Effect of Copper on the Uptake of Carbon from Medium to Daphnia magna
Effect of Cu on the carbon transfer from medium to Daphnia magna has been studied. The concentration of Cu in the medium containing mixed algae was 0.1 ppm, 0.3 ppm and 0.5 ppm. The 13C added in the medium was 20 mg/50mL as NaH13CO3. The temperature in the room used for incubation was controlled at...
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Published in | RADIOISOTOPES Vol. 54; no. 7; pp. 225 - 228 |
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Main Authors | , , , , |
Format | Journal Article |
Language | Japanese English |
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Japan Radioisotope Association
2005
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Abstract | Effect of Cu on the carbon transfer from medium to Daphnia magna has been studied. The concentration of Cu in the medium containing mixed algae was 0.1 ppm, 0.3 ppm and 0.5 ppm. The 13C added in the medium was 20 mg/50mL as NaH13CO3. The temperature in the room used for incubation was controlled at 24°C and the light intensity at the medium (in 50 mL beaker) was about 3000 lux (14 hours: light, 10 hours : dark) . After 24 hour incubation, The Daphnia magna and the phytoplankton were dried and 13C abundance of each sample were measured by a 13CO2 analyzer. The 13C abundance in Daphnia magna were 2.9 atom% excess in control, 1.1 atom% excess in 0.1 ppm, 0.6 atom% excess in 0.3 ppm and 0.6 atom% excess in 0.5 ppm, respectively. And in phytoplankton, they were 6.7 atom% excess in control, 6.5 atom% excess in 0.1 ppm, 6.1 atom% excess in 0.3 ppm and 4.0 atom% excess in 0.5 ppm. The 13C abundance in phytoplankton was not so decreased as in Daphnia magna. The lower 13C abundance observed in Daphnia magna were mainly caused by the less intake of phytoplankton derived by the Cu in the medium. |
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AbstractList | Effect of Cu on the carbon transfer from medium to Daphnia magna has been studied. The concentration of Cu in the medium containing mixed algae was 0.1 ppm, 0.3 ppm and 0.5 ppm. The 13C added in the medium was 20 mg/50mL as NaH13CO3. The temperature in the room used for incubation was controlled at 24°C and the light intensity at the medium (in 50 mL beaker) was about 3000 lux (14 hours: light, 10 hours : dark) . After 24 hour incubation, The Daphnia magna and the phytoplankton were dried and 13C abundance of each sample were measured by a 13CO2 analyzer. The 13C abundance in Daphnia magna were 2.9 atom% excess in control, 1.1 atom% excess in 0.1 ppm, 0.6 atom% excess in 0.3 ppm and 0.6 atom% excess in 0.5 ppm, respectively. And in phytoplankton, they were 6.7 atom% excess in control, 6.5 atom% excess in 0.1 ppm, 6.1 atom% excess in 0.3 ppm and 4.0 atom% excess in 0.5 ppm. The 13C abundance in phytoplankton was not so decreased as in Daphnia magna. The lower 13C abundance observed in Daphnia magna were mainly caused by the less intake of phytoplankton derived by the Cu in the medium. |
Author | YANAGISAWA, Kei ISHII, Nobuyoshi MIYAMOTO, Kiriko FUMA, Shoichi TAKEDA, Hiroshi |
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References | 1) Puma, S., Ishii, N., Takeda, H., Miyamoto, K., Yanagisawa, K., Ichimasa, Y., Saito, M. and Kawabata, Z., Characterization of simple aquatic microcosm for ecotoxicity screening, Jpn. J. Environ. Toxicol., 5, 51-63 (2002 2) Yanagisawa, K., Takeda, H., Miyamoto, K., Fuma, S., Ishii, N. and Tanaka, N., Determination of 13C concentration in biological materials using infrared absorption method, J. Radioanal. Nucl. Chem., 252, 227-280 (2002 3) 柳澤啓, 武田洋, 宮本霧子, 府馬正一, 石井伸昌, 赤外線吸収法による微量試料中の13C分析, RADIOISOTOPES, 54, 11-14 (2005 |
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Title | Effect of Copper on the Uptake of Carbon from Medium to Daphnia magna |
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