Rapid identification of extracellular vesicles in basal tears using transmission electron microscopy

• Published in: Journal francais d'ophtalmologie Vol. 48; no. 4; p. 104446
• Main Authors: Catanese, S., Burlaud-Gaillard, J., Blasco, H., Blanchard, E., Pisella, P.-J., Khanna, R.K., Eymieux, S.
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.04.2025
• Subjects: Adult; Coloration négative; Extracellular vesicles; Extracellular Vesicles - ultrastructure; Female; Healthy Volunteers; Humans; Immunogold labelling; Immunohistochemistry; Larmes; Male; Marquage immunogold; Microscopie électronique à transmission; Microscopy, Electron, Transmission - methods; Middle Aged; Negative staining; Tears; Tears - chemistry; Tears - metabolism; Transmission electron microscopy; Vésicules extracellulaires; Young Adult; Negative staining; Marquage immunogold; Immunogold labelling; Larmes; Transmission electron microscopy; Extracellular vesicles; Coloration négative; Vésicules extracellulaires; Microscopie électronique à transmission; Tears

To describe basal human tear components in negative staining with real-time transmission electron microscopy and identify extracellular vesicles using immunogold labeling. Basal tears of 13 healthy human subjects were collected using microcapillary glass tubes, negatively stained, and observed by tr...