Reversible neuromuscular blocking action of carbohymethylated α-bungarotoxin

Carbanidomethylation of the reduced Cys 29-Cys 33 bridge of α-bungarotoxin ( Bungarus multicinctus postsynaptic neurotoxin) did not alter the LD 50 or irreversibility of the toxin, while carboxymethylated α-bungarotoxin was less potent and its neuromuscular blocking action in mouse diaphragm was rev...

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Published inBiochimica et biophysica acta. General subjects Vol. 755; no. 2; pp. 210 - 216
Main Authors Chewn-Lang Ho, Shih-Hsiung Wu, Charng-Jui Chen
Format Journal Article
LanguageEnglish
Published Elsevier B.V 25.01.1983
Subjects
Acetylcholine receptor
Bungarotoxin
Carboxymethylation
Circular dichroism
Neurotoxin
Carboxymethylation
Bungarotoxin
Acetylcholine receptor
Neurotoxin
Circular dichroism
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Abstract Carbanidomethylation of the reduced Cys 29-Cys 33 bridge of α-bungarotoxin ( Bungarus multicinctus postsynaptic neurotoxin) did not alter the LD 50 or irreversibility of the toxin, while carboxymethylated α-bungarotoxin was less potent and its neuromuscular blocking action in mouse diaphragm was reversible. The circular dichroic spectra of both modified toxins were similar but slightly different from that of native toxin. Neuromuscular transmission in the chick biventer cervicis nerve-muscle preparation could be blocked by the carboxymethylated toxin, and reactivated by washing, whereas the response of the muscle to extrinsic acetylcholine could also be blocked but was hardly restored by washing. These results suggest that carboxymethylated toxin can differentiate between junctional and extrajunctional acetylcholine receptors in chick skeletal muscle.
AbstractList Carbanidomethylation of the reduced Cys 29-Cys 33 bridge of α-bungarotoxin ( Bungarus multicinctus postsynaptic neurotoxin) did not alter the LD 50 or irreversibility of the toxin, while carboxymethylated α-bungarotoxin was less potent and its neuromuscular blocking action in mouse diaphragm was reversible. The circular dichroic spectra of both modified toxins were similar but slightly different from that of native toxin. Neuromuscular transmission in the chick biventer cervicis nerve-muscle preparation could be blocked by the carboxymethylated toxin, and reactivated by washing, whereas the response of the muscle to extrinsic acetylcholine could also be blocked but was hardly restored by washing. These results suggest that carboxymethylated toxin can differentiate between junctional and extrajunctional acetylcholine receptors in chick skeletal muscle.
Author Chewn-Lang Ho
Charng-Jui Chen
Shih-Hsiung Wu
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Keywords Carboxymethylation
Bungarotoxin
Acetylcholine receptor
Neurotoxin
Circular dichroism
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References Chang, Su (BIB16) 1975; 33
Chang, Lee (BIB1) 1963; 144
Chang, Su, Tung (BIB17) 1977; 268
Chang, Tang (BIB15) 1974; 282
Mebs, Narita, Iwanaga, Samejima, Lee (BIB19) 1971; 44
Lee (BIB13) 1972; 12
Botes (BIB5) 1971; 26
Ginsborg, Warriner (BIB10) 1960; 15
Lee, Chang, Chen (BIB14) 1972; 71
Albuguergue, Eldefrawi, Eldefrawi (BIB4) 1979; Vol. 52
Lee, Chang, Kau, Luh (BIB8) 1972; 72
Narahashi (BIB3) 1974; 54
Chicheportiche, Vincent, Kopeyan, Schweitz, Lazdunski (BIB7) 1975; 14
Chang, Brauer, Wittmann-Leibold (BIB9) 1978; 93
Botes (BIB6) 1974; 359
Litchfield, Wilcoxon (BIB12) 1949; 96
Changeux, Kakai, Lee (BIB2) 1970; 67
Bülbring (BIB11) 1946; 1
Visser, Louw (BIB18) 1978; 533
References_xml – volume: 33
  start-page: 337
  year: 1975
  end-page: 344
  ident: BIB16
  publication-title: Eur. J. Pharmacol.
– volume: 72
  start-page: 71
  year: 1972
  end-page: 82
  ident: BIB8
  publication-title: J. Chromatog.
– volume: 533
  start-page: 80
  year: 1978
  end-page: 89
  ident: BIB18
  publication-title: Biochim. Biophys. Acta.
– volume: 282
  start-page: 379
  year: 1974
  end-page: 388
  ident: BIB15
  publication-title: Naunyn-Schmiedeberg's Arch. Pharmacol.
– volume: 359
  start-page: 242
  year: 1974
  end-page: 247
  ident: BIB6
  publication-title: Biochim. Biophys. Acta
– volume: 268
  start-page: 449
  year: 1977
  end-page: 465
  ident: BIB17
  publication-title: J. Physiol.
– volume: 144
  start-page: 241
  year: 1963
  end-page: 257
  ident: BIB1
  publication-title: Arch. Int. Pharmacodyn.
– volume: 96
  start-page: 99
  year: 1949
  end-page: 113
  ident: BIB12
  publication-title: J. Pharmacol. Exp. Ther.
– volume: 93
  start-page: 205
  year: 1978
  end-page: 214
  ident: BIB9
  publication-title: FEBS Lett.
– volume: 15
  start-page: 418
  year: 1960
  end-page: 425
  ident: BIB10
  publication-title: Br. J. Pharmacol.
– volume: 44
  start-page: 711
  year: 1971
  end-page: 716
  ident: BIB19
  publication-title: Biochem. Biophys. Res. Commun.
– volume: 1
  start-page: 38
  year: 1946
  end-page: 61
  ident: BIB11
  publication-title: Br. J. Pharmacol.
– volume: 12
  start-page: 265
  year: 1972
  end-page: 286
  ident: BIB13
  publication-title: Annu. Rev. Pharmacol.
– volume: 71
  start-page: 344
  year: 1972
  end-page: 349
  ident: BIB14
  publication-title: J. Formosan Med. Assoc.
– volume: Vol. 52
  start-page: 377
  year: 1979
  end-page: 402
  ident: BIB4
  publication-title: Handbook of Experimental Pharmacology
– volume: 67
  start-page: 1241
  year: 1970
  end-page: 1274
  ident: BIB2
  publication-title: Proc. Natl. Acad. Sci. U.S.A.
– volume: 14
  start-page: 2081
  year: 1975
  end-page: 2091
  ident: BIB7
  publication-title: Biochemistry
– volume: 26
  start-page: 7383
  year: 1971
  end-page: 7391
  ident: BIB5
  publication-title: J. Biol. Chem.
– volume: 54
  start-page: 813
  year: 1974
  end-page: 889
  ident: BIB3
  publication-title: Physiol. Rev.
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Snippet Carbanidomethylation of the reduced Cys 29-Cys 33 bridge of α-bungarotoxin ( Bungarus multicinctus postsynaptic neurotoxin) did not alter the LD 50 or...
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StartPage 210
SubjectTerms Acetylcholine receptor
Bungarotoxin
Carboxymethylation
Circular dichroism
Neurotoxin
Title Reversible neuromuscular blocking action of carbohymethylated α-bungarotoxin
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