The ducky mutation in Cacna2d2 results in altered Purkinje cell morphology and is associated with the expression of a truncated alpha 2 delta-2 protein with abnormal function
The mouse mutant ducky, a model for absence epilepsy, is characterized by spike-wave seizures and cerebellar ataxia. A mutation in Cacna2d2, the gene encoding the alpha 2 delta-2 voltage-dependent calcium channel accessory subunit, has been found to underlie the ducky phenotype. The alpha 2 delta-2...
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Published in | The Journal of biological chemistry Vol. 277; no. 10; pp. 7684 - 7693 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
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United States
08.03.2002
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Abstract | The mouse mutant ducky, a model for absence epilepsy, is characterized by spike-wave seizures and cerebellar ataxia. A mutation in Cacna2d2, the gene encoding the alpha 2 delta-2 voltage-dependent calcium channel accessory subunit, has been found to underlie the ducky phenotype. The alpha 2 delta-2 mRNA is strongly expressed in cerebellar Purkinje cells. We show that du/du mice have abnormalities in their Purkinje cell dendritic tree. The mutation in alpha 2 delta-2 results in the introduction of a premature stop codon and predicts the expression of a truncated protein encoded by the first three exons of Cacna2d2, followed by 8 novel amino acids. We show that both mRNA and protein corresponding to this predicted transcript are expressed in du/du cerebellum and present in Purkinje cells. Whereas the alpha 2 delta-2 subunit increased the peak current density of the Ca(V)2.1/beta(4) channel combination when co-expressed in vitro, co-expression with the truncated mutant alpha 2 delta-2 protein reduced current density, indicating that it may contribute to the du phenotype. |
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AbstractList | The mouse mutant ducky, a model for absence epilepsy, is characterized by spike-wave seizures and cerebellar ataxia. A mutation in Cacna2d2, the gene encoding the alpha 2 delta-2 voltage-dependent calcium channel accessory subunit, has been found to underlie the ducky phenotype. The alpha 2 delta-2 mRNA is strongly expressed in cerebellar Purkinje cells. We show that du/du mice have abnormalities in their Purkinje cell dendritic tree. The mutation in alpha 2 delta-2 results in the introduction of a premature stop codon and predicts the expression of a truncated protein encoded by the first three exons of Cacna2d2, followed by 8 novel amino acids. We show that both mRNA and protein corresponding to this predicted transcript are expressed in du/du cerebellum and present in Purkinje cells. Whereas the alpha 2 delta-2 subunit increased the peak current density of the Ca(V)2.1/beta(4) channel combination when co-expressed in vitro, co-expression with the truncated mutant alpha 2 delta-2 protein reduced current density, indicating that it may contribute to the du phenotype. The mouse mutant ducky, a model for absence epilepsy, is characterized by spike-wave seizures and cerebellar ataxia. A mutation in Cacna2d2, the gene encoding the alpha 2 delta -2 voltage-dependent calcium channel accessory subunit, has been found to underlie the ducky phenotype. The alpha 2 delta -2 mRNA is strongly expressed in cerebellar Purkinje cells. We show that du/du mice have abnormalities in their Purkinje cell dendritic tree. The mutation in alpha 2 delta -2 results in the introduction of a premature stop codon and predicts the expression of a truncated protein encoded by the first three exons of Cacna2d2, followed by 8 novel amino acids. We show that both mRNA and protein corresponding to this predicted transcript are expressed in du/du cerebellum and present in Purkinje cells. Whereas the alpha 2 delta -2 subunit increased the peak current density of the Ca sub(V)2.1/ beta sub(4) channel combination when co-expressed in vitro, co-expression with the truncated mutant alpha 2 delta -2 protein reduced current density, indicating that it may contribute to the du phenotype. |
Author | Barclay, Jane Pratt, Wendy S Dolphin, Annette C Canti, Carles Brodbeck, Jens Meir, Alon Hunt, Steven P Moss, Fraser J Davies, Anthony Balaguero, Nuria Rees, Michele Page, Karen M Courtney, Jo-Maree |
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Snippet | The mouse mutant ducky, a model for absence epilepsy, is characterized by spike-wave seizures and cerebellar ataxia. A mutation in Cacna2d2, the gene encoding... |
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SubjectTerms | Alleles Amino Acid Sequence Amino Acids - chemistry Animals Biotin - analogs & derivatives Biotin - pharmacology Cacna2d2 gene Calcium Channels - chemistry Calcium Channels - genetics Calcium Channels, L-Type Cerebellum - metabolism Codon, Terminator COS Cells Dendrites - metabolism Disease Models, Animal DNA, Complementary - metabolism Electrophysiology Fluorescent Dyes - pharmacology Genotype Immunohistochemistry In Situ Hybridization Isoquinolines - pharmacology Kinetics Mice Models, Biological Molecular Sequence Data Mutation Phenotype Protein Structure, Tertiary Purkinje Cells - metabolism Rats RNA, Messenger - metabolism Time Factors Transfection Xenopus |
Title | The ducky mutation in Cacna2d2 results in altered Purkinje cell morphology and is associated with the expression of a truncated alpha 2 delta-2 protein with abnormal function |
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