Extracellular cellulolytic complexes production by microscopic fungi

• Published in: Biotechnologia acta Vol. 8; no. 5; pp. 78 - 85
• Main Authors: Syrchin, S O, Kharkevych, O S, Pavlychenko, A K, Yurieva, O M, Nakonechna, L T, Nekleva, Yu S, Kurchenko, I M
• Format: Journal Article
• Language: English
• Published: Kyiv Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine 01.01.2015; National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
• Subjects: endoglucanase; exoglucanase; Fusarium; Hypocrea jecorina; microscopic fungi; xylanase
• ISSN: 2410-7751; 1995-5537; 2410-776X
• DOI: 10.15407/biotech8.05.078

The aim of this work was to screen and to study the effect of inducers on the synthesis of the cellulolytic enzyme complexes by microscopic fungi. Cellulolytic and xylanolytic activities were determined by reducing sugar with DNS reagent, and B-glucosidase activity by pNPG hydrolysis. The enzyme preparations were obtained by ammonium sulphate precipitation. Among 32 studied strains of microscopic fungi 14 produced cellulo- and xylanolytic enzyme complexes. Fusarium sp. 5 and Fennellia sp. 2806 demonstrated the highest levels of all studied enzyme activities. Enzyme preparations with high endo-, exoglucanase, xylanase and B-glucosidase activities were obtained from these strains. Fusarium sp. 5 and Fennellia sp. 2806 were active producers of cellulase enzyme complexes during growth on natural substrates. It was shown that inductors of cellulolytic enzymes in Fusarium sp. 5 and Fennellia sp. 2806 differed from the ones in Trichoderma reesei.