The Active Site Topology of Aspergillus nigerEndopolygalacturonase II as Studied by Site-directed Mutagenesis

• Published in: The Journal of biological chemistry Vol. 275; no. 1; pp. 691 - 696
• Main Authors: Armand, Sylvie, Wagemaker, Matthé J.M., Sánchez-Torres, Paloma, Kester, Harry C.M., van Santen, Yovka, Dijkstra, Bauke W., Visser, Jaap, Benen, Jacques A.E.
• Format: Journal Article
• Language: English
• Published: American Society for Biochemistry and Molecular Biology 01.01.2000

Strictly conserved charged residues among polygalacturonases (Asp-180, Asp-201, Asp-202, His-223, Arg-256, and Lys-258) were subjected to site-directed mutagenesis in Aspergillus niger endopolygalacturonase II. Specific activity, product progression, and kinetic parameters ( K m and V max ) were det...