Lipid Extraction from Yeast Cells

The diversity of lipid molecules in biological tissues makes their analysis an experimental challenge. Not only do lipids differ greatly in their chemical structures and biophysical properties, but they also occur in greatly varying concentrations in living cells. Accordingly, even for an organism w...

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Bibliographic Details
Published inCold Spring Harbor protocols Vol. 2017; no. 5; p. pdb.prot085449
Main Authors Knittelfelder, Oskar L, Kohlwein, Sepp D
Format Journal Article
LanguageEnglish
Published United States 01.05.2017
Subjects
Centrifugation - methods
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Gas Chromatography-Mass Spectrometry
Glycerophospholipids - isolation & purification
Indicators and Reagents
Lipids - isolation & purification
Saccharomyces cerevisiae - chemistry
Yeasts - chemistry
Yeasts - growth & development
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Summary:The diversity of lipid molecules in biological tissues makes their analysis an experimental challenge. Not only do lipids differ greatly in their chemical structures and biophysical properties, but they also occur in greatly varying concentrations in living cells. Accordingly, even for an organism with a relatively simple lipidome such as yeast, multiple extraction and analysis protocols have been developed because none of them allows comprehensive and quantitative determination of all the diverse molecular lipid species. Here we describe an extraction procedure that results in good yields of neutral lipids and glycerophospholipids from yeast. The resulting samples are suitable for analysis by thin-layer chromatography, gas chromatography/mass spectrometry, or high-performance liquid chromatography.
ISSN:1559-6095
DOI:10.1101/pdb.prot085449