjiang Univ. doi: 10.13482/j.issn1001-7011.2018.05.113 – volume: 8 year: 2021 ident: B10 article-title: Zanthoxylum-specific whole genome duplication and recent activity of transposable elements in the highly repetitive paleotetraploid Z. bungeanum genome publication-title: Horticulture Res. doi: 10.1038/s41438-021-00665-1 – volume: 29 start-page: 791 year: 2023 ident: B4 article-title: Two monolignoid biosynthetic genes 4-coumarate: coenzyme A ligase (4CL) and p-coumaric acid 3-hdroxylase (C3H) involved in lignin accumulation in pear fruits publication-title: Physiol. Mol. Biol. Plants doi: 10.1007/s12298-023-01329-1 – volume: 17 start-page: 1804 year: 2019 ident: B21 article-title: Cloning of tea tree CCoAOMT gene and structural analysis of promoter publication-title: Mol. Plant Breed. doi: 10.13271/j.mpb.017.001804 – volume: 108 year: 2024 ident: B31 article-title: Effect of Salicylic Acid Treatment on Disease Resistance to Coleosporium zanthoxyli in Chinese Pepper(Zanthoxylun armatun) publication-title: Plant Dis. doi: 10.1094/PDIS-09-23-1880-RE – volume: 69 start-page: 709 year: 2018 ident: B3 article-title: Nucleo-cytosolic shuttling of ARGONAUTE1 prompts a revised model of the plant microRNA pathway publication-title: Mol. Cell doi: 10.1016/j.molcel.2018.01.007 – volume: 162 start-page: 90 year: 2019 ident: B12 article-title: The effect of drought stress on polyphenolic compounds and expression of flavonoid biosynthesis related genes in Achillea pachycephala Rech. f publication-title: Phytochemistry doi: 10.1016/j.phytochem.2019.03.004 – volume: 6 year: 2016 ident: B17 article-title: RNAi down-regulation of cinnamate-4-hydroxylase increases artemisinin biosynthesis in Artemisia annua publication-title: Sci. Rep. doi: 10.1038/srep26458 – volume: 24 year: 2019 ident: B33 article-title: Response of phenylpropanoid pathway and the role of polyphenols in plants under abiotic stress publication-title: Molecules doi: 10.3390/molecules24132452 – volume: 30 start-page: 144 year: 2021 ident: B25 article-title: Identification, evolution and expression analysis of the CCoAOMT gene family in alfalfa publication-title: J. Grass Industry doi: 10.11686/cyxb2020429 – volume: 34 start-page: 32 year: 2021 ident: B60 article-title: Progress in tissue culture of pepper plants publication-title: World Forestry Res. doi: 10.13348/j.cnki.sjlyyj.2020.0112.y – volume: 76 start-page: 475 year: 2011 ident: B43 article-title: Functional characterization of two new members of the caffeoyl CoA O-methyltransferase-like gene family from Vanilla planifolia reveals a new class of plastid-localized O-methyltransferases publication-title: Plant Mol. Biol. doi: 10.1007/s11103-011-9772-2 – volume: 2023 start-page: 56 year: 2023 ident: B42 article-title: Hazards of pepper rust disease and comprehensive prevention and control measures publication-title: Sichuan Agric. Sci. Technol. doi: 10.3969/j.issn.1004-1028 – volume: 2023 year: 2023 ident: B64 article-title: The MPK6-LTF1L1 module regulates lignin biosynthesis in rice through a distinct mechanism from Populus LTF1 publication-title: Plant Sci. doi: 10.1016/j.plantsci.2023.111890 – volume: 31 start-page: 431 year: 2017 ident: B1 article-title: Genetic analysis of rust resistance genes in global wheat cultivars: an overview publication-title: Biotechnol. Biotechnol. Equip. doi: 10.1080/13102818.2017.1304180 – volume: 24 year: 2023 ident: B15 article-title: Integrated transcriptome and metabolome analysis reveals the molecular mechanism of rust resistance in resistant(Youkang) and susceptive(Tengjiao) zanthoxylun armatun cultivars publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms241914761 – volume: 71 start-page: 575 year: 2020 ident: B19 article-title: Exploiting broad-spectrum disease resistance in crops: from molecular dissection to breeding publication-title: Annu. Rev. Plant Biol. doi: 10.1146/annurev-arplant-010720-022215 – volume: 39 start-page: 4538 year: 2014 ident: B48 article-title: Overexpression and RNAi vectors built for key secondary metabolic pathway genes PAL, HMGR, PGT of Arnebia euchroma publication-title: China J. Chin. Materia Med. doi: 10.4268/cjcmm20142312 – volume: 6 year: 2025 ident: B5 article-title: Gap-free genome assemblies of two Pyrus bretschneideri cultivars and GWAS analyses identify a CCCH zinc finger protein as a key regulator of stone cell formation in pear fruit publication-title: Plant Commun. doi: 10.1016/j.xplc.2024.101238 – volume: 35 start-page: 1 year: 2014 ident: B26 article-title: Research on the incidence pattern and pharmaceutical control of vine pepper rust publication-title: Sichuan Forestry Sci. Technol. doi: 10.16779/j.cnki.1003-5508.2014.06.001 – volume: 51 start-page: 144 year: 2010 ident: B34 article-title: Towards a systems approach for lignin biosynthesis in Populus trichocarpa: transcript abundance and specificity of the monolignol biosynthetic genes publication-title: Plant Cell Physiol. doi: 10.1093/pcp/pcp175 – volume: 153 year: 2022 ident: B45 article-title: Integrative analysis of the metabolome and transcriptome provides insights into the mechanisms of flavonoid biosynthesis in blackberry publication-title: Food Res. Int. doi: 10.1016/j.foodres.2022.110948 – volume-title: Studies on the inherent resistance of pepper to Sphingomonas sp year: 2010 ident: B57 – volume: 29 start-page: 96 year: 2023 ident: B44 article-title: Major pests and diseases of pepper in Guizhou and their coping strategies publication-title: Anhui Agric. Bull. doi: 10.3969/j.issn.1007-7731 – volume: 28 year: 2023 ident: B65 article-title: The classification, molecular structure and biological biosynthesis of flavonoids, and their roles in biotic and abiotic stresses publication-title: Molecules doi: 10.3390/molecules28083599 – volume: 105 start-page: 45 year: 2015 ident: B22 article-title: Cloning and expression analysis of a CCoAOMT homolog in loquat fruit in response to low-temperature storage publication-title: Postharvest Biol. Technol. doi: 10.1016/j.postharvbio.2015.03.008 |
| SSID | ssj0002512204 |
| Score | 2.298498 |
| Snippet | IntroductionThe rust disease caused by the fungus Coleosporium zanthoxyli in Zanthoxylum armatum leads to significant leaf damage. However, the genetic... |
| SourceID | doaj crossref |
| SourceType | Open Website Index Database |
| SubjectTerms | CCoAOMT overexpression resistance genes RNAi Z. armatum |
| Title | CCoAOMT positively regulates the biosynthesis of secondary metabolites in Zanthoxylum armatum |
| URI | https://doaj.org/article/57c2b4cfc0464b87ac10b79babe610cd |
| Volume | 7 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LS8NAEF6kJz2IT6wv9uBNgsnmsZtjLS1FqF5aKIKE3c1uKdhEklTMxd_uTBKlnrx4CSEJYfkmmZlvdh6E3CjQii7n1tGRcp0AlKQT24g5vjSuKwOG5cuYbfEYTebBwyJcbI36wpywtj1wCxwQds1UoK3GPTgluNSeq3ispDJg-XWK2hds3haZQh2MVpu5QVslAywsvrNyWWCxHwsxoBIIz_tlibYa9jeWZXxA9juXkA7apRySHZMdkb3BsujaYphj8jIc5oOn6Yy2OVbv5rWmRTtF3pQUfDiqVnlZZ3BWrkqaW1oi0U1lUdO1qUDQWGpc0lVGnyW2C_ioQSlRDGdXm_UJmY9Hs-HE6QYjOBp-P89h2jdCSldyGQdWiDTUqWXWR3eFwZ048oAFKS5CE3NpbKQEU2mQKm1AMtbzT0kvyzNzRig30nLth6nVEVBJME6xDxQEnAwNvp0yfXL7DVLy1va_SIA3IKRJA2mCkCYdpH1yjzj-PIm9q5sLINGkk2jyl0TP_-MlF2QXF4ZRWCYuSa8qNuYK3IdKXTdfChynn6Mv0FXGKg |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=CCoAOMT+positively+regulates+the+biosynthesis+of+secondary+metabolites+in+Zanthoxylum+armatum&rft.jtitle=Frontiers+in+agronomy&rft.au=Xu%2C+Xiu&rft.au=Hu%2C+Yingxia&rft.au=Lai%2C+Yingming&rft.au=Zhu%2C+Tianhui&rft.date=2025-07-28&rft.issn=2673-3218&rft.eissn=2673-3218&rft.volume=7&rft_id=info:doi/10.3389%2Ffagro.2025.1604811&rft.externalDBID=n%2Fa&rft.externalDocID=10_3389_fagro_2025_1604811 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2673-3218&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2673-3218&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2673-3218&client=summon |