MST and TRIC Technology to Reliably Study PROTAC Binary and Ternary Binding in Drug Development

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2365; p. 115
• Main Authors: Bartoschik, Tanja, Zoephel, Andreas, Rumpel, Klaus, Ciulli, Alessio, Heffern, Charles
• Format: Journal Article
• Language: English
• Published: United States 2021
• Subjects: Drug Development; Intercellular Signaling Peptides and Proteins; Proteolysis; Technology; Temperature; Ubiquitin-Protein Ligases - metabolism; MicroScale Thermophoresis; Temperature-related intensity change; PROTAC; MST; TRIC

PROTACs have shown promise as a new class of therapy, with a unique mechanism of action orthogonal to traditional small molecules that are used to regulate protein activity. Their novel MOA utilizing the body's natural protein degradation machinery degrades a protein of interest rather than inh...