In situ fixation of cultured mouse peritoneal exudate cells: comparison of fixation methods

• Published in: In vitro Vol. 16; no. 2; p. 136
• Main Authors: Morris, R E, Ciraolo, G M, Cohen, D A, Bubel, H C
• Format: Journal Article
• Language: English
• Published: United States 01.02.1980
• Subjects: Animals; Ascitic Fluid - cytology; Buffers; Cell Membrane - ultrastructure; Cells, Cultured; Cytological Techniques; Cytoplasm - ultrastructure; Fixatives; Glutaral; Mice; Organoids - ultrastructure; Osmium Tetroxide; Temperature

Mouse peritoneal exudate cells grown in vitro on plastic petri dishes were fixed in situ with both glutaraldehyde and osmium tetroxide by a variety of contemporary methods. The goal of the investigation was to determine which method resulted in the best ultrastructural preservation. The parameters b...