Expression of Mouse Telomerase Catalytic Subunit mTERT Gene in Testis of SD Rats and Its Significance

• Published in: Current medical science Vol. 23; no. 3; pp. 288 - 290
• Main Author: 叶哲伟 陈晓春 杨述华 陈江 熊雅丽 鲁功成
• Format: Journal Article
• Language: English
• Published: China Department of Orthopedics, Xiehe hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030%Department of Urology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology. Wuhah 430030%Department of Obstetrics and Gynecology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030 2003
• Subjects: Animals; Catalysis; Cell Differentiation; DNA-Binding Proteins; Gene Expression Regulation, Developmental; Male; mTERT基因; Rats; Rats, Sprague-Dawley; RNA; Spermatids - enzymology; Spermatids - ultrastructure; Spermatogenesis - genetics; Spermatogonia - enzymology; Spermatogonia - ultrastructure; Spermatozoa - enzymology; Spermatozoa - ultrastructure; Telomerase - biosynthesis; Telomerase - genetics; Telomerase - metabolism; Testis - enzymology; Testis - growth & development; 原位杂交; 基因表达; 端粒酶; 老年性痴呆; germ cell; gene expression; telomerase: in situ hybridization
• ISSN: 1672-0733; 2096-5230; 2523-899X
• DOI: 10.1007/bf02829517

To study the expression of mTERT gene in the testis of SD rats and its significance, in situ hybridization (ISH) techniques were used to detect the expression of telomerase gene mTERT mRNA in the testis of SD rats. The expression of mTERT was detectable in different-age male SD rats" testis. There was a positive correlation between the expression of mTERT and the location of germ cells (spermatogonia, spermatocyte, spermatid). In Sertoli cells, leydig cell and spermatozoa, telomerase mTERT was not detected. Type A spermatogonia expressed the highest level of telomerase mTERT mRNA. Our results suggest that the expression of mTERT gene in the testis of SD rats is of lifetime and coincide with the telomerase activity.