TIS21 /BTG2 inhibits doxorubicin-induced stress fiber-vimentin networks via Nox4-ROS-ABI2-DRF-linked signal cascade

• Published in: Cellular signalling Vol. 30; pp. 179 - 190
• Main Authors: Lim, In Kyoung, Choi, Jung-A, Kim, Eun Young, Kim, Bit Na, Jang, Soohyun, Ryu, Min Sook, Shim, Sang-Hee
• Format: Journal Article
• Language: English
• Published: England 01.01.2017
• Subjects: Actins - metabolism; Adaptor Proteins, Signal Transducing - metabolism; Cell Line, Tumor; Cellular Senescence - drug effects; Down-Regulation - drug effects; Doxorubicin - pharmacology; Humans; Immediate-Early Proteins - genetics; Immediate-Early Proteins - metabolism; Models, Biological; Multigene Family; NADPH Oxidase 4 - metabolism; Phenotype; Protein Stability - drug effects; Proteolysis - drug effects; Reactive Oxygen Species - metabolism; Signal Transduction - drug effects; Stress Fibers - drug effects; Stress Fibers - metabolism; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - metabolism; Vimentin - metabolism; Vimentin; NADPH oxidase 4 (Nox4); Actin stress fiber; Reactive oxygen species (ROS); Abl-interactor 2 (ABI2); Diaphanous-related formin (DRF)

Activities of TIS21 gene regulating cancer cell senescence were investigated in hepatoma cells by using low dose doxorubicin (Doxo, 100ng/mL). Treatment of Huh7 cells with Doxo increased linear actin nucleation e.g., transverse arcs and ventral stress fibers, as opposed to loss of filopodia. The lin...