Hydrogen peroxide and Ascophyllum nodosum promote vigor in Cordia alliodora seeds at different maturation stages

• Published in: Australian Journal of Crop Science Vol. 19; no. 1; pp. 37 - 43
• Main Authors: Souza, Aline das Graças, Smiderle, Oscar José, Alves, Edna Ursulino
• Format: Journal Article
• Language: English
• Published: Lismore, N.S.W Southern Cross Publishers 2025
• Subjects: Acadians; Active oxygen; Ascophyllum nodosum; Cordia alliodora; Cordia goeldiana; Ethnic identity; Health aspects; Hydrogen peroxide; Therapeutic use
• ISSN: 1835-2693; 1835-2707
• DOI: 10.21475/ajcs.25.19.01.p156

Cordia alliodora is a species from the humid American tropics that produces valuable wood and is suitable for large-scale artificial regeneration. Its main areas of exploration and participation i production systems are in Central America and in the western and northwestern countries of the Brazilian Amazon. Cordia alliodora is a pioneer species that produces a large quantity of seed annually. Seedling emergence begins between 17 and 20 days after sowing, and May continue for another 20 days. Using solutions of hydrogen peroxide and phytohormones has been shown to be a important tool in the pre-germination stage of seeds of forest species, as it results in seedlings with greater vigor when the seeds are subjected to this chemical treatment. The objective was t determine the best concentrations of hydrogen peroxide and Ascophyllum nodosum for Cordia alliodora seeds at different maturation stages aiming at maximum seedling vigor. Experiment I: Th first experiment was conducted in a completely randomized design, in a 2x4 factorial scheme with two seed colors (green and brown) and four concentrations o Ascophyllum nodosum (0, 5.0, 10. and 15.0 ml L-1), with four replicates, each of which composed of 25 seeds. The variables evaluated in experiment I and II were obtained from the data collection performed by daily counts o emerged seedlings until the stabilization of emergence (28 DAS). The variables were: Emergence speed index (ESI), emergence percentage (E%) and mean time of emergence (MTE) Ascophyllum nodosum at concentration of 4.0 ml L-1 applied in green seeds of Cordia alliodora promotes 76.3% of seedling emergence. For brown seeds of Cordia alliodora, the use of hydrogen peroxide an Ascophyllum nodosum is not recommended. Experiment II: The second experiment was conducted in a completely randomized design, in a 2x4 factorial scheme with two seed colors (green an brown) and four concentrations of hydrogen peroxide (H2O2) (0, 20, 30 and 40 mM), with four replicates, each of which composed of 25 seeds. Treatments consisted of Cordia alliodora seeds (10 seeds per treatment) soaked in H2O2 solutions at concentrations of 0, 20, 30 and 40 mM for 30 minutes. The results obtained in this study show the existence of activation or inhibition of processe linked to root protrusion and seedling vigor, which is paramount to the success of the species under study. For green seeds of Cordia alliodora, the hydrogen peroxide concentration of 20 mM i indicated to obtain 90.8% of seedling emergence and increase uniformity in the stand of normal seedlings, promoting higher yield and vigor in Cordia alliodora seedlings. While the control showe 67,5% emergence of Cordia alliodora seedlings.