Cloning and Nucleotide Sequences of livB and liv, the Structural Genes Encoding Binding Proteins of the High-Affinity Branched-Chain Amino Acid Transport in Salmonella typhimurium

• Published in: Journal of biochemistry (Tokyo) Vol. 107; no. 2; pp. 202 - 208
• Main Authors: Ohnishi, Kunibaru, Nakazima, Atsuko, Matsubara, Keiko, Kiritani, Kazuyoshi
• Format: Journal Article
• Language: English
• Published: Oxford University Press 01.02.1990

The liv gene cluster responsible for encoding the high-affinity .branched-chain amino acid transport proteins in Salmonella typhimurium was mapped in the 7.6-kilobase HindlU-SacI segment of plasmid pMN12 by utilizing the gene dosage effect. By subcloning and biochemical analysis, the livB and livC s...