Histological studies on cultured canine heart valves recovered from −196 °C

• Published in: Cryobiology Vol. 14; no. 1; pp. 15 - 22
• Main Authors: Rajotte, R.V., Shnitka, T.K., Liburd, E.M., Dossetor, J.B., Voss, W.A.G.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 1977

Adult canine heart valves have been frozen to −196 °C, (0.5 to 0.7 °C/min from 0 to −100 °C) with 10% DMSO ( v v ), stored, thawed at ~150 °C/min, and then cultured for 9 to 12 days. A histological analysis of sections derived from several valves indicates viability, but with a not inconsiderable lo...