A new mode for the diagnosis of angioimmunoblastic T-cell lymphoma

• Published in: Cellular and Molecular Biology Vol. 70; no. 5; pp. 155 - 160
• Main Authors: Wang, Hongxia, Han, Xiuhua, Nie, Yanhong, Zhang, Chunfang, Li, Jing, Yang, Rong, Jiang, Yajun
• Format: Journal Article
• Language: English
• Published: France 27.05.2024
• Subjects: Adult; Aged; Aged, 80 and over; Antigens, CD20 - analysis; Antigens, CD20 - metabolism; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; CD4 Antigens - metabolism; Cell Proliferation; Dendritic Cells, Follicular - metabolism; Dendritic Cells, Follicular - pathology; Enhancer of Zeste Homolog 2 Protein - metabolism; Female; Humans; Immunoblastic Lymphadenopathy - diagnosis; Immunoblastic Lymphadenopathy - pathology; Immunohistochemistry - methods; Lymphoma, T-Cell - diagnosis; Lymphoma, T-Cell - metabolism; Lymphoma, T-Cell - pathology; Lymphoma, T-Cell, Peripheral - diagnosis; Lymphoma, T-Cell, Peripheral - pathology; Male; Middle Aged; Neprilysin - metabolism; Programmed Cell Death 1 Receptor - metabolism; Proto-Oncogene Proteins c-bcl-6 - metabolism; Receptors, Complement 3d - analysis; Receptors, Complement 3d - metabolism; ROC Curve; Sensitivity and Specificity; T Follicular Helper Cells - immunology; T Follicular Helper Cells - metabolism
• DOI: 10.14715/cmb/2024.70.5.22
• ISSN: 0145-5680; 1165-158X

In order to explore a new mode for the diagnosis of angioimmunoblastic T-cell lymphoma (AITL), 31 cases of AITL and 28 cases of peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS) were used as the study subjects. Identifying T follicular helper (TFH) cells with CD4, CD10, Bcl-6, and PD-1, identifying proliferative B cells with CD20 and EZH2, identifying proliferative follicular dendritic cells (FDCs) with CD21 and CD23, and analyzing the value of TFH/B/FDC proliferation and immunolocalization in the diagnosis of AITL. (1) Outside the inherent lymphoid follicles, simultaneous proliferation of TFH/B/FDC (a new diagnostic mode) were observed in AITL [83.87%; 26/31], with their immunolocalizations in the same site [83.87%; 26/31], while this phenomenon was not observed in 28 cases of PTCL-NOS (P<0.05). (2) The sensitivity and specificity of using this new mode to diagnose AITL were both high (83.87%, 100%), which was superior to CD2 (100%, 0%), CD3 (100%, 0%), CD4 (100%, 32.14%), CD5 (100%, 25%), CD10 (61.9%, 100%), Bcl-6 (42.86%, 100%), PD-1 (83.87%, 96.43%), and its Youden Index (0.84) was the highest. The areas under the curve (AUC) of CD10, Bcl-6, PD-1, and new mode to diagnosis AITL were 0.81, 0.71, 0.90, and 0.92, respectively, while the new mode had the highest AUC. The simultaneous proliferation of TFH/B/FDC cells outside the inherent lymphoid follicles can be used to assist in the diagnosis of AITL, and the simultaneous spatiotemporal proliferation of TFH/B/FDC cells is a specific immunomorphology of AITL.