The abasic site as a challenge to DNA polymerase
An abasic site in DNA creates a strong block to DNA polymerase and is a mutagenic base lesion. In this study, we present structural and dynamic properties of duplex oligodeoxynucleotides containing G, C and T opposite a model abasic site studied by one and two-dimensional nuclear magnetic resonance...
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Published in | Journal of molecular biology Vol. 213; no. 2; pp. 303 - 314 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
01.05.1990
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Abstract | An abasic site in DNA creates a strong block to DNA polymerase and is a mutagenic base lesion. In this study, we present structural and dynamic properties of duplex oligodeoxynucleotides containing G, C and T opposite a model abasic site studied by one and two-dimensional nuclear magnetic resonance spectroscopy. We have demonstrated that A opposite the abasic site was positioned within the helix as if paired with T, and that the A residue melted co-operatively with the surrounding helix. We report here that G opposite the abasic site is also observed to be predominantly intrahelical in a normal
anti conformation at low temperature. With increasing temperature, the mobility of the G residue increases rapidly and apparently is in a “melted state” well before denaturation of the helix. At low temperature, two species are found for T opposite the abasic site; one, intrahelical, one extrahelical. These species are in slow exchange with one another on a proton nuclear magnetic resonance time-scale. The two species then move into fast exchange with increasing temperature and the proportion of the extra-helical form increases. When C is positioned opposite the abasic site, both the C residue and the abasic sugar are extrahelical, the helix collapses, and the adjacent G·C base-pairs stack over one another. On the basis of these observations, we propose a model that explains why the abasic site acts to block DNA replication. Further, we suggest an explanation for the observed polymerase preference for base selection at abasic sites. |
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AbstractList | An abasic site in DNA creates a strong block to DNA polymerase and is a mutagenic base lesion. In this study, we present structural and dynamic properties of duplex oligodeoxynucleotides containing G, C and T opposite a model abasic site studied by one and two-dimensional nuclear magnetic resonance spectroscopy. We have demonstrated that A opposite the abasic site was positioned within the helix as if paired with T, and that the A residue melted co-operatively with the surrounding helix. We report here that G opposite the abasic site is also observed to be predominantly intrahelical in a normal
anti conformation at low temperature. With increasing temperature, the mobility of the G residue increases rapidly and apparently is in a “melted state” well before denaturation of the helix. At low temperature, two species are found for T opposite the abasic site; one, intrahelical, one extrahelical. These species are in slow exchange with one another on a proton nuclear magnetic resonance time-scale. The two species then move into fast exchange with increasing temperature and the proportion of the extra-helical form increases. When C is positioned opposite the abasic site, both the C residue and the abasic sugar are extrahelical, the helix collapses, and the adjacent G·C base-pairs stack over one another. On the basis of these observations, we propose a model that explains why the abasic site acts to block DNA replication. Further, we suggest an explanation for the observed polymerase preference for base selection at abasic sites. |
Author | Fazakerley, G.V. Guschlbauer, W. Kaplan, B.E. Cuniasse, Ph Sowers, L.C. |
Author_xml | – sequence: 1 givenname: Ph surname: Cuniasse fullname: Cuniasse, Ph – sequence: 2 givenname: G.V. surname: Fazakerley fullname: Fazakerley, G.V. – sequence: 3 givenname: W. surname: Guschlbauer fullname: Guschlbauer, W. – sequence: 4 givenname: B.E. surname: Kaplan fullname: Kaplan, B.E. – sequence: 5 givenname: L.C. surname: Sowers fullname: Sowers, L.C. |
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Cites_doi | 10.1093/nar/12.19.7435 10.1021/bi00404a019 10.1021/bi00288a026 10.1021/bi00431a009 10.1021/ja00295a052 10.1021/bi00294a038 10.1146/annurev.ge.20.120186.001221 10.1021/bi00293a016 10.1101/SQB.1987.052.01.053 10.1016/S0021-9258(18)48324-X 10.1002/bip.360261206 10.1021/bi00269a020 10.1002/bip.1981.360201208 10.1093/nar/15.19.8003 10.1080/15257778708073426 10.1016/0022-2836(79)90126-8 10.1021/bi00371a030 10.1080/07391102.1987.10506391 10.1016/0022-2836(83)90096-7 10.1021/bi00291a020 10.1021/bi00403a013 10.1021/bi00302a006 |
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References | Cuniasse, Sowers, Eritja, Kaplan, Goodman, Cognet, LeBret, Guschlbauer, Fazakerley (bib3) 1987; 15 Hare, Shapiro, Patel (bib12) 1987; 25 Kalnik, Chang, Grollman, Patel (bib14) 1988; 27 Flynn, Kintanar, Reid, Drobny (bib8) 1988; 27 Roy, Sklenar, Apella, Cohen (bib21) 1987; 26 Millican, Mock, Chauncey, Patel, Eaton, Grunning, Cutbush, Neidle, Mann (bib17) 1984; 12 Raap, Dreef, van der Marel, van Boom, Hilbers (bib19) 1987; 5 Randall, Eritja, Kaplan, Petruska, Goodman (bib20) 1987; 262 Kollman, Weiner, Dearing (bib15) 1981; 20 Loeb, Preston (bib16) 1986; 20 Davis, Bax (bib5) 1985; 107 Eritja, Walker, Randall, Goodman, Kaplan (bib6) 1987; 6 Shearman, Loeb (bib26) 1979; 128 Bodenhausen, Kogler, Ernst (bib1) 1984; 58 Boiteux, Laval (bib2) 1982; 21 Steitz, Beese, Freemont, Friedman, Sanderson (bib27) 1987; 52 Fréchet, Cheng, Kan, Ts'o (bib9) 1983; 22 Morden, Chu, Martin, Tinoco (bib18) 1983; 22 Hare, Wemmer, Chou, Drobny, Reid (bib11) 1983; 181 Hevroni, Livney (bib13) 1988; 85 Schaaper, Kunkel, Loeb (bib24) 1983; 80 Sagher, Strauss (bib22) 1983; 22 Schaaper, Glickman, Loeb (bib23) 1982; 42 Scheek, Boelens, Russo, van Boom, Kaptein (bib25) 1984; 23 Feigon, Leupin, Denny, Kearns (bib7) 1983; 22 Gait (bib10) 1984 Cuniasse, Sowers, Eritja, Kaplan, Goodman, Cognet, LeBret, Guschlbauer, Fazakerley (bib4) 1989; 28 Randall (10.1016/S0022-2836(05)80192-5_bib20) 1987; 262 Steitz (10.1016/S0022-2836(05)80192-5_bib27) 1987; 52 Morden (10.1016/S0022-2836(05)80192-5_bib18) 1983; 22 Cuniasse (10.1016/S0022-2836(05)80192-5_bib3) 1987; 15 Gait (10.1016/S0022-2836(05)80192-5_bib10) 1984 Fréchet (10.1016/S0022-2836(05)80192-5_bib9) 1983; 22 Kollman (10.1016/S0022-2836(05)80192-5_bib15) 1981; 20 Millican (10.1016/S0022-2836(05)80192-5_bib17) 1984; 12 Feigon (10.1016/S0022-2836(05)80192-5_bib7) 1983; 22 Loeb (10.1016/S0022-2836(05)80192-5_bib16) 1986; 20 Hare (10.1016/S0022-2836(05)80192-5_bib11) 1983; 181 Roy (10.1016/S0022-2836(05)80192-5_bib21) 1987; 26 Cuniasse (10.1016/S0022-2836(05)80192-5_bib4) 1989; 28 Eritja (10.1016/S0022-2836(05)80192-5_bib6) 1987; 6 Kalnik (10.1016/S0022-2836(05)80192-5_bib14) 1988; 27 Flynn (10.1016/S0022-2836(05)80192-5_bib8) 1988; 27 Shearman (10.1016/S0022-2836(05)80192-5_bib26) 1979; 128 Schaaper (10.1016/S0022-2836(05)80192-5_bib24) 1983; 80 Boiteux (10.1016/S0022-2836(05)80192-5_bib2) 1982; 21 Bodenhausen (10.1016/S0022-2836(05)80192-5_bib1) 1984; 58 Davis (10.1016/S0022-2836(05)80192-5_bib5) 1985; 107 Schaaper (10.1016/S0022-2836(05)80192-5_bib23) 1982; 42 Raap (10.1016/S0022-2836(05)80192-5_bib19) 1987; 5 Sagher (10.1016/S0022-2836(05)80192-5_bib22) 1983; 22 Hevroni (10.1016/S0022-2836(05)80192-5_bib13) 1988; 85 Scheek (10.1016/S0022-2836(05)80192-5_bib25) 1984; 23 Hare (10.1016/S0022-2836(05)80192-5_bib12) 1987; 25 |
References_xml | – volume: 6 start-page: 803 year: 1987 end-page: 814 ident: bib6 publication-title: Nucleos. Nucleot. contributor: fullname: Kaplan – volume: 15 start-page: 8003 year: 1987 end-page: 8022 ident: bib3 publication-title: Nucl. Acids Res. contributor: fullname: Fazakerley – volume: 27 start-page: 1191 year: 1988 end-page: 1197 ident: bib8 publication-title: Biochemistry contributor: fullname: Drobny – volume: 20 start-page: 2583 year: 1981 end-page: 2621 ident: bib15 publication-title: Biopolymers contributor: fullname: Dearing – volume: 22 start-page: 5943 year: 1983 end-page: 5951 ident: bib7 publication-title: Biochemistry contributor: fullname: Kearns – volume: 22 start-page: 5194 year: 1983 end-page: 5200 ident: bib9 publication-title: Biochemistry contributor: fullname: Ts'o – volume: 52 start-page: 465 year: 1987 end-page: 471 ident: bib27 publication-title: Cold Spring Harbor Symp. Quant. Biol. contributor: fullname: Sanderson – volume: 26 start-page: 2041 year: 1987 end-page: 2052 ident: bib21 publication-title: Biopolymers contributor: fullname: Cohen – volume: 22 start-page: 4518 year: 1983 end-page: 4526 ident: bib22 publication-title: Biochemistry contributor: fullname: Strauss – volume: 42 start-page: 3480 year: 1982 end-page: 3485 ident: bib23 publication-title: Cancer Res. contributor: fullname: Loeb – volume: 22 start-page: 5557 year: 1983 end-page: 5563 ident: bib18 publication-title: Biochemistry contributor: fullname: Tinoco – volume: 21 start-page: 6751 year: 1982 end-page: 6761 ident: bib2 publication-title: Biochemistry contributor: fullname: Laval – volume: 5 start-page: 219 year: 1987 end-page: 247 ident: bib19 publication-title: J. Biomol. Struct. Dynam. contributor: fullname: Hilbers – volume: 27 start-page: 924 year: 1988 end-page: 931 ident: bib14 publication-title: Biochemistry contributor: fullname: Patel – volume: 20 start-page: 201 year: 1986 end-page: 230 ident: bib16 publication-title: Annu. Rev. Genet. contributor: fullname: Preston – volume: 28 start-page: 2018 year: 1989 end-page: 2026 ident: bib4 publication-title: Biochemistry contributor: fullname: Fazakerley – volume: 107 start-page: 2820 year: 1985 end-page: 2821 ident: bib5 publication-title: J. Amer. Chem. Soc. contributor: fullname: Bax – volume: 128 start-page: 197 year: 1979 end-page: 218 ident: bib26 publication-title: J. Mol. Biol. contributor: fullname: Loeb – volume: 58 start-page: 370 year: 1984 end-page: 388 ident: bib1 publication-title: J. Magn. Reson. contributor: fullname: Ernst – volume: 23 start-page: 1371 year: 1984 end-page: 1376 ident: bib25 publication-title: Biochemistry contributor: fullname: Kaptein – volume: 181 start-page: 319 year: 1983 end-page: 336 ident: bib11 publication-title: J. Mol. Biol. contributor: fullname: Reid – volume: 12 start-page: 7435 year: 1984 end-page: 7453 ident: bib17 publication-title: Nucl. Acids Res. contributor: fullname: Mann – volume: 25 start-page: 7456 year: 1987 end-page: 7464 ident: bib12 publication-title: Biochemistry contributor: fullname: Patel – volume: 262 start-page: 6864 year: 1987 end-page: 6870 ident: bib20 publication-title: J. Biol. Chem. contributor: fullname: Goodman – volume: 80 start-page: 487 year: 1983 end-page: 491 ident: bib24 publication-title: Proc. Nat. Acad. Sci., U.S.A. contributor: fullname: Loeb – volume: 85 start-page: 5046 year: 1988 end-page: 5050 ident: bib13 publication-title: Proc. Nat. Acad. Sci., U.S.A. contributor: fullname: Livney – year: 1984 ident: bib10 article-title: Oligonucleotide Synthesis, A Practical Approach contributor: fullname: Gait – volume: 12 start-page: 7435 year: 1984 ident: 10.1016/S0022-2836(05)80192-5_bib17 publication-title: Nucl. Acids Res. doi: 10.1093/nar/12.19.7435 contributor: fullname: Millican – volume: 27 start-page: 1191 year: 1988 ident: 10.1016/S0022-2836(05)80192-5_bib8 publication-title: Biochemistry doi: 10.1021/bi00404a019 contributor: fullname: Flynn – volume: 85 start-page: 5046 year: 1988 ident: 10.1016/S0022-2836(05)80192-5_bib13 contributor: fullname: Hevroni – volume: 22 start-page: 4518 year: 1983 ident: 10.1016/S0022-2836(05)80192-5_bib22 publication-title: Biochemistry doi: 10.1021/bi00288a026 contributor: fullname: Sagher – volume: 28 start-page: 2018 year: 1989 ident: 10.1016/S0022-2836(05)80192-5_bib4 publication-title: Biochemistry doi: 10.1021/bi00431a009 contributor: fullname: Cuniasse – volume: 107 start-page: 2820 year: 1985 ident: 10.1016/S0022-2836(05)80192-5_bib5 publication-title: J. Amer. Chem. Soc. doi: 10.1021/ja00295a052 contributor: fullname: Davis – volume: 22 start-page: 5943 year: 1983 ident: 10.1016/S0022-2836(05)80192-5_bib7 publication-title: Biochemistry doi: 10.1021/bi00294a038 contributor: fullname: Feigon – volume: 20 start-page: 201 year: 1986 ident: 10.1016/S0022-2836(05)80192-5_bib16 publication-title: Annu. Rev. Genet. doi: 10.1146/annurev.ge.20.120186.001221 contributor: fullname: Loeb – volume: 22 start-page: 5557 year: 1983 ident: 10.1016/S0022-2836(05)80192-5_bib18 publication-title: Biochemistry doi: 10.1021/bi00293a016 contributor: fullname: Morden – volume: 80 start-page: 487 year: 1983 ident: 10.1016/S0022-2836(05)80192-5_bib24 contributor: fullname: Schaaper – volume: 42 start-page: 3480 year: 1982 ident: 10.1016/S0022-2836(05)80192-5_bib23 publication-title: Cancer Res. contributor: fullname: Schaaper – volume: 52 start-page: 465 year: 1987 ident: 10.1016/S0022-2836(05)80192-5_bib27 publication-title: Cold Spring Harbor Symp. Quant. Biol. doi: 10.1101/SQB.1987.052.01.053 contributor: fullname: Steitz – volume: 262 start-page: 6864 year: 1987 ident: 10.1016/S0022-2836(05)80192-5_bib20 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)48324-X contributor: fullname: Randall – volume: 26 start-page: 2041 year: 1987 ident: 10.1016/S0022-2836(05)80192-5_bib21 publication-title: Biopolymers doi: 10.1002/bip.360261206 contributor: fullname: Roy – volume: 21 start-page: 6751 year: 1982 ident: 10.1016/S0022-2836(05)80192-5_bib2 publication-title: Biochemistry doi: 10.1021/bi00269a020 contributor: fullname: Boiteux – volume: 20 start-page: 2583 year: 1981 ident: 10.1016/S0022-2836(05)80192-5_bib15 publication-title: Biopolymers doi: 10.1002/bip.1981.360201208 contributor: fullname: Kollman – volume: 58 start-page: 370 year: 1984 ident: 10.1016/S0022-2836(05)80192-5_bib1 publication-title: J. Magn. Reson. contributor: fullname: Bodenhausen – volume: 15 start-page: 8003 year: 1987 ident: 10.1016/S0022-2836(05)80192-5_bib3 publication-title: Nucl. Acids Res. doi: 10.1093/nar/15.19.8003 contributor: fullname: Cuniasse – volume: 6 start-page: 803 year: 1987 ident: 10.1016/S0022-2836(05)80192-5_bib6 publication-title: Nucleos. Nucleot. doi: 10.1080/15257778708073426 contributor: fullname: Eritja – volume: 128 start-page: 197 year: 1979 ident: 10.1016/S0022-2836(05)80192-5_bib26 publication-title: J. Mol. Biol. doi: 10.1016/0022-2836(79)90126-8 contributor: fullname: Shearman – volume: 25 start-page: 7456 year: 1987 ident: 10.1016/S0022-2836(05)80192-5_bib12 publication-title: Biochemistry doi: 10.1021/bi00371a030 contributor: fullname: Hare – volume: 5 start-page: 219 year: 1987 ident: 10.1016/S0022-2836(05)80192-5_bib19 publication-title: J. Biomol. Struct. Dynam. doi: 10.1080/07391102.1987.10506391 contributor: fullname: Raap – year: 1984 ident: 10.1016/S0022-2836(05)80192-5_bib10 contributor: fullname: Gait – volume: 181 start-page: 319 year: 1983 ident: 10.1016/S0022-2836(05)80192-5_bib11 publication-title: J. Mol. Biol. doi: 10.1016/0022-2836(83)90096-7 contributor: fullname: Hare – volume: 22 start-page: 5194 year: 1983 ident: 10.1016/S0022-2836(05)80192-5_bib9 publication-title: Biochemistry doi: 10.1021/bi00291a020 contributor: fullname: Fréchet – volume: 27 start-page: 924 year: 1988 ident: 10.1016/S0022-2836(05)80192-5_bib14 publication-title: Biochemistry doi: 10.1021/bi00403a013 contributor: fullname: Kalnik – volume: 23 start-page: 1371 year: 1984 ident: 10.1016/S0022-2836(05)80192-5_bib25 publication-title: Biochemistry doi: 10.1021/bi00302a006 contributor: fullname: Scheek |
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Snippet | An abasic site in DNA creates a strong block to DNA polymerase and is a mutagenic base lesion. In this study, we present structural and dynamic properties of... |
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Title | The abasic site as a challenge to DNA polymerase |
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