Similarities of physico-chemical and antigenic properties of neurocupreins and extremely acidic copper proteins from chromaffin granules

Extremely acidic copper-containing proteins, neurocupreins, were isolated from brains of various mammals (bovine, rabbit, pig and sheep). Neurocupreins from all these sources were found to have similar physico-chemical and antigenic properties. Using the immunological approach, it was shown that neu...

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Published inBiochimica et biophysica acta. General subjects Vol. 924; no. 3; pp. 548 - 555
Main Authors Mikaelyan, Mariam V., Grigoryan, Nvard A., Nalbandyan, Robert M.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 22.06.1987
Subjects
Acidic protein
Antibody
Chromaffin granule
Copper protein
Neurocuprein
Copper protein
Chromaffin granule
Neurocuprein
Antibody
Acidic protein
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Abstract Extremely acidic copper-containing proteins, neurocupreins, were isolated from brains of various mammals (bovine, rabbit, pig and sheep). Neurocupreins from all these sources were found to have similar physico-chemical and antigenic properties. Using the immunological approach, it was shown that neurocuprein is located only in brain cytosol and synaptosomal fractions. Extremely acidic copper-containing proteins were also isolated from soluble and membranous fractions of chromaffin granules from bovine adrenal medulla. The soluble form of the protein from the granules has practically the same physico-chemical and antigenic properties as neurocupreins. The copper protein isolated from membranes of granules has slightly higher molecular weight and somewhat different amino acid composition, although their EPR spectra are identical. However, both copper proteins from chromaffin granules are immunoprecipitated with antibodies to neurocuprein. It is suggested the the membranous form differs from the soluble one in possessing a peptide which prolongs the protein chain without changes in its antigenic properties.
AbstractList Extremely acidic copper-containing proteins, neurocupreins, were isolated from brains of various mammals (bovine, rabbit, pig and sheep). Neurocupreins from all these sources were found to have similar physico-chemical and antigenic properties. Using the immunological approach, it was shown that neurocuprein is located only in brain cytosol and synaptosomal fractions. Extremely acidic copper-containing proteins were also isolated from soluble and membranous fractions of chromaffin granules from bovine adrenal medulla. The soluble form of the protein from the granules has practically the same physico-chemical and antigenic properties as neurocupreins. The copper protein isolated from membranes of granules has slightly higher molecular weight and somewhat different amino acid composition, although their EPR spectra are identical. However, both copper proteins from chromaffin granules are immunoprecipitated with antibodies to neurocuprein. It is suggested the the membranous form differs from the soluble one in possessing a peptide which prolongs the protein chain without changes in its antigenic properties.
Author Grigoryan, Nvard A.
Mikaelyan, Mariam V.
Nalbandyan, Robert M.
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Keywords Copper protein
Chromaffin granule
Neurocuprein
Antibody
Acidic protein
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Snippet Extremely acidic copper-containing proteins, neurocupreins, were isolated from brains of various mammals (bovine, rabbit, pig and sheep). Neurocupreins from...
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StartPage 548
SubjectTerms Acidic protein
Antibody
Chromaffin granule
Copper protein
Neurocuprein
Title Similarities of physico-chemical and antigenic properties of neurocupreins and extremely acidic copper proteins from chromaffin granules
URI https://dx.doi.org/10.1016/0304-4165(87)90171-1
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