Evaluation of Glucose-6-Phosphate dehydrogenase in Saliva of Subjects with Normal Appearing Gingiva and Periodontitis using qRT-PCR: A Cross-sectional Study

To determine the salivary level of glucose-6-phosphate dehydrogenase (G6PD) in subjects with normal appearing gingiva and periodontitis, and to compare the salivary levels of G6PD in all the study groups. Unstimulated saliva (4 mL) was collected from 30 subjects; 15 periodontitis subjects and 15 con...

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Published inThe journal of contemporary dental practice Vol. 25; no. 11; pp. 1060 - 1068
Main Authors Srinath, Sahana, Khatoon, Hajira, Chavan, Ninad V, Yadava, Satish Thimmaiah, Shiragur, Savita, Purushothaman, Akalya, Setloor, Vaishnavi
Format Journal Article
LanguageEnglish
Published India 01.11.2024
Subjects
Adult
Biomarkers - analysis
Biomarkers - metabolism
Case-Control Studies
Cross-Sectional Studies
Female
Gingiva - enzymology
Glucosephosphate Dehydrogenase - analysis
Glucosephosphate Dehydrogenase - metabolism
Humans
Male
Middle Aged
Periodontitis - enzymology
Real-Time Polymerase Chain Reaction
Saliva - enzymology
Biomarkers
Oxidative stress
Glucose-6-phosphate dehydrogenase
Periodontitis
qRT-PCR Reactive oxygen species
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Abstract To determine the salivary level of glucose-6-phosphate dehydrogenase (G6PD) in subjects with normal appearing gingiva and periodontitis, and to compare the salivary levels of G6PD in all the study groups. Unstimulated saliva (4 mL) was collected from 30 subjects; 15 periodontitis subjects and 15 control subjects. Later, saliva samples were subjected to RNA extraction via TRIzol method. The RNA was converted to complementary DNA, and quantitative real time polymerase chain reaction was set-up. The expression of salivary G6PD in periodontitis subjects showed a significant increase when compared with control subjects, with a -value of 0.001 ( > 0.05). In conclusion, the overall results show promise in future aspects of G6PD as a potential diagnostic as well as a prognostic marker. However, large-scale, multi-institutional, and large sample sized studies should be conducted to further find out the importance of G6PD in the diagnosis of periodontitis and its prognostic value. The increase of G6PD levels in periodontitis subjects shows the increased oxidative stress, and this can be used for developing new noninvasive diagnostic procedures, developing novel treatment modalities, and also in assessing prognosis of treated cases of periodontitis. How to cite this article: Chavan NV, Srinath S, Yadava ST, Evaluation of Glucose-6-Phosphate dehydrogenase in Saliva of Subjects with Normal Appearing Gingiva and Periodontitis using qRT-PCR: A Cross-sectional Study. J Contemp Dent Pract 2024;25(11):1060-1068.
AbstractList To determine the salivary level of glucose-6-phosphate dehydrogenase (G6PD) in subjects with normal appearing gingiva and periodontitis, and to compare the salivary levels of G6PD in all the study groups. Unstimulated saliva (4 mL) was collected from 30 subjects; 15 periodontitis subjects and 15 control subjects. Later, saliva samples were subjected to RNA extraction via TRIzol method. The RNA was converted to complementary DNA, and quantitative real time polymerase chain reaction was set-up. The expression of salivary G6PD in periodontitis subjects showed a significant increase when compared with control subjects, with a -value of 0.001 ( > 0.05). In conclusion, the overall results show promise in future aspects of G6PD as a potential diagnostic as well as a prognostic marker. However, large-scale, multi-institutional, and large sample sized studies should be conducted to further find out the importance of G6PD in the diagnosis of periodontitis and its prognostic value. The increase of G6PD levels in periodontitis subjects shows the increased oxidative stress, and this can be used for developing new noninvasive diagnostic procedures, developing novel treatment modalities, and also in assessing prognosis of treated cases of periodontitis. How to cite this article: Chavan NV, Srinath S, Yadava ST, Evaluation of Glucose-6-Phosphate dehydrogenase in Saliva of Subjects with Normal Appearing Gingiva and Periodontitis using qRT-PCR: A Cross-sectional Study. J Contemp Dent Pract 2024;25(11):1060-1068.
To determine the salivary level of glucose-6-phosphate dehydrogenase (G6PD) in subjects with normal appearing gingiva and periodontitis, and to compare the salivary levels of G6PD in all the study groups.AIMSTo determine the salivary level of glucose-6-phosphate dehydrogenase (G6PD) in subjects with normal appearing gingiva and periodontitis, and to compare the salivary levels of G6PD in all the study groups.Unstimulated saliva (4 mL) was collected from 30 subjects; 15 periodontitis subjects and 15 control subjects. Later, saliva samples were subjected to RNA extraction via TRIzol method. The RNA was converted to complementary DNA, and quantitative real time polymerase chain reaction was set-up.MATERIALS AND METHODSUnstimulated saliva (4 mL) was collected from 30 subjects; 15 periodontitis subjects and 15 control subjects. Later, saliva samples were subjected to RNA extraction via TRIzol method. The RNA was converted to complementary DNA, and quantitative real time polymerase chain reaction was set-up.The expression of salivary G6PD in periodontitis subjects showed a significant increase when compared with control subjects, with a p-value of 0.001 (p > 0.05).RESULTSThe expression of salivary G6PD in periodontitis subjects showed a significant increase when compared with control subjects, with a p-value of 0.001 (p > 0.05).In conclusion, the overall results show promise in future aspects of G6PD as a potential diagnostic as well as a prognostic marker. However, large-scale, multi-institutional, and large sample sized studies should be conducted to further find out the importance of G6PD in the diagnosis of periodontitis and its prognostic value.CONCLUSIONIn conclusion, the overall results show promise in future aspects of G6PD as a potential diagnostic as well as a prognostic marker. However, large-scale, multi-institutional, and large sample sized studies should be conducted to further find out the importance of G6PD in the diagnosis of periodontitis and its prognostic value.The increase of G6PD levels in periodontitis subjects shows the increased oxidative stress, and this can be used for developing new noninvasive diagnostic procedures, developing novel treatment modalities, and also in assessing prognosis of treated cases of periodontitis. How to cite this article: Chavan NV, Srinath S, Yadava ST, et al. Evaluation of Glucose-6-Phosphate dehydrogenase in Saliva of Subjects with Normal Appearing Gingiva and Periodontitis using qRT-PCR: A Cross-sectional Study. J Contemp Dent Pract 2024;25(11):1060-1068.CLINICAL SIGNIFICANCEThe increase of G6PD levels in periodontitis subjects shows the increased oxidative stress, and this can be used for developing new noninvasive diagnostic procedures, developing novel treatment modalities, and also in assessing prognosis of treated cases of periodontitis. How to cite this article: Chavan NV, Srinath S, Yadava ST, et al. Evaluation of Glucose-6-Phosphate dehydrogenase in Saliva of Subjects with Normal Appearing Gingiva and Periodontitis using qRT-PCR: A Cross-sectional Study. J Contemp Dent Pract 2024;25(11):1060-1068.
Author Purushothaman, Akalya
Srinath, Sahana
Shiragur, Savita
Yadava, Satish Thimmaiah
Khatoon, Hajira
Setloor, Vaishnavi
Chavan, Ninad V
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Snippet To determine the salivary level of glucose-6-phosphate dehydrogenase (G6PD) in subjects with normal appearing gingiva and periodontitis, and to compare the...
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SubjectTerms Adult
Biomarkers - analysis
Biomarkers - metabolism
Case-Control Studies
Cross-Sectional Studies
Female
Gingiva - enzymology
Glucosephosphate Dehydrogenase - analysis
Glucosephosphate Dehydrogenase - metabolism
Humans
Male
Middle Aged
Periodontitis - enzymology
Real-Time Polymerase Chain Reaction
Saliva - enzymology
Title Evaluation of Glucose-6-Phosphate dehydrogenase in Saliva of Subjects with Normal Appearing Gingiva and Periodontitis using qRT-PCR: A Cross-sectional Study
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