Complex formation by human insulin-like growth factor-binding protein-3 and human acid-labile subunit in growth hormone-deficient rats

• Published in: Endocrinology (Philadelphia) Vol. 134; no. 6; p. 2404
• Main Authors: Lewitt, M S, Saunders, H, Phuyal, J L, Baxter, R C
• Format: Journal Article
• Language: English
• Published: United States 01.06.1994
• Subjects: Animals; Carrier Proteins - administration & dosage; Carrier Proteins - metabolism; Chromatography; Glycoproteins - metabolism; Growth Hormone - deficiency; Half-Life; Humans; Hydrogen-Ion Concentration; Insulin-Like Growth Factor Binding Proteins; Insulin-Like Growth Factor I - administration & dosage; Insulin-Like Growth Factor I - metabolism; Kinetics; Molecular Weight; Rats

Insulin-like growth factor-binding protein-3 (IGFBP-3), after first associating with IGF-I or IGF-II, is able to associate with the acid-labile subunit (ALS) and form a 140-kDa complex. To investigate the factors regulating ternary complex formation in vivo, human (h) IGFBP-3, hIGF-I, and hALS were...