EXPRESSION FEATURES OF TOLL-LIKE RECEPTOR 2 AND TOLL-LIKE RECEPTOR 4 IN CHILDREN WITH ASTHMA

• Published in: Medit͡s︡inskai͡a︡ immunologii͡a Vol. 19; no. 4; pp. 431 - 440
• Main Authors: Gankovskaya, L. V., Namazova-Baranova, L. S., Khoreva, M. V., Bragvadze, B. G., Ogurtsova, A. D., Alekseeva, A. A., Gankovskii, V. А., Svitich, O. А.
• Format: Journal Article
• Language: English; Russian
• Published: St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists 01.08.2017
• Subjects: asthma; expression; flow cytometry; proinflammatory cytokines; tlr2; tlr4
• DOI: 10.15789/1563-0625-2017-4-431-440
• ISSN: 1563-0625

Currently actively  discussed  the  role  of innate immunity receptors, in particular TLRs  in the immunopathogenesis of bronchial asthma (BA).The aim of our work was to study the expression of ТLR2 and TLR4 on the nasal mucosal cells and peripheral blood leukocytes  of patients with BA of different  severity.The study included 40 children with asthma (3-12 years) and 10 healthy  children. Methods: real-time PCR, flow cytometry and multiplex immunofluorescence analysis evaluated the levels of pro and anti-inflammatory cytokines (IL-1β, IL-1ra, IL-6, IL-8, IL-10, TNFα) in nasal swabs.The result of the study hyperactivation of the factors of innate immunity at the level of the mucosal  of the nasal cavity in patients with asthma, manifested by increased gene expression  of TLR2, TLR4, and production of proinflammatory as well as anti-inflammatory cytokines. Correlation between cytokine levels and the severity of asthma. In the  peripheral blood  identified a significant  increase  in the  expression  of TLR2  and  TLR4  on circulating CD14+  monocytes in children with BA.Thus,  the  increase  of gene expression  of TLRs  mucosa of the  nasal cavity, increase  surface  expression  of TLR2  and TLR4  on circulating monocytes of patients with bronchial asthma compared to healthy  children. The revealed changes indicate the involvement of the system of TLRs in the immunopathogenesis of bronchial asthma. In the future, TLRs can be used as markers  to predict the course of ad and possible therapeutic targets.