A Sensitive Liquid Chromatography-Mass Spectrometric Method for Determination of Bisoprolol in Rat Serum after Pre-Column Derivatization

• Published in: Journal of chromatographic science Vol. 62; no. 7; p. 668
• Main Authors: Shaheen, Huda A, Bahaffi, Saleh O, Khedr, Alaa M
• Format: Journal Article
• Language: English
• Published: United States 20.08.2024
• Subjects: Animals; Bisoprolol - blood; Bisoprolol - chemistry; Bisoprolol - pharmacokinetics; Chromatography, Liquid - methods; Limit of Detection; Linear Models; Male; Mass Spectrometry - methods; Rats; Rats, Sprague-Dawley; Reproducibility of Results; LC-MS; derivatization; bisoprolol; pharmacokinetic; 3-bromomethyl-propyphenazone

Two derivatization regents were reacted with bisoprolol (BIS), followed by liquid-chromatography-mass spectrometric analysis. 3-Bromomethyl-propyphenazone (BMP) and dansyl-chloride (Dns-Cl) were reacted via the secondary amino group using a catalyst to accelerate the reaction progress and completene...