Glycoprotein enrichment through lectin affinity techniques

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 424; p. 373
• Main Authors: Mechref, Yehia, Madera, Milan, Novotny, Milos V
• Format: Journal Article
• Language: English
• Published: United States 2008
• Subjects: Biomarkers - analysis; Biomarkers - chemistry; Cardiovascular Diseases - metabolism; Chromatography, Affinity - methods; Genetic Diseases, Inborn - metabolism; Genome, Human; Glycoproteins - analysis; Glycoproteins - chemistry; Glycoproteins - isolation & purification; Glycoproteins - metabolism; Glycosylation; Humans; Immune System Diseases - metabolism; Lectins - chemistry; Microfluidic Analytical Techniques - methods; Neoplasms - metabolism; Neurodegenerative Diseases - metabolism; Protein Processing, Post-Translational

Posttranslational modifications (PTM) of proteins are among the key biological regulators of function, activity, localization, and interaction. The fact that no more than 30,000-50,000 proteins are encoded by the human genome underlines the importance of posttranslational modifications in modulating...