Identification of the binding proteins of organic acid metabolites by matrix thermal shift assay

• Published in: Se pu = Chinese journal of chromatography Vol. 42; no. 7; pp. 702 - 710
• Main Authors: LI, Kejia, YE, Yuying, ZHANG, Xiaolei, ZHOU, Jiahua, LI, Yanan, YE, Mingliang
• Format: Journal Article
• Language: Chinese; English
• Published: China 01.07.2024
• Subjects: Citric Acid Cycle; Fumarates - chemistry; Fumarates - metabolism; HeLa Cells; Humans; Succinic Acid - chemistry; Succinic Acid - metabolism; succinate; binding proteins; matrix thermal shift assay (mTSA); lactate; organic acid metabolites; fumarate

Organic acid metabolites exhibit acidic properties. These metabolites serve as intermediates in major carbon metabolic pathways and are involved in several biochemical pathways, including the tricarboxylic acid (TCA) cycle and glycolysis. They also regulate cellular activity and play crucial roles i...