K562 Chronic Myeloid Leukemia Cells as a Dual β3-Expressing Functional Cell Line Model to Investigate the Effects of Combined αIIbβ3 and αvβ3 Antagonism

• Published in: Methods and protocols Vol. 8; no. 4; p. 73
• Main Authors: Elsharif, Amal A., Patterson, Laurence H., Shnyder, Steven D., Sheldrake, Helen M.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.07.2025; MDPI
• Subjects: Acetic acid; Angiogenesis; Antagonists; Antibodies; Antineoplastic drugs; Cancer; Cell adhesion; Cell adhesion & migration; Cell growth; Chronic myeloid leukemia; Extracellular matrix; Flow cytometry; Integrins; Leukemia; Ligands; Medical prognosis; Melanoma; Metastasis; Myeloid leukemia; Phorbol 12-myristate 13-acetate; Proteins; Tumors; United Kingdom > UK; United States > US; Germany; αIIbβ3; integrin αvβ3; cell-based functional assay; K562; PMA

Several of the integrin family of cell adhesion receptors have been popular targets for the development of anticancer agents, but with little clinical success to date. Cancer cells usually express multiple redundant integrins; one hypothesis for the lack of efficacy of current antagonists is their h...