Functional characterization and application of a tightly regulated MekR/P mekA expression system in Escherichia coli and Pseudomonas putida

• Published in: Applied microbiology and biotechnology Vol. 97; no. 18; pp. 8239 - 8251
• Main Authors: Graf, Nadja, Altenbuchner, Josef
• Format: Journal Article
• Language: English
• Published: Germany 01.09.2013
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Base Sequence; Butanones - metabolism; Escherichia coli - genetics; Escherichia coli - metabolism; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Operon; Promoter Regions, Genetic; Pseudomonas - genetics; Pseudomonas putida - genetics; Pseudomonas putida - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription Initiation Site

A methyl ethyl ketone (MEK)-inducible system based on the broad-host-range plasmid pBBR1MCS2 and on the P mekA promoter region of the MEK degradation operon of Pseudomonas veronii MEK700 was characterized in Escherichia coli JM109 and Pseudomonas putida KT2440. For validation, β-galactosidase (lacZ)...