The effect of chronic exposure to fatty acids on gene expression in clonal insulin-producing cells: studies using high density oligonucleotide microarray
Fatty acids affect insulin secretion of pancreatic beta-cells. Investigating gene expression profiles may help to characterize the underlying mechanism. INS-1 cells were cultured with palmitate (0, 50, and 200 microM) for up to 44 d. Insulin secretion and expressions of 8740 genes were studied. We f...
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Published in | Endocrinology (Philadelphia) Vol. 142; no. 11; p. 4777 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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United States
01.11.2001
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Abstract | Fatty acids affect insulin secretion of pancreatic beta-cells. Investigating gene expression profiles may help to characterize the underlying mechanism. INS-1 cells were cultured with palmitate (0, 50, and 200 microM) for up to 44 d. Insulin secretion and expressions of 8740 genes were studied. We found that basal insulin secretion increased in cells exposed to palmitate. The response to glucose stimulation declined on d 44 in cells cultured at 200 microM palmitate. In response to 50 and 200 microM palmitate exposure, expression was changed in 11 and 99 genes on d 2 and 134 and in 159 genes on d 44, respectively. Genes involved in fatty acid oxidation were up-regulated, whereas those involved in glycolysis were down-regulated with 200 microM palmitate. A suppression of insulin receptor and insulin receptor substate-2 gene expression was found on d 44 in cells cultured at 200 microM palmitate. In conclusion, chronic exposure to low palmitate alters insulin secretion as well as gene expression. The number of genes that changed expression was palmitate dose and exposure time dependent. Randle's fatty acid-glucose cycle seems to be operative on the gene transcription level. A modification of expression of various genes may contribute to the functional changes. |
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AbstractList | Fatty acids affect insulin secretion of pancreatic beta-cells. Investigating gene expression profiles may help to characterize the underlying mechanism. INS-1 cells were cultured with palmitate (0, 50, and 200 microM) for up to 44 d. Insulin secretion and expressions of 8740 genes were studied. We found that basal insulin secretion increased in cells exposed to palmitate. The response to glucose stimulation declined on d 44 in cells cultured at 200 microM palmitate. In response to 50 and 200 microM palmitate exposure, expression was changed in 11 and 99 genes on d 2 and 134 and in 159 genes on d 44, respectively. Genes involved in fatty acid oxidation were up-regulated, whereas those involved in glycolysis were down-regulated with 200 microM palmitate. A suppression of insulin receptor and insulin receptor substate-2 gene expression was found on d 44 in cells cultured at 200 microM palmitate. In conclusion, chronic exposure to low palmitate alters insulin secretion as well as gene expression. The number of genes that changed expression was palmitate dose and exposure time dependent. Randle's fatty acid-glucose cycle seems to be operative on the gene transcription level. A modification of expression of various genes may contribute to the functional changes. |
Author | Ørntoft, T F Xiao, J Gregersen, S Hermansen, K Kruhøffer, M Pedersen, S B |
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CitedBy_id | crossref_primary_10_1016_j_nutres_2008_06_010 crossref_primary_10_1016_S0895_3988_10_60012_8 crossref_primary_10_1016_j_clnu_2006_05_008 crossref_primary_10_1016_j_bbagen_2007_07_001 crossref_primary_10_1016_S0958_1669_02_00349_X crossref_primary_10_1089_ars_2006_8_1 crossref_primary_10_1016_j_mce_2009_09_031 crossref_primary_10_1007_s12020_016_1051_1 crossref_primary_10_1016_j_niox_2004_01_005 crossref_primary_10_1530_JOE_12_0148 |
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Snippet | Fatty acids affect insulin secretion of pancreatic beta-cells. Investigating gene expression profiles may help to characterize the underlying mechanism. INS-1... |
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SubjectTerms | Cell Line Clone Cells - drug effects Clone Cells - metabolism Drug Synergism Fatty Acids - pharmacology Gene Expression - drug effects Glucose - pharmacology Insulin - biosynthesis Oligonucleotide Array Sequence Analysis Palmitates - pharmacology Reverse Transcriptase Polymerase Chain Reaction Time Factors |
Title | The effect of chronic exposure to fatty acids on gene expression in clonal insulin-producing cells: studies using high density oligonucleotide microarray |
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