Activation of Sepharose with Epichiorohydrin and Subsequent Immobilization of Ligand for Affinity Adsorbent

• Published in: Journal of biochemistry (Tokyo) Vol. 85; no. 4; pp. 1091 - 1098
• Main Authors: MATSUMOTO, Isamu, MIZUNO, Yuko, SENO, Nobuko
• Format: Journal Article
• Language: English
• Published: Oxford University Press 01.04.1979

The optimal conditions for the activation of Sepharose by epichiorohydrin and subsequent immobilization of ligands were investigated. Under the optimal conditions for activation, namely, 30% Sepharose-5 % epichlorohydrin-0·4 M NaOH, 40°C, 2 h, the maximum amount of epoxy group was introduced into Se...