Protein Folding in the Absence of Chemical Denaturants

• Published in: The Journal of biological chemistry Vol. 274; no. 12; pp. 7732 - 7740
• Main Authors: Ronaldo Mohana-Borges, Jerson Lima Silva, Gonzalo de Prat-Gay
• Format: Journal Article
• Language: English
• Published: American Society for Biochemistry and Molecular Biology 01.03.1999

Small monomeric proteins are the best models for studying protein folding, but they are often too stable for denaturation using pressure as the sole perturbant. In the present work we subject [CI-2(1–40)·(41–64)], a noncovalent complex formed by the association of two complementary fragments of...