Effect of Colchicine on the Induction of Prunella vulgaris for. albiflora Nakai
This study was conducted to find out the effective induction method of tetraploid plants to obtain potential data for cultivating superior varieties by colchicine treatment. The seed germination were decreased by the higher concentration of colchicine treatment and longer soaking time. A total of 90...
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Published in | Korean journal of crop science Vol. 60; no. 1; pp. 107 - 113 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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한국작물학회
31.03.2015
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Abstract | This study was conducted to find out the effective induction method of tetraploid plants to obtain potential data for cultivating superior varieties by colchicine treatment. The seed germination were decreased by the higher concentration of colchicine treatment and longer soaking time. A total of 907 individuals were germinated in 16 treated plots except control (untreated plot) and 28 tetraploids were induced which was about 3.1% of the number of seed germinated. The plant regeneration rate by colchicine treatment on explant of Prunella vulgaris for. albiflora Nakai under in vitro culture was decreased with the higher concentration of colchicine. While a total of 312 individuals were regenerated in all treatments, the explant was soaked in more than 0.05% for over 1 hour, tetraploid could be obtained. In particular, for the soaking treatment in 0.05% for 6 hours and 12 hours, 37 tetraploids were induced, which was about 57.8% of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order to identify polyploid, the peak DNA content of G1 phase was 101.3 for diploid and 197.2 for tetraploid. The result confirmed the doubling of DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploid and 19.3 in tetraploid, which was around 1.9 times as much as diploid. |
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AbstractList | This study was conducted to find out the effectiveinduction method of tetraploid plants to obtain potential datafor cultivating superior varieties by colchicine treatment. Theseed germination were decreased by the higher concentrationof colchicine treatment and longer soaking time. A total of907 individuals were germinated in 16 treated plots exceptcontrol (untreated plot) and 28 tetraploids were induced whichwas about 3.1% of the number of seed germinated. The plantregeneration rate by colchicine treatment on explant of Prunellavulgaris for. albiflora Nakai under in vitro culture was decreasedwith the higher concentration of colchicine. While a total of312 individuals were regenerated in all treatments, the explantwas soaked in more than 0.05% for over 1 hour, tetraploidcould be obtained. In particular, for the soaking treatmentin 0.05% for 6 hours and 12 hours, 37 tetraploids wereinduced, which was about 57.8% of the number of plantregenerated. In accordance with the observation on doublingof DNA contents in leaf in order to identify polyploid, thepeak DNA content of G1 phase was 101.3 for diploid and197.2 for tetraploid. The result confirmed the doubling ofDNA content. Furthermore, the number of chloroplasts per guardcell depending on polyploid was around 10 in diploid and19.3 in tetraploid, which was around 1.9 times as much asdiploid.
Keywords : tetraploid, colchicine, DNA content, number ofchloroplasts, morphological characteristic KCI Citation Count: 2 This study was conducted to find out the effective induction method of tetraploid plants to obtain potential data for cultivating superior varieties by colchicine treatment. The seed germination were decreased by the higher concentration of colchicine treatment and longer soaking time. A total of 907 individuals were germinated in 16 treated plots except control (untreated plot) and 28 tetraploids were induced which was about 3.1% of the number of seed germinated. The plant regeneration rate by colchicine treatment on explant of Prunella vulgaris for. albiflora Nakai under in vitro culture was decreased with the higher concentration of colchicine. While a total of 312 individuals were regenerated in all treatments, the explant was soaked in more than 0.05% for over 1 hour, tetraploid could be obtained. In particular, for the soaking treatment in 0.05% for 6 hours and 12 hours, 37 tetraploids were induced, which was about 57.8% of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order to identify polyploid, the peak DNA content of G1 phase was 101.3 for diploid and 197.2 for tetraploid. The result confirmed the doubling of DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploid and 19.3 in tetraploid, which was around 1.9 times as much as diploid. |
Author | Kwon, S.J., Woosong College, Daejeon, Republic of Korea Roy, S.K., Chungbuk National University, Cheongju, Republic of Korea Kim, H.H., Woosong College, Daejeon, Republic of Korea Woo, S.H., Chungbuk National University, Cheongju, Republic of Korea Cho, K.Y., Woosong College, Daejeon, Republic of Korea Moon, Y.J., Woosong College, Daejeon, Republic of Korea |
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CitedBy_id | crossref_primary_10_7732_kjpr_2016_29_3_347 crossref_primary_10_2478_fhort_2023_0003 crossref_primary_10_1007_s12892_018_0096_0 crossref_primary_10_3390_ijms241914659 |
Cites_doi | 10.7732/kjpr.2013.26.2.284 10.1038/1801209a0 10.1038/221865a0 10.2135/cropsci1975.0011183X001500060006x 10.2135/cropsci1966.0011183X000600020036x 10.1016/S0304-4238(99)00077-1 10.1007/BF00022632 10.1139/g75-073 10.1007/BF00405195 10.1126/science.220.4601.1049 10.2134/agronj1958.00021962005000030016x 10.21273/JASHS.101.6.671 10.1007/BF01275438 10.1007/BF02853666 |
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CorporateAuthor | Department of Food Nutrition and Cookery, Woosong College, Daejeon 300-715, Korea |
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References | (JMHHBK_2015_v60n1_107_002) 1991; 68 (JMHHBK_2015_v60n1_107_008) 1958; 50 (JMHHBK_2015_v60n1_107_007) 1976; 101 (JMHHBK_2015_v60n1_107_015) 2013; 26 (JMHHBK_2015_v60n1_107_016) 1975; 17 (JMHHBK_2015_v60n1_107_010) 1983; 220 (JMHHBK_2015_v60n1_107_003) 1969; 221 (JMHHBK_2015_v60n1_107_017) 1996; 16 (JMHHBK_2015_v60n1_107_018) 1966; 6 (JMHHBK_2015_v60n1_107_020) 1999; 82 (JMHHBK_2015_v60n1_107_014) 2003; 6 (JMHHBK_2015_v60n1_107_013) 1957; 180 (JMHHBK_2015_v60n1_107_011) 1983; 157 (JMHHBK_2015_v60n1_107_021) 1973; 22 (JMHHBK_2015_v60n1_107_001) 2001; 28 (JMHHBK_2015_v60n1_107_004) 1987; 30 (JMHHBK_2015_v60n1_107_012) 1969; 5 (JMHHBK_2015_v60n1_107_006) 1994; 26 (JMHHBK_2015_v60n1_107_009) 1926; 12 (JMHHBK_2015_v60n1_107_019) 1994; 36 (JMHHBK_2015_v60n1_107_005) 1975; 15 |
References_xml | – volume: 26 start-page: 284 year: 2013 ident: JMHHBK_2015_v60n1_107_015 publication-title: Korean J. Plant Res. doi: 10.7732/kjpr.2013.26.2.284 – volume: 180 start-page: 1209 year: 1957 ident: JMHHBK_2015_v60n1_107_013 publication-title: Nature doi: 10.1038/1801209a0 – volume: 28 start-page: 179 year: 2001 ident: JMHHBK_2015_v60n1_107_001 publication-title: Korean J. Plant Tissue Culture – volume: 6 start-page: 188 year: 2003 ident: JMHHBK_2015_v60n1_107_014 publication-title: Korean J. Plant Res. – volume: 221 start-page: 865 year: 1969 ident: JMHHBK_2015_v60n1_107_003 publication-title: Nature doi: 10.1038/221865a0 – volume: 36 start-page: 1 year: 1994 ident: JMHHBK_2015_v60n1_107_019 publication-title: Kor. J. Seric. Sci. – volume: 15 start-page: 760 year: 1975 ident: JMHHBK_2015_v60n1_107_005 publication-title: Crop Sci doi: 10.2135/cropsci1975.0011183X001500060006x – volume: 5 start-page: 48 year: 1969 ident: JMHHBK_2015_v60n1_107_012 publication-title: J. Kor. Soc. Hort. Sci. – volume: 12 start-page: 373 year: 1926 ident: JMHHBK_2015_v60n1_107_009 publication-title: Amer. J. Bot. – volume: 6 start-page: 213 year: 1966 ident: JMHHBK_2015_v60n1_107_018 publication-title: Crop Sci doi: 10.2135/cropsci1966.0011183X000600020036x – volume: 82 start-page: 265 year: 1999 ident: JMHHBK_2015_v60n1_107_020 publication-title: Sci. Hort. doi: 10.1016/S0304-4238(99)00077-1 – volume: 22 start-page: 244 year: 1973 ident: JMHHBK_2015_v60n1_107_021 publication-title: Euphytica doi: 10.1007/BF00022632 – volume: 17 start-page: 591 year: 1975 ident: JMHHBK_2015_v60n1_107_016 publication-title: Can. J. Genet. Cytol. doi: 10.1139/g75-073 – volume: 30 start-page: 158 year: 1987 ident: JMHHBK_2015_v60n1_107_004 publication-title: Genome – volume: 157 start-page: 278 year: 1983 ident: JMHHBK_2015_v60n1_107_011 publication-title: Planta doi: 10.1007/BF00405195 – volume: 220 start-page: 1049 year: 1983 ident: JMHHBK_2015_v60n1_107_010 publication-title: Science doi: 10.1126/science.220.4601.1049 – volume: 50 start-page: 169 year: 1958 ident: JMHHBK_2015_v60n1_107_008 publication-title: Agron. J. doi: 10.2134/agronj1958.00021962005000030016x – volume: 101 start-page: 671 year: 1976 ident: JMHHBK_2015_v60n1_107_007 publication-title: J. Amer. Soc. Hort. Sci. doi: 10.21273/JASHS.101.6.671 – volume: 26 start-page: 66 year: 1994 ident: JMHHBK_2015_v60n1_107_006 publication-title: Korean J. Breed. – volume: 16 start-page: 1 year: 1996 ident: JMHHBK_2015_v60n1_107_017 publication-title: Plant Cell Rep doi: 10.1007/BF01275438 – volume: 68 start-page: 279 year: 1991 ident: JMHHBK_2015_v60n1_107_002 publication-title: Am. Potato J. doi: 10.1007/BF02853666 |
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Snippet | This study was conducted to find out the effective induction method of tetraploid plants to obtain potential data for cultivating superior varieties by... This study was conducted to find out the effectiveinduction method of tetraploid plants to obtain potential datafor cultivating superior varieties by... |
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StartPage | 107 |
SubjectTerms | COLCHICINA COLCHICINE DNA content,number of chloroplasts,morphological characteristic TETRAPLOIDIA TETRAPLOIDIE TETRAPLOIDY 농학 |
TableOfContents | MATERIALS AND METHODS Tetraploid cultivation by colchicine treatment DNA content analysis using cytometry RESULTS AND DISCUSSION Seed soaking treatment of colchicine In vitro cultured explant soaking treatment of colchicine REFERENCES |
Title | Effect of Colchicine on the Induction of Prunella vulgaris for. albiflora Nakai |
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