GROWTH-INHIBITORY EFFECTS OF CURCUMIN ON Raji CELLS AND ITS MECHANISMS

• Published in: Chinese journal of cancer research Vol. 17; no. 3; pp. 203 - 206
• Main Author: 陈燕 吴青 李新刚
• Format: Journal Article
• Language: English
• Published: Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022 01.09.2005
• Subjects: 基因表达; 姜黄色素; 抗癌基因; 生长抑制因子; 细胞凋亡; Curcumin; Raji cell; Gene expression; Apoptosis
• ISSN: 1000-9604; 1993-0631
• DOI: 10.1007/s11670-005-0041-2

Objective： To study the growth-inhibitory effects of curcumin on B-NHL cell line Raji cells in vitro and its molecular mechanisms. Methods： The growth inhibition rates of Raji cells, after being treated with 6.25μmol/L - 50μmol/L curcumin for 12 h - 48 h, were examined by MTT assay. The apoptosis rate was detected by flow cytometry （FCM）, the protein expression levels of bcl-2 and p53 in Raji cells were examined by SP immunohistochemistry. The expression of p53 in Raji cell were checked by RT-PCR. Results： After being treated by various concentrations of curcumin, the growth of Raji cells was inhibited significantly. The rates of apoptosis were 11.8% -79.7% （P〈0.01）, the down regulation of p53 expression was observed within 24 h after the treatment of curcumin by RT-PCR. The expression of bcl-2 and p53 was decreased, which depended on the action time. Conclusion： Curcumin could significantly inhibit the growth of Raji cells. The induction of apoptosis by down-regulating the expression of bcl-2 and p53 was probably one of its molecular mechanisms.