Tailored biomimetic hydrogel based on a photopolymerised DMP1/MCF/gelatin hybrid system for calvarial bone regeneration
Searching for effective osteoinduction factors with higher specificity and biosafety for the preparation of biomimetic materials, which mimic the natural bone extracellular matrix (ECM), seems to be an optimum strategy for achieving ideal bone regeneration. Dentin matrix protein 1 (DMP1) seems to be...
Saved in:
Published in | Journal of materials chemistry. B, Materials for biology and medicine Vol. 6; no. 3; pp. 414 - 427 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Royal Society of Chemistry
21.01.2018
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Searching for effective osteoinduction factors with higher specificity and biosafety for the preparation of biomimetic materials, which mimic the natural bone extracellular matrix (ECM), seems to be an optimum strategy for achieving ideal bone regeneration. Dentin matrix protein 1 (DMP1) seems to be a highly promising candidate due to its pleiotropic bio-regulation roles in several bone formation processes including osteoinduction, osteogenesis and biomineralization. In this study, we first generated a novel meso/macro-structured photopolymerised hybrid hydrogel system, in which DMP1 was loaded in mesocellular silica foam (MCF) and then evenly embedded in the photopolymerised gelatin hydrogel. This DMP1-loaded hybrid system (DMP1/MCF/gel) exhibited a hierarchical porous structure according to the surface area and pore size distribution analysis, TEM and SEM. Protein loading quantification and release kinetics experiments demonstrated that DMP1 is well protected and stably released for a longer duration in the DMP1/MCF/gel system. The expression levels of bone markers in the BMSCs under the co-treatment of both Si ion and DMP1 suggested there was a synergistic effect between Si ion and DMP1, both of which could be released from DMP1/MCF/gel system. Further
in vitro
osteoinductive experiments of bone marrow stem cells (BMSCs) showed that the DMP1/MCF/gel system enhanced the activity of alkaline phosphatase (ALP), promoted the formation of mineralized bone nodules, and up-regulated the expression levels of several osteogenic markers (including
Runx2
,
Osx
,
Bsp
,
Ocn
,
Opn
, and
Dmp1
). In addition, both µ-CT and histological studies suggested that the
in vivo
repair of rat calvarial defects using the DMP1/MCF/gel system led to accelerated bone regeneration with better mineralization and higher quality. In summary, this work generated a tailored biomimetic hydrogel based on a photopolymerised DMP1/MCF/gel hybrid system, which mimicked the non-mineralized bone ECM and resulted in improved bone regeneration.
Searching for effective osteoinduction factors with higher specificity and biosafety for the preparation of biomimetic materials, which mimic the natural bone extracellular matrix (ECM), seems to be an optimum strategy for achieving ideal bone regeneration. |
---|---|
AbstractList | Searching for effective osteoinduction factors with higher specificity and biosafety for the preparation of biomimetic materials, which mimic the natural bone extracellular matrix (ECM), seems to be an optimum strategy for achieving ideal bone regeneration. Dentin matrix protein 1 (DMP1) seems to be a highly promising candidate due to its pleiotropic bio-regulation roles in several bone formation processes including osteoinduction, osteogenesis and biomineralization. In this study, we first generated a novel meso/macro-structured photopolymerised hybrid hydrogel system, in which DMP1 was loaded in mesocellular silica foam (MCF) and then evenly embedded in the photopolymerised gelatin hydrogel. This DMP1-loaded hybrid system (DMP1/MCF/gel) exhibited a hierarchical porous structure according to the surface area and pore size distribution analysis, TEM and SEM. Protein loading quantification and release kinetics experiments demonstrated that DMP1 is well protected and stably released for a longer duration in the DMP1/MCF/gel system. The expression levels of bone markers in the BMSCs under the co-treatment of both Si ion and DMP1 suggested there was a synergistic effect between Si ion and DMP1, both of which could be released from DMP1/MCF/gel system. Further in vitro osteoinductive experiments of bone marrow stem cells (BMSCs) showed that the DMP1/MCF/gel system enhanced the activity of alkaline phosphatase (ALP), promoted the formation of mineralized bone nodules, and up-regulated the expression levels of several osteogenic markers (including Runx2, Osx, Bsp, Ocn, Opn, and Dmp1). In addition, both µ-CT and histological studies suggested that the in vivo repair of rat calvarial defects using the DMP1/MCF/gel system led to accelerated bone regeneration with better mineralization and higher quality. In summary, this work generated a tailored biomimetic hydrogel based on a photopolymerised DMP1/MCF/gel hybrid system, which mimicked the non-mineralized bone ECM and resulted in improved bone regeneration. Searching for effective osteoinduction factors with higher specificity and biosafety for the preparation of biomimetic materials, which mimic the natural bone extracellular matrix (ECM), seems to be an optimum strategy for achieving ideal bone regeneration. Dentin matrix protein 1 (DMP1) seems to be a highly promising candidate due to its pleiotropic bio-regulation roles in several bone formation processes including osteoinduction, osteogenesis and biomineralization. In this study, we first generated a novel meso/macro-structured photopolymerised hybrid hydrogel system, in which DMP1 was loaded in mesocellular silica foam (MCF) and then evenly embedded in the photopolymerised gelatin hydrogel. This DMP1-loaded hybrid system (DMP1/MCF/gel) exhibited a hierarchical porous structure according to the surface area and pore size distribution analysis, TEM and SEM. Protein loading quantification and release kinetics experiments demonstrated that DMP1 is well protected and stably released for a longer duration in the DMP1/MCF/gel system. The expression levels of bone markers in the BMSCs under the co-treatment of both Si ion and DMP1 suggested there was a synergistic effect between Si ion and DMP1, both of which could be released from DMP1/MCF/gel system. Further in vitro osteoinductive experiments of bone marrow stem cells (BMSCs) showed that the DMP1/MCF/gel system enhanced the activity of alkaline phosphatase (ALP), promoted the formation of mineralized bone nodules, and up-regulated the expression levels of several osteogenic markers (including Runx2 , Osx , Bsp , Ocn , Opn , and Dmp1 ). In addition, both µ-CT and histological studies suggested that the in vivo repair of rat calvarial defects using the DMP1/MCF/gel system led to accelerated bone regeneration with better mineralization and higher quality. In summary, this work generated a tailored biomimetic hydrogel based on a photopolymerised DMP1/MCF/gel hybrid system, which mimicked the non-mineralized bone ECM and resulted in improved bone regeneration. Searching for effective osteoinduction factors with higher specificity and biosafety for the preparation of biomimetic materials, which mimic the natural bone extracellular matrix (ECM), seems to be an optimum strategy for achieving ideal bone regeneration. Searching for effective osteoinduction factors with higher specificity and biosafety for the preparation of biomimetic materials, which mimic the natural bone extracellular matrix (ECM), seems to be an optimum strategy for achieving ideal bone regeneration. Dentin matrix protein 1 (DMP1) seems to be a highly promising candidate due to its pleiotropic bio-regulation roles in several bone formation processes including osteoinduction, osteogenesis and biomineralization. In this study, we first generated a novel meso/macro-structured photopolymerised hybrid hydrogel system, in which DMP1 was loaded in mesocellular silica foam (MCF) and then evenly embedded in the photopolymerised gelatin hydrogel. This DMP1-loaded hybrid system (DMP1/MCF/gel) exhibited a hierarchical porous structure according to the surface area and pore size distribution analysis, TEM and SEM. Protein loading quantification and release kinetics experiments demonstrated that DMP1 is well protected and stably released for a longer duration in the DMP1/MCF/gel system. The expression levels of bone markers in the BMSCs under the co-treatment of both Si ion and DMP1 suggested there was a synergistic effect between Si ion and DMP1, both of which could be released from DMP1/MCF/gel system. Further in vitro osteoinductive experiments of bone marrow stem cells (BMSCs) showed that the DMP1/MCF/gel system enhanced the activity of alkaline phosphatase (ALP), promoted the formation of mineralized bone nodules, and up-regulated the expression levels of several osteogenic markers (including Runx2 , Osx , Bsp , Ocn , Opn , and Dmp1 ). In addition, both µ-CT and histological studies suggested that the in vivo repair of rat calvarial defects using the DMP1/MCF/gel system led to accelerated bone regeneration with better mineralization and higher quality. In summary, this work generated a tailored biomimetic hydrogel based on a photopolymerised DMP1/MCF/gel hybrid system, which mimicked the non-mineralized bone ECM and resulted in improved bone regeneration. |
Author | Cao, Lingyan Du, Jiahui Jiao, Delong Gan, Qi Duan, Shengzhong Jiang, Xinquan Wang, Qian Wu, Jiannan Lin, Shuxian |
AuthorAffiliation | Ninth People's Hospital Stomatological Hospital of Chongqing Medical University Ninth People's Hospital affiliated to Shanghai Jiao Tong University Shanghai Research Institute of Stomatology Department of Oral and Maxillofacial Surgery Shanghai Key Laboratory of Stomatology East China University of Science and Technology Laboratory of Oral Microbiota and Systemic Diseases Department of Prosthodontics The State Key Laboratory of Bioreactor Engineering Oral Bioengineering Lab School of Medicine School of Stomatology |
AuthorAffiliation_xml | – name: School of Medicine – name: Ninth People's Hospital affiliated to Shanghai Jiao Tong University – name: Oral Bioengineering Lab – name: Shanghai Key Laboratory of Stomatology – name: Ninth People's Hospital – name: The State Key Laboratory of Bioreactor Engineering – name: Stomatological Hospital of Chongqing Medical University – name: Department of Prosthodontics – name: School of Stomatology – name: East China University of Science and Technology – name: Shanghai Research Institute of Stomatology – name: Laboratory of Oral Microbiota and Systemic Diseases – name: Department of Oral and Maxillofacial Surgery |
Author_xml | – sequence: 1 givenname: Shuxian surname: Lin fullname: Lin, Shuxian – sequence: 2 givenname: Lingyan surname: Cao fullname: Cao, Lingyan – sequence: 3 givenname: Qian surname: Wang fullname: Wang, Qian – sequence: 4 givenname: Jiahui surname: Du fullname: Du, Jiahui – sequence: 5 givenname: Delong surname: Jiao fullname: Jiao, Delong – sequence: 6 givenname: Shengzhong surname: Duan fullname: Duan, Shengzhong – sequence: 7 givenname: Jiannan surname: Wu fullname: Wu, Jiannan – sequence: 8 givenname: Qi surname: Gan fullname: Gan, Qi – sequence: 9 givenname: Xinquan surname: Jiang fullname: Jiang, Xinquan |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32254521$$D View this record in MEDLINE/PubMed |
BookMark | eNp9kctPGzEQxq2KqgGaS--tjLghhYzt9T6OJTyKRFQOQept5cdsMNpdb-1Nq_z3GALprXPxaL6fv7E-H5GD3vdIyBcG5wxENTfFqIEzAfiBHHKQMCskKw_2PfyakGmMT5CqZHkpsk9kIjiXmeTskPxdKdf6gJZq5zvX4egMfdza4NfYUq1iUnxPFR0e_egH3247DO5lerm8Z_Pl4nqeQDW6Pt3SwVkat3HEjjY-UKPaPyo4lYzSo2nANfYYEuz7z-Rjo9qI07fzmDxcX60WP2Z3P29uF9_vZoazapxpJTOjJZNKsNwi40rmKLXICwu5hrwyDW-skgAcTcmxqIqGFabILK9yJUpxTE53vkPwvzcYx_rJb0KfVtYcGFSQAcsTdbajTPAxBmzqIbhOhW3NoH6JuV4Uq4vXmK8S_O3NcqM7tHv0PdQEfN0BIZq9-u-fkn7yP70ebCOeAfWkj2s |
Cites_doi | 10.1038/nmat4407 10.1016/S0021-9258(18)31434-0 10.1159/000151372 10.1016/j.biomaterials.2013.12.028 10.1016/S1350-4533(98)00007-1 10.1073/pnas.90.18.8562 10.1002/adfm.201402408 10.1016/j.biomaterials.2015.08.045 10.7150/ijbs.15833 10.1359/jbmr.1998.13.7.1101 10.1359/jbmr.2002.17.10.1822 10.1016/j.jmbbm.2011.08.026 10.1016/j.biomaterials.2010.02.005 10.1359/jbmr.1997.12.12.2040 10.1074/jbc.M305315200 10.1038/nmat945 10.2106/00004623-200212000-00001 10.1002/adma.201302189 10.1038/nrd1695 10.1016/j.bone.2015.08.018 10.1111/j.1582-4934.2010.01224.x 10.1002/jbmr.117 10.1016/j.actbio.2015.04.019 10.1016/j.bbrc.2012.07.037 10.1016/j.bone.2005.11.017 10.1016/j.ejpb.2017.03.016 10.1902/jop.2012.120004 10.1039/C5TB00219B 10.1016/j.mser.2007.05.001 10.1001/jama.2009.956 10.1016/j.joen.2008.02.005 10.1016/j.molcatb.2016.02.003 10.1002/anie.200705238 10.1038/84765 10.1073/pnas.96.11.6020 10.1016/j.matbio.2008.06.004 10.1074/jbc.M212700200 10.1159/000151727 10.1021/bm200274r 10.1038/ijos.2014.44 10.1016/j.joen.2011.05.019 10.1007/s00223-001-1121-z 10.1074/jbc.M309296200 10.1039/C4RA02271H 10.1074/jbc.M113.543330 10.1038/ng1905 10.1007/s11095-014-1299-1 10.1159/000324258 10.1080/03008200390181726 10.1016/S0003-9969(00)00010-8 10.1007/s11999-009-1039-8 10.1371/journal.pone.0102371 10.1073/pnas.0804277105 10.1021/j150521a010 10.1089/ten.tea.2012.0567 10.1074/jbc.M110.194746 10.1074/jbc.M804283200 |
ContentType | Journal Article |
Copyright | Copyright Royal Society of Chemistry 2018 |
Copyright_xml | – notice: Copyright Royal Society of Chemistry 2018 |
DBID | NPM AAYXX CITATION 7QF 7QO 7QQ 7SC 7SE 7SP 7SR 7TA 7TB 7U5 8BQ 8FD F28 FR3 H8D H8G JG9 JQ2 KR7 L7M L~C L~D P64 |
DOI | 10.1039/c7tb02130e |
DatabaseName | PubMed CrossRef Aluminium Industry Abstracts Biotechnology Research Abstracts Ceramic Abstracts Computer and Information Systems Abstracts Corrosion Abstracts Electronics & Communications Abstracts Engineered Materials Abstracts Materials Business File Mechanical & Transportation Engineering Abstracts Solid State and Superconductivity Abstracts METADEX Technology Research Database ANTE: Abstracts in New Technology & Engineering Engineering Research Database Aerospace Database Copper Technical Reference Library Materials Research Database ProQuest Computer Science Collection Civil Engineering Abstracts Advanced Technologies Database with Aerospace Computer and Information Systems Abstracts Academic Computer and Information Systems Abstracts Professional Biotechnology and BioEngineering Abstracts |
DatabaseTitle | PubMed CrossRef Materials Research Database Civil Engineering Abstracts Aluminium Industry Abstracts Technology Research Database Computer and Information Systems Abstracts – Academic Mechanical & Transportation Engineering Abstracts Electronics & Communications Abstracts ProQuest Computer Science Collection Computer and Information Systems Abstracts Ceramic Abstracts Materials Business File METADEX Biotechnology and BioEngineering Abstracts Computer and Information Systems Abstracts Professional Aerospace Database Copper Technical Reference Library Engineered Materials Abstracts Biotechnology Research Abstracts Solid State and Superconductivity Abstracts Engineering Research Database Corrosion Abstracts Advanced Technologies Database with Aerospace ANTE: Abstracts in New Technology & Engineering |
DatabaseTitleList | Materials Research Database CrossRef PubMed |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering |
EISSN | 2050-7518 |
EndPage | 427 |
ExternalDocumentID | 10_1039_C7TB02130E 32254521 c7tb02130e |
Genre | Journal Article |
GroupedDBID | -JG 0-7 705 AAEMU ABGFH ACLDK ADSRN AEFDR AFVBQ AGSTE AUDPV BSQNT C6K EE0 EF- GNO H~N J3I R7C RCNCU RPMJG RRC RSCEA SKA SKF SLH 0R~ 4.4 53G AAIWI AAJAE AANOJ AAWGC AAXHV ABASK ABDVN ABEMK ABJNI ABPDG ABRYZ ABXOH ACGFS ACIWK ACPRK ADMRA AENEX AENGV AESAV AETIL AFLYV AFOGI AFRAH AFRDS AGEGJ AGRSR AHGCF ALMA_UNASSIGNED_HOLDINGS ANUXI APEMP ASKNT BLAPV D0L EBS ECGLT EJD GGIMP H13 HZ~ NPM O-G O9- RAOCF RNS UCJ AAYXX ANBJS AUNWK CITATION J3G J3H ROL 7QF 7QO 7QQ 7SC 7SE 7SP 7SR 7TA 7TB 7U5 8BQ 8FD F28 FR3 H8D H8G JG9 JQ2 KR7 L7M L~C L~D P64 |
ID | FETCH-LOGICAL-c219t-ba54cb515a316de12a56e5b367d06b069cf2fda5002ec82e797f17c74d296a383 |
ISSN | 2050-750X |
IngestDate | Thu Oct 10 22:56:09 EDT 2024 Fri Aug 23 03:18:15 EDT 2024 Sat Sep 28 08:31:19 EDT 2024 Sat Jun 01 02:21:36 EDT 2019 Mon Jan 28 17:15:02 EST 2019 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c219t-ba54cb515a316de12a56e5b367d06b069cf2fda5002ec82e797f17c74d296a383 |
ORCID | 0000-0001-6944-5604 |
PMID | 32254521 |
PQID | 2010904016 |
PQPubID | 2047522 |
PageCount | 14 |
ParticipantIDs | proquest_journals_2010904016 pubmed_primary_32254521 rsc_primary_c7tb02130e crossref_primary_10_1039_C7TB02130E |
ProviderPackageCode | J3I ACLDK RRC AEFDR GNO RCNCU SLH EE0 RSCEA AFVBQ C6K H~N 0-7 RPMJG SKA -JG AGSTE AUDPV EF- BSQNT SKF ADSRN ABGFH 705 AAEMU R7C |
PublicationCentury | 2000 |
PublicationDate | 2018-01-21 |
PublicationDateYYYYMMDD | 2018-01-21 |
PublicationDate_xml | – month: 01 year: 2018 text: 2018-01-21 day: 21 |
PublicationDecade | 2010 |
PublicationPlace | England |
PublicationPlace_xml | – name: England – name: Cambridge |
PublicationTitle | Journal of materials chemistry. B, Materials for biology and medicine |
PublicationTitleAlternate | J Mater Chem B |
PublicationYear | 2018 |
Publisher | Royal Society of Chemistry |
Publisher_xml | – name: Royal Society of Chemistry |
References | Cahill (C7TB02130E-(cit2)/*[position()=1]) 2009; 302 Lin (C7TB02130E-(cit7)/*[position()=1]) 2014; 289 von Marschall (C7TB02130E-(cit12)/*[position()=1]) 2008; 283 Frokjaer (C7TB02130E-(cit25)/*[position()=1]) 2005; 4 Olszta (C7TB02130E-(cit32)/*[position()=1]) 2007; 58 Gan (C7TB02130E-(cit29)/*[position()=1]) 2015; 3 Rittling (C7TB02130E-(cit46)/*[position()=1]) 1998; 13 Zhang (C7TB02130E-(cit36)/*[position()=1]) 2016; 12 Shi (C7TB02130E-(cit38)/*[position()=1]) 2015; 178-189 Alford (C7TB02130E-(cit48)/*[position()=1]) 2006; 38 Wu (C7TB02130E-(cit14)/*[position()=1]) 2011; 286 Prescott (C7TB02130E-(cit56)/*[position()=1]) 2008; 34 Fancy (C7TB02130E-(cit33)/*[position()=1]) 1999; 96 Nanaki (C7TB02130E-(cit45)/*[position()=1]) 2017; 117 Hunter (C7TB02130E-(cit51)/*[position()=1]) 1993; 90 Jiang (C7TB02130E-(cit17)/*[position()=1]) 2010; 25 Siyam (C7TB02130E-(cit20)/*[position()=1]) 2012; 424 Lin (C7TB02130E-(cit8)/*[position()=1]) 2014; 6 Hong (C7TB02130E-(cit57)/*[position()=1]) 2010; 31 Feng (C7TB02130E-(cit21)/*[position()=1]) 2006; 38 Baht (C7TB02130E-(cit50)/*[position()=1]) 2008; 27 Huebsch (C7TB02130E-(cit58)/*[position()=1]) 2015; 14 Du (C7TB02130E-(cit28)/*[position()=1]) 2014; 24 Zhu (C7TB02130E-(cit35)/*[position()=1]) 2011; 22 Zhang (C7TB02130E-(cit49)/*[position()=1]) 2001; 27 Fen (C7TB02130E-(cit6)/*[position()=1]) 2002; 17 Arvidson (C7TB02130E-(cit39)/*[position()=1]) 2011; 15 Xu (C7TB02130E-(cit54)/*[position()=1]) 2011; 12 Hudson (C7TB02130E-(cit27)/*[position()=1]) 2008; 47 Yue (C7TB02130E-(cit41)/*[position()=1]) 2015; 73 Boskey (C7TB02130E-(cit47)/*[position()=1]) 2002; 71 He (C7TB02130E-(cit10)/*[position()=1]) 2003; 2 Rho (C7TB02130E-(cit31)/*[position()=1]) 1998; 20 Zhang (C7TB02130E-(cit34)/*[position()=1]) 2014; 9 Zhou (C7TB02130E-(cit42)/*[position()=1]) 2014; 4 Maciejewska (C7TB02130E-(cit19)/*[position()=1]) 2009; 189 Bhatia (C7TB02130E-(cit23)/*[position()=1]) 2012; 5 Du (C7TB02130E-(cit26)/*[position()=1]) 2013; 25 Balistreri (C7TB02130E-(cit30)/*[position()=1]) 2016; 127 George (C7TB02130E-(cit4)/*[position()=1]) 1993; 268 Tsuji (C7TB02130E-(cit53)/*[position()=1]) 2008; 105 Narayanan (C7TB02130E-(cit18)/*[position()=1]) 2003; 278 Boedtker (C7TB02130E-(cit40)/*[position()=1]) 1954; 58 Sun (C7TB02130E-(cit52)/*[position()=1]) 2013; 19 He (C7TB02130E-(cit11)/*[position()=1]) 2004; 279 Eapen (C7TB02130E-(cit16)/*[position()=1]) 2011; 194 Boraiah (C7TB02130E-(cit1)/*[position()=1]) 2009; 467 Kulkarni (C7TB02130E-(cit13)/*[position()=1]) 2000; 45 Maginot (C7TB02130E-(cit22)/*[position()=1]) 2015; 81 D'Souza (C7TB02130E-(cit5)/*[position()=1]) 1997; 12 Cao (C7TB02130E-(cit43)/*[position()=1]) 2014; 35 He (C7TB02130E-(cit44)/*[position()=1]) 2009; 44 Lu (C7TB02130E-(cit24)/*[position()=1]) 2009; 189 Alsanea (C7TB02130E-(cit55)/*[position()=1]) 2011; 37 Chandrasekaran (C7TB02130E-(cit15)/*[position()=1]) 2013; 84 Wan (C7TB02130E-(cit37)/*[position()=1]) 2014; 31 Qin (C7TB02130E-(cit9)/*[position()=1]) 2003; 278 Govender (C7TB02130E-(cit3)/*[position()=1]) 2002; 84-A |
References_xml | – volume: 14 start-page: 1269 year: 2015 ident: C7TB02130E-(cit58)/*[position()=1] publication-title: Nat. Mater. doi: 10.1038/nmat4407 contributor: fullname: Huebsch – volume: 268 start-page: 12624 year: 1993 ident: C7TB02130E-(cit4)/*[position()=1] publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)31434-0 contributor: fullname: George – volume: 189 start-page: 186 year: 2009 ident: C7TB02130E-(cit19)/*[position()=1] publication-title: Cells Tissues Organs doi: 10.1159/000151372 contributor: fullname: Maciejewska – volume: 22 start-page: 1965 year: 2011 ident: C7TB02130E-(cit35)/*[position()=1] publication-title: J. Mater. Sci.: Mater. Med. contributor: fullname: Zhu – volume: 35 start-page: 2730 year: 2014 ident: C7TB02130E-(cit43)/*[position()=1] publication-title: Biomaterials doi: 10.1016/j.biomaterials.2013.12.028 contributor: fullname: Cao – volume: 20 start-page: 92 year: 1998 ident: C7TB02130E-(cit31)/*[position()=1] publication-title: Med. Eng. Phys. doi: 10.1016/S1350-4533(98)00007-1 contributor: fullname: Rho – volume: 90 start-page: 8562 year: 1993 ident: C7TB02130E-(cit51)/*[position()=1] publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.90.18.8562 contributor: fullname: Hunter – volume: 24 start-page: 7627 year: 2014 ident: C7TB02130E-(cit28)/*[position()=1] publication-title: Adv. Funct. Mater. doi: 10.1002/adfm.201402408 contributor: fullname: Du – volume: 73 start-page: 254 year: 2015 ident: C7TB02130E-(cit41)/*[position()=1] publication-title: Biomaterials doi: 10.1016/j.biomaterials.2015.08.045 contributor: fullname: Yue – volume: 12 start-page: 1203 year: 2016 ident: C7TB02130E-(cit36)/*[position()=1] publication-title: Int. J. Biol. Sci. doi: 10.7150/ijbs.15833 contributor: fullname: Zhang – volume: 13 start-page: 1101 year: 1998 ident: C7TB02130E-(cit46)/*[position()=1] publication-title: J. Bone Miner. Res. doi: 10.1359/jbmr.1998.13.7.1101 contributor: fullname: Rittling – volume: 17 start-page: 1822 year: 2002 ident: C7TB02130E-(cit6)/*[position()=1] publication-title: J. Bone Miner. Res. doi: 10.1359/jbmr.2002.17.10.1822 contributor: fullname: Fen – volume: 5 start-page: 1 year: 2012 ident: C7TB02130E-(cit23)/*[position()=1] publication-title: J. Mech. Behav. Biomed. Mater. doi: 10.1016/j.jmbbm.2011.08.026 contributor: fullname: Bhatia – volume: 31 start-page: 4249e58 year: 2010 ident: C7TB02130E-(cit57)/*[position()=1] publication-title: Biomaterials doi: 10.1016/j.biomaterials.2010.02.005 contributor: fullname: Hong – volume: 12 start-page: 2040 year: 1997 ident: C7TB02130E-(cit5)/*[position()=1] publication-title: J. Bone Miner. Res. doi: 10.1359/jbmr.1997.12.12.2040 contributor: fullname: D'Souza – volume: 278 start-page: 34700 year: 2003 ident: C7TB02130E-(cit9)/*[position()=1] publication-title: J. Biol. Chem. doi: 10.1074/jbc.M305315200 contributor: fullname: Qin – volume: 2 start-page: 552 year: 2003 ident: C7TB02130E-(cit10)/*[position()=1] publication-title: Nat. Mater. doi: 10.1038/nmat945 contributor: fullname: He – volume: 84-A start-page: 2123 year: 2002 ident: C7TB02130E-(cit3)/*[position()=1] publication-title: J. Bone Jt. Surg. Am. Vol. doi: 10.2106/00004623-200212000-00001 contributor: fullname: Govender – volume: 25 start-page: 5981 year: 2013 ident: C7TB02130E-(cit26)/*[position()=1] publication-title: Adv. Mater. doi: 10.1002/adma.201302189 contributor: fullname: Du – volume: 4 start-page: 298 year: 2005 ident: C7TB02130E-(cit25)/*[position()=1] publication-title: Nat. Rev. Drug Discovery doi: 10.1038/nrd1695 contributor: fullname: Frokjaer – volume: 81 start-page: 602 year: 2015 ident: C7TB02130E-(cit22)/*[position()=1] publication-title: Bone doi: 10.1016/j.bone.2015.08.018 contributor: fullname: Maginot – volume: 15 start-page: 718 year: 2011 ident: C7TB02130E-(cit39)/*[position()=1] publication-title: J. Cell. Mol. Med. doi: 10.1111/j.1582-4934.2010.01224.x contributor: fullname: Arvidson – volume: 25 start-page: 2155 year: 2010 ident: C7TB02130E-(cit17)/*[position()=1] publication-title: J. Bone Miner. Res. doi: 10.1002/jbmr.117 contributor: fullname: Jiang – volume: 178-189 start-page: 178 year: 2015 ident: C7TB02130E-(cit38)/*[position()=1] publication-title: Acta Biomater. doi: 10.1016/j.actbio.2015.04.019 contributor: fullname: Shi – volume: 424 start-page: 641 year: 2012 ident: C7TB02130E-(cit20)/*[position()=1] publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2012.07.037 contributor: fullname: Siyam – volume: 38 start-page: 749 year: 2006 ident: C7TB02130E-(cit48)/*[position()=1] publication-title: Bone doi: 10.1016/j.bone.2005.11.017 contributor: fullname: Alford – volume: 117 start-page: 77 year: 2017 ident: C7TB02130E-(cit45)/*[position()=1] publication-title: Eur. J. Pharm. Biopharm. doi: 10.1016/j.ejpb.2017.03.016 contributor: fullname: Nanaki – volume: 84 start-page: 389 year: 2013 ident: C7TB02130E-(cit15)/*[position()=1] publication-title: J. Periodontol. doi: 10.1902/jop.2012.120004 contributor: fullname: Chandrasekaran – volume: 3 start-page: 2281 year: 2015 ident: C7TB02130E-(cit29)/*[position()=1] publication-title: J. Mater. Chem. B doi: 10.1039/C5TB00219B contributor: fullname: Gan – volume: 58 start-page: 77 year: 2007 ident: C7TB02130E-(cit32)/*[position()=1] publication-title: Mater. Sci. Eng., R doi: 10.1016/j.mser.2007.05.001 contributor: fullname: Olszta – volume: 302 start-page: 58 year: 2009 ident: C7TB02130E-(cit2)/*[position()=1] publication-title: JAMA, J. Am. Med. Assoc. doi: 10.1001/jama.2009.956 contributor: fullname: Cahill – volume: 34 start-page: 421 year: 2008 ident: C7TB02130E-(cit56)/*[position()=1] publication-title: J. Endod. doi: 10.1016/j.joen.2008.02.005 contributor: fullname: Prescott – volume: 127 start-page: 26 year: 2016 ident: C7TB02130E-(cit30)/*[position()=1] publication-title: J. Mol. Catal. B: Enzym. doi: 10.1016/j.molcatb.2016.02.003 contributor: fullname: Balistreri – volume: 47 start-page: 8582 year: 2008 ident: C7TB02130E-(cit27)/*[position()=1] publication-title: Angew. Chem., Int. Ed. doi: 10.1002/anie.200705238 contributor: fullname: Hudson – volume: 27 start-page: 151 year: 2001 ident: C7TB02130E-(cit49)/*[position()=1] publication-title: Nat. Genet. doi: 10.1038/84765 contributor: fullname: Zhang – volume: 96 start-page: 6020 year: 1999 ident: C7TB02130E-(cit33)/*[position()=1] publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.96.11.6020 contributor: fullname: Fancy – volume: 27 start-page: 600 year: 2008 ident: C7TB02130E-(cit50)/*[position()=1] publication-title: Matrix Biol. doi: 10.1016/j.matbio.2008.06.004 contributor: fullname: Baht – volume: 278 start-page: 17500 year: 2003 ident: C7TB02130E-(cit18)/*[position()=1] publication-title: J. Biol. Chem. doi: 10.1074/jbc.M212700200 contributor: fullname: Narayanan – volume: 189 start-page: 175 year: 2009 ident: C7TB02130E-(cit24)/*[position()=1] publication-title: Cells Tissues Organs doi: 10.1159/000151727 contributor: fullname: Lu – volume: 12 start-page: 2193 year: 2011 ident: C7TB02130E-(cit54)/*[position()=1] publication-title: Biomacromolecules doi: 10.1021/bm200274r contributor: fullname: Xu – volume: 6 start-page: 133 year: 2014 ident: C7TB02130E-(cit8)/*[position()=1] publication-title: Int. J. Oral Sci. doi: 10.1038/ijos.2014.44 contributor: fullname: Lin – volume: 37 start-page: 1092 year: 2011 ident: C7TB02130E-(cit55)/*[position()=1] publication-title: J. Endod. doi: 10.1016/j.joen.2011.05.019 contributor: fullname: Alsanea – volume: 71 start-page: 145 year: 2002 ident: C7TB02130E-(cit47)/*[position()=1] publication-title: Calcif. Tissue Int. doi: 10.1007/s00223-001-1121-z contributor: fullname: Boskey – volume: 279 start-page: 11649 year: 2004 ident: C7TB02130E-(cit11)/*[position()=1] publication-title: J. Biol. Chem. doi: 10.1074/jbc.M309296200 contributor: fullname: He – volume: 4 start-page: 21997 year: 2014 ident: C7TB02130E-(cit42)/*[position()=1] publication-title: RSC Adv. doi: 10.1039/C4RA02271H contributor: fullname: Zhou – volume: 289 start-page: 21533 year: 2014 ident: C7TB02130E-(cit7)/*[position()=1] publication-title: J. Biol. Chem. doi: 10.1074/jbc.M113.543330 contributor: fullname: Lin – volume: 38 start-page: 1310 year: 2006 ident: C7TB02130E-(cit21)/*[position()=1] publication-title: Nat. Genet. doi: 10.1038/ng1905 contributor: fullname: Feng – volume: 31 start-page: 1967 year: 2014 ident: C7TB02130E-(cit37)/*[position()=1] publication-title: Pharm. Res. doi: 10.1007/s11095-014-1299-1 contributor: fullname: Wan – volume: 194 start-page: 255 year: 2011 ident: C7TB02130E-(cit16)/*[position()=1] publication-title: Cells Tissues Organs doi: 10.1159/000324258 contributor: fullname: Eapen – volume: 44 start-page: 240 year: 2009 ident: C7TB02130E-(cit44)/*[position()=1] publication-title: Connect. Tissue Res. doi: 10.1080/03008200390181726 contributor: fullname: He – volume: 45 start-page: 475 year: 2000 ident: C7TB02130E-(cit13)/*[position()=1] publication-title: Arch. Oral Biol. doi: 10.1016/S0003-9969(00)00010-8 contributor: fullname: Kulkarni – volume: 467 start-page: 3257 year: 2009 ident: C7TB02130E-(cit1)/*[position()=1] publication-title: Clin. Orthop. Relat. Res. doi: 10.1007/s11999-009-1039-8 contributor: fullname: Boraiah – volume: 9 start-page: e102371 year: 2014 ident: C7TB02130E-(cit34)/*[position()=1] publication-title: PLoS One doi: 10.1371/journal.pone.0102371 contributor: fullname: Zhang – volume: 105 start-page: 16866 year: 2008 ident: C7TB02130E-(cit53)/*[position()=1] publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.0804277105 contributor: fullname: Tsuji – volume: 58 start-page: 968 year: 1954 ident: C7TB02130E-(cit40)/*[position()=1] publication-title: J. Phys. Chem. doi: 10.1021/j150521a010 contributor: fullname: Boedtker – volume: 19 start-page: 1754 year: 2013 ident: C7TB02130E-(cit52)/*[position()=1] publication-title: Tissue Eng., Part A doi: 10.1089/ten.tea.2012.0567 contributor: fullname: Sun – volume: 286 start-page: 29462 year: 2011 ident: C7TB02130E-(cit14)/*[position()=1] publication-title: J. Biol. Chem. doi: 10.1074/jbc.M110.194746 contributor: fullname: Wu – volume: 283 start-page: 32730 year: 2008 ident: C7TB02130E-(cit12)/*[position()=1] publication-title: J. Biol. Chem. doi: 10.1074/jbc.M804283200 contributor: fullname: von Marschall |
SSID | ssj0000816834 |
Score | 2.1295838 |
Snippet | Searching for effective osteoinduction factors with higher specificity and biosafety for the preparation of biomimetic materials, which mimic the natural bone... |
SourceID | proquest crossref pubmed rsc |
SourceType | Aggregation Database Index Database Enrichment Source Publisher |
StartPage | 414 |
SubjectTerms | Alkaline phosphatase Biocompatibility Biomarkers Biomedical materials Biomimetic materials Biomimetics Bone growth Bone marrow Bone matrix Cbfa-1 protein Dentin Dmp1 protein Embedded systems Extracellular matrix Gelatin Hybrid systems Hydrogels In vivo methods and tests Kinetics Mineralization Nodules Osteogenesis Pore size Pore size distribution Porosity Regeneration Regeneration (physiology) Silica Silicon dioxide Size distribution Stem cell transplantation Stem cells Structural hierarchy Synergistic effect |
Title | Tailored biomimetic hydrogel based on a photopolymerised DMP1/MCF/gelatin hybrid system for calvarial bone regeneration |
URI | https://www.ncbi.nlm.nih.gov/pubmed/32254521 https://www.proquest.com/docview/2010904016 |
Volume | 6 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Jb9NAFB6F9AIHxFZwKWgkuEVu7PEy8bFNUxXUIBCpyM2axU4i0TgKDhB-Hr-MN4sXkh4KFyt6jiexv8_z3pt5C0Jvia9a4kjiypgyFya83OWh8FwS83wwABUhpNrRHX-IL6_D99No2un8bkUtbUp-In7dmlfyP6iCDHBVWbL_gGw9KAjgM-ALR0AYjnfDmC3A3VY25KK4WdyofMTefCvXxSz72lP6Saq9ANZbzYtSNUPYqu0ZJT0ff1Qz2Hh4AceZjodbwpUqe8vWdtbhh4Dfd6bbevBiqfqrzHSR6hrLfaMW7F9z4z1RdZI76Z2ZpKDqjA4QbdV-2t3evzJlDT7PNz9b3B2ywq4hzLaN9Itd7v7U-ub5RlNzweabRXtRw1fxdK7JlDZzH_EizwVjZmrUVFtmp2s7ecctjgatiTg0qalWp4em_sCeuvACVW11SCdnYOoE3qhRilUgwI6urCMY9d59kKTNtffQAaFJFHXRwelo8u6qXunTrU10eEN9V1Wd3CDpNwP8bRntuTtg_KyrpjTa-Jk8Qg8twPjUUPAx6mTLJ-hBq5blU_SjIiNuyIgrMmJNRlwsMcO7ZMSKjH2gYt8SERsiYkNEDHTBNRGxIiJuE_EZur4YTYaXru3q4QrQjqXLWRQKDmY0C_xYZj5hUZxFPIip9GLuxYnISS5Vow6SiQHJaEJznwoaSpLELBgEh6i7hN96gTAlkglfgotAZEi1N0FVxtMg4eDV0NBBb6onmq5M8ZZ0HzgHHVcPO7Uv97eU6IjlEBwiBz03ANRDKB0YgtnroENApBYLWnI9Yuago9tPpCuZH93pT71E95v34hh1y_UmewXmb8lfW379AW-ZsMg |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | Royal Society of Chemistry |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Tailored+biomimetic+hydrogel+based+on+a+photopolymerised+DMP1%2FMCF%2Fgelatin+hybrid+system+for+calvarial+bone+regeneration&rft.jtitle=Journal+of+materials+chemistry.+B%2C+Materials+for+biology+and+medicine&rft.au=Lin%2C+Shuxian&rft.au=Cao%2C+Lingyan&rft.au=Wang%2C+Qian&rft.au=Du%2C+Jiahui&rft.date=2018-01-21&rft.issn=2050-750X&rft.eissn=2050-7518&rft.volume=6&rft.issue=3&rft.spage=414&rft.epage=427&rft_id=info:doi/10.1039%2FC7TB02130E&rft.externalDBID=n%2Fa&rft.externalDocID=10_1039_C7TB02130E |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2050-750X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2050-750X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2050-750X&client=summon |