Micro-arc driven porous ZrO2 coating for tailoring surface properties of titanium for dental implants application

Titanium (Ti) is an ideal material for dental implants due to its excellent properties. However, corrosion and mechanical wear lead to Ti ions and particles release, triggering inflammatory responses and bone resorption. To overcome these challenges, surface modification techniques are used, includi...

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Published inColloids and surfaces, B, Biointerfaces Vol. 245; p. 114237
Main Authors Andrade, Cátia Sufia, Borges, Maria Helena R., Silva, João Pedro, Malheiros, Samuel, Sacramento, Catharina, Ruiz, Karina G.S., da Cruz, Nilson C., Rangel, Elidiane C., Fortulan, Carlos, Figueiredo, Luciene, Nagay, Bruna E., Souza, Joāo Gabriel S., Barão, Valentim A.R.
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LanguageEnglish
Published Elsevier B.V 01.01.2025
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Abstract Titanium (Ti) is an ideal material for dental implants due to its excellent properties. However, corrosion and mechanical wear lead to Ti ions and particles release, triggering inflammatory responses and bone resorption. To overcome these challenges, surface modification techniques are used, including micro-arc oxidation (MAO). MAO creates adherent, porous coatings on Ti implants with diverse chemical compositions. In this context, zirconia element stands out in its wear and corrosion properties associated with low friction and chemical stability. Therefore, we investigated the impact of adding zirconium oxide (ZrO2) to Ti surfaces through MAO, aiming for improved electrochemical and mechanical properties. Additionally, the antimicrobial and modulatory potentials, cytocompatibility, and proteomic profile of surfaces were investigated. Ti discs were divided into four groups: machined – control (cpTi), treated by MAO with 0.04 M KOH – control (KOH), and two experimental groups incorporating ZrO2 at concentrations of 0.04 M and 0.08 M, composing the KOH@Zr4 and KOH@Zr8 groups. KOH@Zr8 showed higher surface porosity and roughness, even distribution of zirconia, formation of crystalline phases like ZrTiO4, and hydrophilicity. ZrO2 groups showed better mechanical performance including higher hardness values, lower wear area and mass loss, and higher friction coefficient under tribological conditions. The formation of a more compact oxide layer was observed, which favors the electrochemical stability of ZrO2 surfaces. Besides not inducing greater biofilm formation, ZrO2 surfaces reduced the load of pathogenic bacteria evidenced by the DNA-DNA checkerboard analysis. ZrO2 surfaces were cytocompatible with pre-osteoblastic cells. The saliva proteomic profile, evaluated by liquid chromatography coupled with tandem mass spectrometry, was slightly changed by zirconia, with more proteins adsorbed. KOH@Zr8 group notably absorbed proteins crucial for implant biological responses, like albumin and fibronectin. Incorporating ZrO2 improved the mechanical and electrochemical behavior of Ti surfaces, as well as modulated biofilm composition and provided suitable biological responses. [Display omitted] •ZrO2 coating was created on Ti surface using micro-arc oxidation.•ZrO2 enhances Ti implant surfaces with improved wear and corrosion resistance.•Reduced pathogenic bacteria was observed on ZrO2-modified surfaces.•ZrO2 surface adsorbs crucial human proteins.
AbstractList Titanium (Ti) is an ideal material for dental implants due to its excellent properties. However, corrosion and mechanical wear lead to Ti ions and particles release, triggering inflammatory responses and bone resorption. To overcome these challenges, surface modification techniques are used, including micro-arc oxidation (MAO). MAO creates adherent, porous coatings on Ti implants with diverse chemical compositions. In this context, zirconia element stands out in its wear and corrosion properties associated with low friction and chemical stability. Therefore, we investigated the impact of adding zirconium oxide (ZrO2) to Ti surfaces through MAO, aiming for improved electrochemical and mechanical properties. Additionally, the antimicrobial and modulatory potentials, cytocompatibility, and proteomic profile of surfaces were investigated. Ti discs were divided into four groups: machined – control (cpTi), treated by MAO with 0.04 M KOH – control (KOH), and two experimental groups incorporating ZrO2 at concentrations of 0.04 M and 0.08 M, composing the KOH@Zr4 and KOH@Zr8 groups. KOH@Zr8 showed higher surface porosity and roughness, even distribution of zirconia, formation of crystalline phases like ZrTiO4, and hydrophilicity. ZrO2 groups showed better mechanical performance including higher hardness values, lower wear area and mass loss, and higher friction coefficient under tribological conditions. The formation of a more compact oxide layer was observed, which favors the electrochemical stability of ZrO2 surfaces. Besides not inducing greater biofilm formation, ZrO2 surfaces reduced the load of pathogenic bacteria evidenced by the DNA-DNA checkerboard analysis. ZrO2 surfaces were cytocompatible with pre-osteoblastic cells. The saliva proteomic profile, evaluated by liquid chromatography coupled with tandem mass spectrometry, was slightly changed by zirconia, with more proteins adsorbed. KOH@Zr8 group notably absorbed proteins crucial for implant biological responses, like albumin and fibronectin. Incorporating ZrO2 improved the mechanical and electrochemical behavior of Ti surfaces, as well as modulated biofilm composition and provided suitable biological responses. [Display omitted] •ZrO2 coating was created on Ti surface using micro-arc oxidation.•ZrO2 enhances Ti implant surfaces with improved wear and corrosion resistance.•Reduced pathogenic bacteria was observed on ZrO2-modified surfaces.•ZrO2 surface adsorbs crucial human proteins.
Titanium (Ti) is an ideal material for dental implants due to its excellent properties. However, corrosion and mechanical wear lead to Ti ions and particles release, triggering inflammatory responses and bone resorption. To overcome these challenges, surface modification techniques are used, including micro-arc oxidation (MAO). MAO creates adherent, porous coatings on Ti implants with diverse chemical compositions. In this context, zirconia element stands out in its wear and corrosion properties associated with low friction and chemical stability. Therefore, we investigated the impact of adding zirconium oxide (ZrO2) to Ti surfaces through MAO, aiming for improved electrochemical and mechanical properties. Additionally, the antimicrobial and modulatory potentials, cytocompatibility, and proteomic profile of surfaces were investigated. Ti discs were divided into four groups: machined - control (cpTi), treated by MAO with 0.04 M KOH - control (KOH), and two experimental groups incorporating ZrO2 at concentrations of 0.04 M and 0.08 M, composing the KOH@Zr4 and KOH@Zr8 groups. KOH@Zr8 showed higher surface porosity and roughness, even distribution of zirconia, formation of crystalline phases like ZrTiO4, and hydrophilicity. ZrO2 groups showed better mechanical performance including higher hardness values, lower wear area and mass loss, and higher friction coefficient under tribological conditions. The formation of a more compact oxide layer was observed, which favors the electrochemical stability of ZrO2 surfaces. Besides not inducing greater biofilm formation, ZrO2 surfaces reduced the load of pathogenic bacteria evidenced by the DNA-DNA checkerboard analysis. ZrO2 surfaces were cytocompatible with pre-osteoblastic cells. The saliva proteomic profile, evaluated by liquid chromatography coupled with tandem mass spectrometry, was slightly changed by zirconia, with more proteins adsorbed. KOH@Zr8 group notably absorbed proteins crucial for implant biological responses, like albumin and fibronectin. Incorporating ZrO2 improved the mechanical and electrochemical behavior of Ti surfaces, as well as modulated biofilm composition and provided suitable biological responses.Titanium (Ti) is an ideal material for dental implants due to its excellent properties. However, corrosion and mechanical wear lead to Ti ions and particles release, triggering inflammatory responses and bone resorption. To overcome these challenges, surface modification techniques are used, including micro-arc oxidation (MAO). MAO creates adherent, porous coatings on Ti implants with diverse chemical compositions. In this context, zirconia element stands out in its wear and corrosion properties associated with low friction and chemical stability. Therefore, we investigated the impact of adding zirconium oxide (ZrO2) to Ti surfaces through MAO, aiming for improved electrochemical and mechanical properties. Additionally, the antimicrobial and modulatory potentials, cytocompatibility, and proteomic profile of surfaces were investigated. Ti discs were divided into four groups: machined - control (cpTi), treated by MAO with 0.04 M KOH - control (KOH), and two experimental groups incorporating ZrO2 at concentrations of 0.04 M and 0.08 M, composing the KOH@Zr4 and KOH@Zr8 groups. KOH@Zr8 showed higher surface porosity and roughness, even distribution of zirconia, formation of crystalline phases like ZrTiO4, and hydrophilicity. ZrO2 groups showed better mechanical performance including higher hardness values, lower wear area and mass loss, and higher friction coefficient under tribological conditions. The formation of a more compact oxide layer was observed, which favors the electrochemical stability of ZrO2 surfaces. Besides not inducing greater biofilm formation, ZrO2 surfaces reduced the load of pathogenic bacteria evidenced by the DNA-DNA checkerboard analysis. ZrO2 surfaces were cytocompatible with pre-osteoblastic cells. The saliva proteomic profile, evaluated by liquid chromatography coupled with tandem mass spectrometry, was slightly changed by zirconia, with more proteins adsorbed. KOH@Zr8 group notably absorbed proteins crucial for implant biological responses, like albumin and fibronectin. Incorporating ZrO2 improved the mechanical and electrochemical behavior of Ti surfaces, as well as modulated biofilm composition and provided suitable biological responses.
ArticleNumber 114237
Author Ruiz, Karina G.S.
Figueiredo, Luciene
Andrade, Cátia Sufia
Borges, Maria Helena R.
Rangel, Elidiane C.
da Cruz, Nilson C.
Fortulan, Carlos
Souza, Joāo Gabriel S.
Sacramento, Catharina
Nagay, Bruna E.
Malheiros, Samuel
Silva, João Pedro
Barão, Valentim A.R.
Author_xml – sequence: 1
  givenname: Cátia Sufia
  surname: Andrade
  fullname: Andrade, Cátia Sufia
  organization: Department of Prosthodontics and Periodontology, Piracicaba Dental School, Universidade Estadual de Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
– sequence: 2
  givenname: Maria Helena R.
  surname: Borges
  fullname: Borges, Maria Helena R.
  organization: Department of Prosthodontics and Periodontology, Piracicaba Dental School, Universidade Estadual de Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
– sequence: 3
  givenname: João Pedro
  surname: Silva
  fullname: Silva, João Pedro
  organization: Department of Prosthodontics and Periodontology, Piracicaba Dental School, Universidade Estadual de Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
– sequence: 4
  givenname: Samuel
  surname: Malheiros
  fullname: Malheiros, Samuel
  organization: Department of Prosthodontics and Periodontology, Piracicaba Dental School, Universidade Estadual de Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
– sequence: 5
  givenname: Catharina
  surname: Sacramento
  fullname: Sacramento, Catharina
  organization: Department of Prosthodontics and Periodontology, Piracicaba Dental School, Universidade Estadual de Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
– sequence: 6
  givenname: Karina G.S.
  surname: Ruiz
  fullname: Ruiz, Karina G.S.
  organization: Department of Prosthodontics and Periodontology, Piracicaba Dental School, Universidade Estadual de Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
– sequence: 7
  givenname: Nilson C.
  surname: da Cruz
  fullname: da Cruz, Nilson C.
  organization: Laboratory of Technological Plasmas, Engineering College, Univ Estadual Paulista (UNESP), Av Três de Março, 511, Sorocaba, São Paulo 18087-180, Brazil
– sequence: 8
  givenname: Elidiane C.
  surname: Rangel
  fullname: Rangel, Elidiane C.
  organization: Laboratory of Technological Plasmas, Engineering College, Univ Estadual Paulista (UNESP), Av Três de Março, 511, Sorocaba, São Paulo 18087-180, Brazil
– sequence: 9
  givenname: Carlos
  surname: Fortulan
  fullname: Fortulan, Carlos
  organization: Department of Mechanical Engineering, University of São Paulo (USP), Trabalhador São Carlense, 400, São Carlos, São Paulo 13566-590, Brazil
– sequence: 10
  givenname: Luciene
  surname: Figueiredo
  fullname: Figueiredo, Luciene
  organization: Dental Research Division, Guarulhos University, Guarulhos, São Paulo 07023-070, Brazil
– sequence: 11
  givenname: Bruna E.
  surname: Nagay
  fullname: Nagay, Bruna E.
  organization: Department of Prosthodontics and Periodontology, Piracicaba Dental School, Universidade Estadual de Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
– sequence: 12
  givenname: Joāo Gabriel S.
  surname: Souza
  fullname: Souza, Joāo Gabriel S.
  organization: Dental Research Division, Guarulhos University, Guarulhos, São Paulo 07023-070, Brazil
– sequence: 13
  givenname: Valentim A.R.
  surname: Barão
  fullname: Barão, Valentim A.R.
  email: vbarao@unicamp.br
  organization: Department of Prosthodontics and Periodontology, Piracicaba Dental School, Universidade Estadual de Campinas (UNICAMP), Av Limeira, 901, Piracicaba, São Paulo 13414-903, Brazil
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Snippet Titanium (Ti) is an ideal material for dental implants due to its excellent properties. However, corrosion and mechanical wear lead to Ti ions and particles...
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SubjectTerms Plasma electrolytic oxidation
Surface modification
Titanium implants
Zirconium oxide
Title Micro-arc driven porous ZrO2 coating for tailoring surface properties of titanium for dental implants application
URI https://dx.doi.org/10.1016/j.colsurfb.2024.114237
https://www.proquest.com/docview/3106732245
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