Analysis of the Cellular Immune Responses to Vaccines

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1349; p. 247
• Main Authors: Svitek, Nicholas, Taracha, Evans L N, Saya, Rosemary, Awino, Elias, Nene, Vishvanath, Steinaa, Lucilla
• Format: Journal Article
• Language: English
• Published: United States 2016
• Subjects: Amino Acid Sequence - genetics; Animals; Cattle; Enzyme-Linked Immunospot Assay - methods; Epitopes, T-Lymphocyte - immunology; Flow Cytometry - methods; Immunity, Cellular - genetics; Immunity, Cellular - immunology; Livestock - virology; Peptides - immunology; T-Lymphocytes, Cytotoxic - immunology; Vaccines - immunology; Cytotoxicity assay; Flow cytometry; Peptide-MHC class I tetramers; CTL epitope; NetMHCpan; ELISpot

Flow cytometry, enzyme-linked immunospot (ELISpot) and cellular cytotoxicity assays are powerful tools for studying the cellular immune response towards intracellular pathogens and vaccines in livestock species. Lymphocytes from immunized animals can be purified using Ficoll-Paque density gradient c...